Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,155,104 C→T 6.6% A91A (GCC→GCT)  PP_1910 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,155,1040CT 6.6% 229.0 / 4.1 76A91A (GCC→GCT) PP_1910hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/37);  new base T (3/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

GTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGGAAGAAT  >  NC_002947/2154959‑2155252
                                                                                                                                                 |                                                                                                                                                    
gTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCgtg                                                                                                                                                   <  3:73976/149‑1 (MQ=255)
gTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCgtg                                                                                                                                                   <  3:79184/149‑1 (MQ=255)
    ggTGCAGGCGAAGTTCGATTTTGAACGAGATGAGCAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCgtggtg                                                                                                                                                >  1:289693/1‑148 (MQ=255)
    ggTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGa                                                                                                                                               <  8:354995/149‑1 (MQ=255)
     gTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCgtggtg                                                                                                                                                >  4:181439/1‑147 (MQ=255)
         aGGCGAAGTTCGATTTTGAACGAGATGAGCAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAaggag                                                                                                                                          <  2:289693/149‑1 (MQ=255)
          ggCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAaggagg                                                                                                                                         <  2:189489/149‑1 (MQ=255)
          ggCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAagga                                                                                                                                           <  5:174678/147‑1 (MQ=255)
          ggCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGAAGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAagga                                                                                                                                           >  6:174678/1‑147 (MQ=255)
              aaGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGc                                                                                                                                     >  7:136617/1‑149 (MQ=255)
                   cGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAaggag                                                                                                                                          <  8:317266/139‑1 (MQ=255)
                        ttGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCa                                                                                                                            <  1:190678/148‑1 (MQ=255)
                           aaCGAGATGATCAGGAGGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCTAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAagga                                                                                                                                           <  4:17701/130‑1 (MQ=255)
                              tagaTGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTg                                                                                                                     <  5:72272/148‑1 (MQ=255)
                                gaTGAACAGCACGTGGTGGTTATACACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTg                                                                                                                     >  6:72272/1‑147 (MQ=255)
                                      cAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAgg                                                                                                             <  8:86549/149‑1 (MQ=255)
                                        gCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCt                                                                                                           <  4:356793/149‑1 (MQ=255)
                                        gCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCt                                                                                                           <  3:16206/149‑1 (MQ=255)
                                        gCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCt                                                                                                           <  4:362535/149‑1 (MQ=255)
                                          aCGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTAt                                                                                                         <  8:136617/149‑1 (MQ=255)
                                            gtggtgGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCg                                                                                                                        <  8:264059/132‑1 (MQ=255)
                                            gtggtgGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCg                                                                                                                        >  7:264059/1‑132 (MQ=255)
                                                  gtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCt                                                                                                 >  4:27332/1‑149 (MQ=255)
                                                  gtTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCt                                                                                                 <  3:27332/149‑1 (MQ=255)
                                                   ttATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGATGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTg                                                                                                <  1:81212/149‑1 (MQ=255)
                                                      tCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGaa                                                                                             >  4:119617/1‑149 (MQ=255)
                                                      tCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGa                                                                                              <  4:323332/148‑1 (MQ=255)
                                                       ccACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAAc                                                                                            >  5:60709/1‑149 (MQ=255)
                                                         acaGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTg                                                                                          >  6:39564/1‑149 (MQ=255)
                                                                   ggACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATc                                                                                >  2:193372/1‑149 (MQ=255)
                                                                       gTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCttt                                                                            >  4:360219/1‑149 (MQ=255)
                                                                        tCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTg                                                                           <  6:60709/149‑1 (MQ=255)
                                                                        tCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTg                                                                           <  1:4488/149‑1 (MQ=255)
                                                                           aaGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGa                                                                         >  7:263986/1‑148 (MQ=255)
                                                                                aaGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTg                                                                     >  8:149581/1‑147 (MQ=255)
                                                                                 aGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAg                                                                  <  3:360219/149‑1 (MQ=255)
                                                                                 aGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCAAAGGATCttcgc                                                                  <  3:196214/149‑5 (MQ=255)
                                                                                  gATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAgg                                                                 >  1:146333/1‑149 (MQ=255)
                                                                                  gATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAgg                                                                 >  1:184865/1‑149 (MQ=255)
                                                                                  gATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGACGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGAGAAGATCCTTTGGATCTGCAg                                                                  >  7:92244/1‑148 (MQ=255)
                                                                                      gTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAtt                                                             >  3:332631/1‑149 (MQ=255)
                                                                                      gTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCAt                                                              <  3:181439/148‑1 (MQ=255)
                                                                                            cAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTg                                                                           <  7:196170/129‑1 (MQ=255)
                                                                                            cAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTg                                                                           >  8:196170/1‑129 (MQ=255)
                                                                                               cGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCgag                                                      >  3:320563/1‑147 (MQ=255)
                                                                                                    tCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGc                                               >  6:231619/1‑149 (MQ=255)
                                                                                                      ttGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGaaa                                                                                                               <  8:31283/83‑1 (MQ=255)
                                                                                                      ttGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtt                                             >  7:296703/1‑149 (MQ=255)
                                                                                                      ttGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtt                                             >  6:91301/1‑149 (MQ=255)
                                                                                                      ttGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtt                                             >  1:66403/1‑149 (MQ=255)
                                                                                                            tGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTc                                       >  1:167950/1‑149 (MQ=255)
                                                                                                              gTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGc                                     >  3:143082/1‑149 (MQ=255)
                                                                                                                 aCCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtttt                                           <  3:150423/140‑1 (MQ=255)
                                                                                                                 aCCCTACCGATCCACAGCGAAAGTACATACGCCGTGGTGAAGGAGGGTGCGAATACACAGTCGTTGCCTAAAGGAAATGACGTGCTGTAACTGGGCGAAGATCCATTGGATCTGCAGGCATACTTCGAGGAGGAGCTTTTgagatctg                                   >  4:150423/1‑141 (MQ=255)
                                                                                                                  cccTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCttt                                                                            >  3:74994/1‑106 (MQ=255)
                                                                                                                  cccTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCttt                                                                            <  4:74994/106‑1 (MQ=255)
                                                                                                                   ccTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtt                                             <  5:283097/136‑1 (MQ=255)
                                                                                                                   ccTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCtt                                             >  6:283097/1‑136 (MQ=255)
                                                                                                                     tACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCga                                                       <  1:366363/124‑1 (MQ=255)
                                                                                                                     tACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCga                                                       >  2:366363/1‑124 (MQ=255)
                                                                                                                          aTCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGt                         <  5:231619/149‑1 (MQ=255)
                                                                                                                           accACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGc                                <  4:295101/140‑1 (MQ=255)
                                                                                                                           accACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCagatcgg                        >  3:295101/2‑143 (MQ=255)
                                                                                                                            ccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTg                                          <  5:241321/130‑1 (MQ=255)
                                                                                                                            ccACGGCGTATGTACATTCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTg                                          >  6:241321/1‑130 (MQ=255)
                                                                                                                              acaGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTgg                                                                          >  5:183833/1‑96 (MQ=255)
                                                                                                                              acaGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTgg                                                                          <  6:183833/96‑1 (MQ=255)
                                                                                                                              acaGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCt                     <  4:332631/149‑1 (MQ=255)
                                                                                                                                aGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGc                   <  2:184865/149‑1 (MQ=255)
                                                                                                                                  cGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCtc                 <  5:39564/149‑1 (MQ=255)
                                                                                                                                   gAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCtca                <  4:320563/149‑1 (MQ=255)
                                                                                                                                   gAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGACCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCtca                >  7:243089/1‑149 (MQ=255)
                                                                                                                                         aCATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATcc          >  2:188438/1‑149 (MQ=255)
                                                                                                                                             aCGCTGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCgg        >  5:367710/1‑147 (MQ=255)
                                                                                                                                              cGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCgg        >  1:74258/1‑146 (MQ=255)
                                                                                                                                              cGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGgaa      <  2:66403/148‑1 (MQ=255)
                                                                                                                                                 cGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGGAAGAAt  <  7:149581/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTACGGTGCAGGCGAAGTTCGATTTTGAACGAGATGAACAGCACGTGGTGGTTATCCACAGCGAGCTGGACGTCGAAGTCAAGATGGTTTGCCAGCGTTGTCTTGAGCTGGTCACCCTACCGATCCACAGCGAATGTACATACGCCGTGGTGAAGGAGGGTGCGAATACCCAGTCGTTGCCGAAAGGCTATGACGTGCTGGAACTGGGCGAAGATCCTTTGGATCTGCAGGCATTGGTCGAGGAGGAGCTTTTGCTCGCCTTGCCAATCGTGCCTGCTCATCATCCGGAAGAAT  >  NC_002947/2154959‑2155252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑