Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,138,414 C→A 9.1% R173S (CGC→AGC)  yadG → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,138,4140CA 9.1% 155.6 / 7.0 55R173S (CGC→AGC) yadGABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base A (2/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

TGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAGCATGC  >  NC_002947/2138276‑2138558
                                                                                                                                          |                                                                                                                                                
tgGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGAtgt                                                                                                                                         <  8:329721/148‑1 (MQ=255)
tgGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAta                                                                                                                                          <  8:272821/147‑2 (MQ=255)
 gggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAt                                                                                                                                           <  3:292534/145‑1 (MQ=255)
 gggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAt                                                                                                                                           >  4:292534/1‑145 (MQ=255)
  ggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTgg                                                                                                                                       <  3:361193/148‑1 (MQ=255)
    aCAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCGGCTGATCCTGGACGAACCAACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGc                                                                                                                                    >  8:345725/1‑149 (MQ=255)
        aCGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTcc                                                                                                                                >  5:22052/1‑149 (MQ=255)
         cGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCt                                                                                                                               <  7:24175/149‑1 (MQ=255)
          gTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTc                                                                                                                              <  2:179338/149‑1 (MQ=255)
             aTGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACt                                                                                                                           <  7:152505/149‑1 (MQ=255)
                   aGTCGCGTTCGTTGTCCGGAGGCATGAAGCGGCGCCTGATGATTGCCCGCGTATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCt                                                                                                                      >  8:129242/1‑148 (MQ=255)
                   aGTCGCGTTCGATGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGTGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGGTg                                                                                                                     <  8:50993/149‑1 (MQ=255)
                    gTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTg                                                                                                                     >  5:46393/1‑148 (MQ=255)
                     tCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGa                                                                                                                    <  6:114704/148‑1 (MQ=255)
                     tCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGa                                                                                                                    >  5:114704/1‑148 (MQ=255)
                      cgcgTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAAc                                                                                                                  <  8:171830/149‑1 (MQ=255)
                         gttcgtcGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAAc                                                                                                                  >  8:255656/1‑146 (MQ=255)
                            cgttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGaa                                                                                                             <  8:162678/148‑1 (MQ=255)
                             gttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAg                                                                                                            <  3:260844/148‑1 (MQ=255)
                                          aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATc                                                                                                      <  4:295225/141‑1 (MQ=255)
                                          aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATc                                                                                                      >  3:295225/1‑141 (MQ=255)
                                          aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATc                                                                                                      >  5:161700/1‑141 (MQ=255)
                                          aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATc                                                                                                      <  6:161700/141‑1 (MQ=255)
                                                  gcgcCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACcatc                                                                                                >  4:177071/1‑139 (MQ=255)
                                                  gcgcCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACcatc                                                                                                <  3:177071/139‑1 (MQ=255)
                                                    gcCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCaccacc                                                                                    >  7:147982/1‑149 (MQ=255)
                                                     ccTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCAccaccc                                                                                   <  7:255656/149‑1 (MQ=255)
                                                       tgatgaTTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACTAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTc                                                                                                                                 <  1:130156/101‑1 (MQ=255)
                                                       tgatgaTTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGAAGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTc                                                                                                                                 >  2:130156/1‑101 (MQ=255)
                                                        gatgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTcacc                                                                                       <  5:320939/142‑1 (MQ=255)
                                                        gatgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTcacc                                                                                       >  6:320939/1‑142 (MQ=255)
                                                         atgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACt                                                                                <  6:46393/148‑1 (MQ=255)
                                                                cccGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTgg                                                                          >  6:345996/1‑147 (MQ=255)
                                                                cccGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACCAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCATCACTGAGCTGAACCAGAAg                                                                                                            >  5:323824/1‑113 (MQ=255)
                                                                  cgcgTATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAaga                                                                      <  7:129242/149‑1 (MQ=255)
                                                                    cgcATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAgg                                                                    <  7:89741/149‑1 (MQ=255)
                                                                            tCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGagcag                                                            >  4:74862/1‑149 (MQ=255)
                                                                              cACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCt                                                          >  6:198712/1‑149 (MQ=255)
                                                                                cGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtgt                                                        <  8:147982/149‑1 (MQ=255)
                                                                                    ccGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCg                                                    >  3:168055/1‑149 (MQ=255)
                                                                                           tgctgATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACGTc                                             >  6:321093/1‑149 (MQ=255)
                                                                                                      gACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGa                                  <  7:72262/149‑1 (MQ=255)
                                                                                                          aaCCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCAt                              <  4:168055/149‑1 (MQ=255)
                                                                                                             ccACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATgg                            >  8:54803/1‑148 (MQ=255)
                                                                                                               aCCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCAc                         >  2:48170/1‑149 (MQ=255)
                                                                                                                 cgccgGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCa                       >  7:159048/1‑149 (MQ=255)
                                                                                                                      gggTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCAtcgtc                  <  6:22052/149‑1 (MQ=255)
                                                                                                                         tGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCgag               <  5:345996/149‑1 (MQ=255)
                                                                                                                           gATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCAACCTCGAGaa             >  5:225696/1‑149 (MQ=255)
                                                                                                                            acatCGAGCGGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGc                                          <  7:306244/117‑1 (MQ=255)
                                                                                                                            acatCGAGCGGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGc                                          >  8:306244/3‑119 (MQ=255)
                                                                                                                             tatCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAaca           >  3:203706/1‑149 (MQ=255)
                                                                                                                               tCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAacac          >  2:3811/1‑148 (MQ=255)
                                                                                                                               tCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACAcc         <  5:198712/149‑1 (MQ=255)
                                                                                                                                     tGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGAACATGGCACCATCGGCGAGAACACCAGCATg   >  7:297535/1‑149 (MQ=255)
                                                                                                                                       cgccgcTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAGCATgc  <  1:48170/148‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                
TGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAGCATGC  >  NC_002947/2138276‑2138558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑