Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,118,728 G→C 9.7% G802R (GGG→CGG)  PP_1884 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,118,7280GC 9.7% 232.1 / 11.3 80G802R (GGG→CGG) PP_1884hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/36);  new base C (5/3);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTCG  >  NC_002947/2118580‑2118870
                                                                                                                                                    |                                                                                                                                              
gCAGATCGATATCGTTGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCATACCAGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgg                                                                                                                                                >  1:304127/1‑149 (MQ=255)
 cAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTggg                                                                                                                                               <  3:40890/149‑1 (MQ=255)
      cgatatccatGAAGACGGCAAGCCTTACTACTAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTAACCCATCACGCTCGAGGGTTTCATCGTGCTGCAGCTGCCGCTGGGgcag                                                                                                                                           >  6:268642/1‑148 (MQ=255)
       gatatggatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGAATTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGgcagca                                                                                                                                         <  6:280754/149‑1 (MQ=255)
         tatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGgcagca                                                                                                                                         >  6:243912/1‑147 (MQ=255)
         tatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGcaca                                                                                                                                       <  3:58466/149‑1 (MQ=255)
          atcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAg                                                                                                                                      >  4:347597/1‑149 (MQ=255)
             gatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTc                                                                                                                                    <  4:67480/148‑1 (MQ=255)
                 aaGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGc                                                                                                                                <  4:51172/148‑1 (MQ=255)
                  aGACGGCAATCCTTACTAGCAGGCCGACGCCGCGAGGCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgg                                                                                                                                                <  4:224481/131‑1 (MQ=255)
                  aGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgg                                                                                                                                                >  3:224481/1‑131 (MQ=255)
                  aGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCt                                                                                                                              <  7:31107/149‑1 (MQ=255)
                           gCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAG‑CTTTGAGGCCCt                                                                                                                    >  3:277227/1‑149 (MQ=255)
                              ttACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGcccgtt                                                                                                                  >  4:245700/1‑146 (MQ=255)
                                actacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGa                                                                                                                 <  3:96373/148‑1 (MQ=255)
                                 ctacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAg                                                                                                                <  3:123583/148‑1 (MQ=255)
                                  tacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCaa                                                                                                                                  <  3:107414/129‑1 (MQ=255)
                                  tacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCaa                                                                                                                                  >  4:107414/1‑129 (MQ=255)
                                       ggCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAgcaggcag                                                                                                         >  4:229615/1‑149 (MQ=255)
                                           gACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAgcgc                                                                                                      >  3:355844/1‑148 (MQ=255)
                                              gccgcGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACg                                                                                                  <  7:167250/149‑1 (MQ=255)
                                                 gcgAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACggg                                                                                                >  5:375736/1‑148 (MQ=255)
                                                        ccGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGcac                                                                                                                                        <  7:250736/101‑1 (MQ=255)
                                                        ccGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGcac                                                                                                                                        >  8:250736/1‑101 (MQ=255)
                                                        ccGCACATTTCTCCCTGGTTCGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCAACACGCTCGAGGCTTTCATCGTGCTGCCGCTGCCGCAGGGGCAGCACAGTCAACCCTATCAGGCCCTAGACCACCCAGCGCAACCCGCAAcgcg                                                                                         >  2:107192/1‑148 (MQ=255)
                                                         cGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAAcgcg                                                                                         >  1:69048/1‑147 (MQ=255)
                                                            acaTTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAgg                                                                                     >  5:168292/1‑148 (MQ=255)
                                                               tttCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGAAGGCAGAGCAACGGGCAACGCGCAGGTAGt                                                                                 >  8:249843/1‑149 (MQ=255)
                                                                     cGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCg                                                                           >  6:159006/1‑149 (MQ=255)
                                                                          ttggtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGa                                                                                                                 <  6:178462/106‑1 (MQ=255)
                                                                          ttggtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGa                                                                                                                 >  5:178462/1‑106 (MQ=255)
                                                                             gtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAg                                                                                      <  6:207242/130‑1 (MQ=255)
                                                                             gtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAg                                                                                      >  5:207242/1‑130 (MQ=255)
                                                                             gtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCa                                                                    <  4:355844/148‑1 (MQ=255)
                                                                             gtGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCAc                                                                   >  5:28165/1‑149 (MQ=255)
                                                                                cGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCt                                                                 >  1:284302/1‑148 (MQ=255)
                                                                                  gTCTTGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTaaa                                                              <  3:229615/149‑1 (MQ=255)
                                                                                  gTCTGTCGGCTCTCCCCCTACTAACCAATCCCGCGCTAGGGTTTAATCGTCCTGCCCCTGCCGCTGGGGAAGAAAAGTCATG‑CTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAc                                                             <  4:277227/149‑1 (MQ=255)
                                                                                     tGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAcc                                                            >  1:346125/1‑148 (MQ=255)
                                                                                       gAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAcc                                                            <  3:347597/146‑1 (MQ=255)
                                                                                        aGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATa                                                        <  5:243912/149‑1 (MQ=255)
                                                                                            tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAAcgc                                                                                          >  3:53947/1‑111 (MQ=255)
                                                                                            tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAAcgc                                                                                          <  4:53947/111‑1 (MQ=255)
                                                                                            tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCtt                                                    >  5:5931/1‑149 (MQ=255)
                                                                                                ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAc                                                             >  1:237590/1‑136 (MQ=255)
                                                                                                ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAAc                                                             <  2:237590/136‑1 (MQ=255)
                                                                                                ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTc                                                  <  2:69048/147‑1 (MQ=255)
                                                                                                ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAg                                                >  3:243100/1‑149 (MQ=255)
                                                                                                 cccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAg                                                >  3:129193/1‑148 (MQ=255)
                                                                                                   cGACTTACCCATGTCGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCAc                                             <  5:268642/149‑1 (MQ=255)
                                                                                                   cGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCAc                                             >  5:226104/1‑149 (MQ=255)
                                                                                                          ccccTCACGCTCGAGGGGTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGtt                                      <  4:20435/149‑1 (MQ=255)
                                                                                                          cccATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTGAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAAGGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGt                                       <  1:107192/148‑1 (MQ=255)
                                                                                                          cccATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGt                                       <  6:5931/148‑1 (MQ=255)
                                                                                                             aTCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTGAGCACTCCAGTTGAg                                   >  8:332664/1‑149 (MQ=255)
                                                                                                             aTCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCCATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCCTACCTTGAAGCACTCCAGTTGAg                                   >  8:81586/1‑149 (MQ=255)
                                                                                                                 cGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATa                                                        >  5:46872/1‑124 (MQ=255)
                                                                                                                 cGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATa                                                        <  6:46872/124‑1 (MQ=255)
                                                                                                                 cGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCggg                               <  7:249843/149‑1 (MQ=255)
                                                                                                                   cTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGc                             <  6:28165/149‑1 (MQ=255)
                                                                                                                    tCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCa                                                 >  3:350847/1‑128 (MQ=255)
                                                                                                                    tCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCa                                                 <  4:350847/128‑1 (MQ=255)
                                                                                                                        gggTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCAcat                        >  5:242413/1‑149 (MQ=255)
                                                                                                                           tttCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCGTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCAcatcat                     >  8:9546/1‑149 (MQ=255)
                                                                                                                           tttCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGGAGTGAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCAcatcat                     <  6:375736/149‑1 (MQ=255)
                                                                                                                            ttCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATg                    >  2:276705/1‑149 (MQ=255)
                                                                                                                              cATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGc                   <  5:159006/148‑1 (MQ=255)
                                                                                                                               aTCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGcc                  >  7:139913/1‑148 (MQ=255)
                                                                                                                                 tgTGCTGCCCCAGTGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTAc               >  2:345190/2‑149 (MQ=255)
                                                                                                                                 tgTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCa                                                                    >  8:82339/2‑96 (MQ=38)
                                                                                                                                 tgTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCa                                                                    <  7:82339/95‑1 (MQ=38)
                                                                                                                                  gTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTa                >  6:52441/1‑147 (MQ=255)
                                                                                                                                  gTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTa                <  5:52441/147‑1 (MQ=255)
                                                                                                                                   tgctgcCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTAcc              >  5:372517/1‑148 (MQ=255)
                                                                                                                                     cttccgctgccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCaa            <  4:243100/145‑1 (MQ=255)
                                                                                                                                     ctgccgctgccgCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCATGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCaa            <  6:226104/148‑1 (MQ=255)
                                                                                                                                     ctgccgctgccgCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAAt           <  2:346125/149‑1 (MQ=255)
                                                                                                                                      tgccgctgccgcTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAAt           >  3:53331/1‑148 (MQ=255)
                                                                                                                                       tgtgctgccccagCGGCAGCACAGTCAAGCCTATCAGGCGCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCAc                                             >  5:78172/4‑113 (MQ=255)
                                                                                                                                       tgtgctgccccagCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCAc                                             <  6:78172/110‑1 (MQ=255)
                                                                                                                                       gccgctgccgctGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAAtt          >  4:31236/1‑148 (MQ=255)
                                                                                                                                              ccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACctct    <  8:139913/147‑1 (MQ=255)
                                                                                                                                              ccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTCg  <  4:53331/149‑1 (MQ=255)
                                                                                                                                              ccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTCg  <  6:242413/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GCAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTCG  >  NC_002947/2118580‑2118870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑