Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,138,409 T→G 9.0% L171R (CTG→CGG)  yadG → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,138,4090TG 9.0% 150.7 / 6.3 55L171R (CTG→CGG) yadGABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (25/25);  new base G (2/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

TCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAGCATG  >  NC_002947/2138263‑2138557
                                                                                                                                                  |                                                                                                                                                    
tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTgc                                                                                                                                                    >  8:72262/1‑149 (MQ=255)
             tgGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGAtgt                                                                                                                                        <  8:329721/148‑1 (MQ=255)
             tgGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAta                                                                                                                                         <  8:272821/147‑2 (MQ=255)
              gggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAt                                                                                                                                          <  3:292534/145‑1 (MQ=255)
              gggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAt                                                                                                                                          >  4:292534/1‑145 (MQ=255)
               ggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTgg                                                                                                                                      <  3:361193/148‑1 (MQ=255)
                 aCAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCGGCTGATCCTGGACGAACCAACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGc                                                                                                                                   >  8:345725/1‑149 (MQ=255)
                     aCGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTcc                                                                                                                               >  5:22052/1‑149 (MQ=255)
                      cGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCt                                                                                                                              <  7:24175/149‑1 (MQ=255)
                       gTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTc                                                                                                                             <  2:179338/149‑1 (MQ=255)
                          aTGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACt                                                                                                                          <  7:152505/149‑1 (MQ=255)
                                aGTCGCGTTCGTTGTCCGGAGGCATGAAGCGGCGCCTGATGATTGCCCGCGTATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCt                                                                                                                     >  8:129242/1‑148 (MQ=255)
                                aGTCGCGTTCGATGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGTGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGGTg                                                                                                                    <  8:50993/149‑1 (MQ=255)
                                 gTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTg                                                                                                                    >  5:46393/1‑148 (MQ=255)
                                  tCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGa                                                                                                                   <  6:114704/148‑1 (MQ=255)
                                  tCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGa                                                                                                                   >  5:114704/1‑148 (MQ=255)
                                   cgcgTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAAc                                                                                                                 <  8:171830/149‑1 (MQ=255)
                                      gttcgtcGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAAc                                                                                                                 >  8:255656/1‑146 (MQ=255)
                                         cgttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGaa                                                                                                            <  8:162678/148‑1 (MQ=255)
                                          gttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAg                                                                                                           <  3:260844/148‑1 (MQ=255)
                                                       aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATc                                                                                                     <  4:295225/141‑1 (MQ=255)
                                                       aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATc                                                                                                     >  5:161700/1‑141 (MQ=255)
                                                       aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATc                                                                                                     >  3:295225/1‑141 (MQ=255)
                                                       aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATc                                                                                                     <  6:161700/141‑1 (MQ=255)
                                                               gcgcCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACcatc                                                                                               >  4:177071/1‑139 (MQ=255)
                                                               gcgcCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACcatc                                                                                               <  3:177071/139‑1 (MQ=255)
                                                                 gcCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCaccacc                                                                                   >  7:147982/1‑149 (MQ=255)
                                                                  ccTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCAccaccc                                                                                  <  7:255656/149‑1 (MQ=255)
                                                                    tgatgaTTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACTAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTc                                                                                                                                <  1:130156/101‑1 (MQ=255)
                                                                    tgatgaTTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGAAGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTc                                                                                                                                >  2:130156/1‑101 (MQ=255)
                                                                     gatgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTcacc                                                                                      >  6:320939/1‑142 (MQ=255)
                                                                     gatgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTcacc                                                                                      <  5:320939/142‑1 (MQ=255)
                                                                      atgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACt                                                                               <  6:46393/148‑1 (MQ=255)
                                                                             cccGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTgg                                                                         >  6:345996/1‑147 (MQ=255)
                                                                             cccGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACCAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCATCACTGAGCTGAACCAGAAg                                                                                                           >  5:323824/1‑113 (MQ=255)
                                                                               cgcgTATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAaga                                                                     <  7:129242/149‑1 (MQ=255)
                                                                                 cgcATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAgg                                                                   <  7:89741/149‑1 (MQ=255)
                                                                                         tCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGagcag                                                           >  4:74862/1‑149 (MQ=255)
                                                                                           cACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCt                                                         >  6:198712/1‑149 (MQ=255)
                                                                                             cGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtgt                                                       <  8:147982/149‑1 (MQ=255)
                                                                                                 ccGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCg                                                   >  3:168055/1‑149 (MQ=255)
                                                                                                        tgctgATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACGTc                                            >  6:321093/1‑149 (MQ=255)
                                                                                                                   gACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGa                                 <  7:72262/149‑1 (MQ=255)
                                                                                                                       aaCCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCAt                             <  4:168055/149‑1 (MQ=255)
                                                                                                                          ccACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATgg                           >  8:54803/1‑148 (MQ=255)
                                                                                                                            aCCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCAc                        >  2:48170/1‑149 (MQ=255)
                                                                                                                              cgccgGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCa                      >  7:159048/1‑149 (MQ=255)
                                                                                                                                   gggTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCAtcgtc                 <  6:22052/149‑1 (MQ=255)
                                                                                                                                      tGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCgag              <  5:345996/149‑1 (MQ=255)
                                                                                                                                        gATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCAACCTCGAGaa            >  5:225696/1‑149 (MQ=255)
                                                                                                                                         acatCGAGCGGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGc                                         <  7:306244/117‑1 (MQ=255)
                                                                                                                                         acatCGAGCGGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGc                                         >  8:306244/3‑119 (MQ=255)
                                                                                                                                          tatCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAaca          >  3:203706/1‑149 (MQ=255)
                                                                                                                                            tCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAacac         >  2:3811/1‑148 (MQ=255)
                                                                                                                                            tCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACAcc        <  5:198712/149‑1 (MQ=255)
                                                                                                                                                  tGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGAACATGGCACCATCGGCGAGAACACCAGCATg  >  7:297535/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAGCATG  >  NC_002947/2138263‑2138557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑