Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,118,723	C→G	8.1%	P800R (CCG→CGG)	PP_1884 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,118,723	0	C	G	8.1%	233.6 / 9.5	74	P800R (CCG→CGG)	PP_1884	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (36/32);  new base G (4/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

GGCAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTCG  >  NC_002947/2118579‑2118870                                                                                                                                                 |                                                                                                                                                    ggCAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGccgctgccgctg                                                                                                                                                 <  8:129884/149‑1 (MQ=255)  gCAGATCGATATCGTTGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCATACCAGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgg                                                                                                                                                >  1:304127/1‑149 (MQ=255)   cAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTggg                                                                                                                                               <  3:40890/149‑1 (MQ=255)        cgatatccatGAAGACGGCAAGCCTTACTACTAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTAACCCATCACGCTCGAGGGTTTCATCGTGCTGCAGCTGCCGCTGGGgcag                                                                                                                                           >  6:268642/1‑148 (MQ=255)         gatatggatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGAATTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGgcagca                                                                                                                                         <  6:280754/149‑1 (MQ=255)           tatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGgcagca                                                                                                                                         >  6:243912/1‑147 (MQ=255)           tatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGcaca                                                                                                                                       <  3:58466/149‑1 (MQ=255)            atcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAg                                                                                                                                      >  4:347597/1‑149 (MQ=255)               gatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTc                                                                                                                                    <  4:67480/148‑1 (MQ=255)                   aaGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGc                                                                                                                                <  4:51172/148‑1 (MQ=255)                    aGACGGCAATCCTTACTAGCAGGCCGACGCCGCGAGGCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgg                                                                                                                                                <  4:224481/131‑1 (MQ=255)                    aGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgg                                                                                                                                                >  3:224481/1‑131 (MQ=255)                    aGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCt                                                                                                                              <  7:31107/149‑1 (MQ=255)                             gCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAG‑CTTTGAGGCCCt                                                                                                                    >  3:277227/1‑149 (MQ=255)                                ttACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGcccgtt                                                                                                                  >  4:245700/1‑146 (MQ=255)                                  actacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGa                                                                                                                 <  3:96373/148‑1 (MQ=255)                                   ctacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAg                                                                                                                <  3:123583/148‑1 (MQ=255)                                    tacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCaa                                                                                                                                  >  4:107414/1‑129 (MQ=255)                                    tacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCaa                                                                                                                                  <  3:107414/129‑1 (MQ=255)                                         ggCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAgcaggcag                                                                                                         >  4:229615/1‑149 (MQ=255)                                             gACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAgcgc                                                                                                      >  3:355844/1‑148 (MQ=255)                                                gccgcGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACg                                                                                                  <  7:167250/149‑1 (MQ=255)                                                   gcgAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACggg                                                                                                >  5:375736/1‑148 (MQ=255)                                                          ccGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGcac                                                                                                                                        <  7:250736/101‑1 (MQ=255)                                                          ccGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGcac                                                                                                                                        >  8:250736/1‑101 (MQ=255)                                                          ccGCACATTTCTCCCTGGTTCGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCAACACGCTCGAGGCTTTCATCGTGCTGCCGCTGCCGCAGGGGCAGCACAGTCAACCCTATCAGGCCCTAGACCACCCAGCGCAACCCGCAAcgcg                                                                                         >  2:107192/1‑148 (MQ=255)                                                           cGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAAcgcg                                                                                         >  1:69048/1‑147 (MQ=255)                                                              acaTTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAgg                                                                                     >  5:168292/1‑148 (MQ=255)                                                                 tttCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGAAGGCAGAGCAACGGGCAACGCGCAGGTAGt                                                                                 >  8:249843/1‑149 (MQ=255)                                                                       cGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCg                                                                           >  6:159006/1‑149 (MQ=255)                                                                            ttggtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGa                                                                                                                 <  6:178462/106‑1 (MQ=255)                                                                            ttggtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGa                                                                                                                 >  5:178462/1‑106 (MQ=255)                                                                               gtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAg                                                                                      <  6:207242/130‑1 (MQ=255)                                                                               gtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAg                                                                                      >  5:207242/1‑130 (MQ=255)                                                                               gtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCa                                                                    <  4:355844/148‑1 (MQ=255)                                                                               gtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCAc                                                                   >  5:28165/1‑149 (MQ=255)                                                                                  cGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCt                                                                 >  1:284302/1‑148 (MQ=255)                                                                                    gTCTTGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTaaa                                                              <  3:229615/149‑1 (MQ=255)                                                                                    gTCTGTCGGCTCTCCCCCTACTAACCAATCCCGCGCTAGGGTTTAATCGTCCTGCCCCTGCCGCTGGGGAAGAAAAGTCATG‑CTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAc                                                             <  4:277227/149‑1 (MQ=255)                                                                                       tGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAcc                                                            >  1:346125/1‑148 (MQ=255)                                                                                         gAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAcc                                                            <  3:347597/146‑1 (MQ=255)                                                                                          aGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATa                                                        <  5:243912/149‑1 (MQ=255)                                                                                              tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAAcgc                                                                                          <  4:53947/111‑1 (MQ=255)                                                                                              tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAAcgc                                                                                          >  3:53947/1‑111 (MQ=255)                                                                                              tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCtt                                                    >  5:5931/1‑149 (MQ=255)                                                                                                  ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAc                                                             <  2:237590/136‑1 (MQ=255)                                                                                                  ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAc                                                             >  1:237590/1‑136 (MQ=255)                                                                                                  ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTc                                                  <  2:69048/147‑1 (MQ=255)                                                                                                  ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAg                                                >  3:243100/1‑149 (MQ=255)                                                                                                   cccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAg                                                >  3:129193/1‑148 (MQ=255)                                                                                                     cGACTTACCCATGTCGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCAc                                             <  5:268642/149‑1 (MQ=255)                                                                                                     cGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCAc                                             >  5:226104/1‑149 (MQ=255)                                                                                                            ccccTCACGCTCGAGGGGTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGtt                                      <  4:20435/149‑1 (MQ=255)                                                                                                            cccATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTGAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAAGGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGt                                       <  1:107192/148‑1 (MQ=255)                                                                                                            cccATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGt                                       <  6:5931/148‑1 (MQ=255)                                                                                                               aTCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTGAGCACTCCAGTTGAg                                   >  8:332664/1‑149 (MQ=255)                                                                                                               aTCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCCATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCCTACCTTGAAGCACTCCAGTTGAg                                   >  8:81586/1‑149 (MQ=255)                                                                                                                   cGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATa                                                        >  5:46872/1‑124 (MQ=255)                                                                                                                   cGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATa                                                        <  6:46872/124‑1 (MQ=255)                                                                                                                   cGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCggg                               <  7:249843/149‑1 (MQ=255)                                                                                                                     cTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGc                             <  6:28165/149‑1 (MQ=255)                                                                                                                      tCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCa                                                 <  4:350847/128‑1 (MQ=255)                                                                                                                      tCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCa                                                 >  3:350847/1‑128 (MQ=255)                                                                                                                          gggTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCAcat                        >  5:242413/1‑149 (MQ=255)                                                                                                                             tttCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCGTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCAcatcat                     >  8:9546/1‑149 (MQ=255)                                                                                                                             tttCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGGAGTGAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCAcatcat                     <  6:375736/149‑1 (MQ=255)                                                                                                                              ttCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATg                    >  2:276705/1‑149 (MQ=255)                                                                                                                                cATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGc                   <  5:159006/148‑1 (MQ=255)                                                                                                                                 aTCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGcc                  >  7:139913/1‑148 (MQ=255)                                                                                                                                   tgTGCTGCCCCAGTGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTAc               >  2:345190/2‑149 (MQ=255)                                                                                                                                   tgTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCa                                                                    >  8:82339/2‑96 (MQ=38)                                                                                                                                   tgTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCa                                                                    <  7:82339/95‑1 (MQ=38)                                                                                                                                    gTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTa                >  6:52441/1‑147 (MQ=255)                                                                                                                                    gTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTa                <  5:52441/147‑1 (MQ=255)                                                                                                                                     tgctgcCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTAcc              >  5:372517/1‑148 (MQ=255)                                                                                                                                       cttccgctgccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCaa            <  4:243100/145‑1 (MQ=255)                                                                                                                                       ctgccgctgccgCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCATGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCaa            <  6:226104/148‑1 (MQ=255)                                                                                                                                       ctgccgctgccgCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAAt           <  2:346125/149‑1 (MQ=255)                                                                                                                                        tgccgctgccgcTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAAt           >  3:53331/1‑148 (MQ=255)                                                                                                                                         tgtgctgccccagCGGCAGCACAGTCAAGCCTATCAGGCGCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCAc                                             >  5:78172/4‑113 (MQ=255)                                                                                                                                         tgtgctgccccagCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCAc                                             <  6:78172/110‑1 (MQ=255)                                                                                                                                         gccgctgccgctGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAAtt          >  4:31236/1‑148 (MQ=255)                                                                                                                                                ccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACctct    <  8:139913/147‑1 (MQ=255)                                                                                                                                                ccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTCg  <  4:53331/149‑1 (MQ=255)                                                                                                                                                ccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTCg  <  6:242413/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    GGCAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTCG  >  NC_002947/2118579‑2118870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑