Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,189,575	G→A	7.1%	A148T (GCT→ACT) ‡	PP_1937 →	helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,189,575	0	G	A	7.1%	251.9 / 5.0	85	A148T (GCT→ACT) ‡	PP_1937	helicase
Reads supporting (aligned to +/- strand):  ref base G (36/42);  new base A (3/3);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAATGCGAGAGTACTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGG  >  NC_002947/2189428‑2189717                                                                                                                                                    |                                                                                                                                                gAAATGCGAGAGTACTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGc                                                                                                                                                <  3:264747/149‑1 (MQ=255)   aaTGCGAGAGTACTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTg                                                                                                                                              >  8:195765/1‑149 (MQ=255)    aTGCGAGAGTACTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTTCAGACGGCTACATGCAAAAATGTACTGCTCCGCTg                                                                                                                                              >  5:265450/1‑148 (MQ=255)         agagTACTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAg                                                                                                                                        <  6:95997/149‑1 (MQ=255)          gagTACTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGt                                                                                                                                       <  3:284005/149‑1 (MQ=255)            gTACTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCAGCGGAGCAGta                                                                                                                                      <  4:158185/148‑2 (MQ=255)               cTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGc                                                                                                                                  >  4:294239/1‑149 (MQ=255)               cTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGc                                                                                                                                  <  1:56249/149‑1 (MQ=255)                tGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCt                                                                                                                                 >  5:69774/1‑149 (MQ=255)                  gCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCtt                                                                                                                                <  4:149869/148‑1 (MQ=255)                    tCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTggg                                                                                                                             >  6:124456/1‑149 (MQ=255)                    tCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTggg                                                                                                                             >  2:72628/1‑149 (MQ=255)                     cAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAg                                                                                                                                           <  5:122017/134‑1 (MQ=255)                     cAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAg                                                                                                                                           >  6:122017/1‑134 (MQ=255)                      aaGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGt                                                                                                                            <  6:265450/148‑1 (MQ=255)                                  tcGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTacac                                                                                                               >  4:318876/1‑149 (MQ=255)                                  tcGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTacac                                                                                                               >  1:85341/1‑149 (MQ=255)                                    gCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTacac                                                                                                               <  3:59182/147‑1 (MQ=255)                                    gCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTg                                                                                                             <  6:356360/149‑1 (MQ=255)                                    gCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTg                                                                                                             <  7:195765/149‑1 (MQ=255)                                    gCTAGCGCTGTGTGGCCAAGTACTACATTGCAGCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTg                                                                                                             <  1:72628/149‑1 (MQ=255)                                      tAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGa                                                                                                           >  4:320715/1‑149 (MQ=255)                                           cTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAgg                                                                                                      <  5:124456/149‑1 (MQ=255)                                            tgtgtgGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACg                                                                                                                                   <  1:140403/119‑1 (MQ=255)                                            tgtgtgGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACg                                                                                                                                   >  2:140403/1‑119 (MQ=255)                                                tgGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑Ta                                                                                                 <  3:294239/148‑1 (MQ=255)                                                tgGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑Ta                                                                                                 <  3:318876/148‑1 (MQ=255)                                                  gCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGTTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACa                                                                                               >  4:263111/1‑148 (MQ=255)                                                          ctacATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCa                                                                                      <  3:320715/149‑1 (MQ=255)                                                          ctacATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCa                                                                                      <  4:330749/149‑1 (MQ=255)                                                               ttGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAg                                                                                 >  2:317431/1‑149 (MQ=255)                                                                    tCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAAc                                                                            >  7:335040/1‑149 (MQ=255)                                                                      gAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGt                                                                          >  6:253665/1‑149 (MQ=255)                                                                        gCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTgc                                                                        <  6:69774/149‑1 (MQ=255)                                                                         cTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGGACGTgcg                                                                       >  4:331602/1‑149 (MQ=255)                                                                             ttCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCtt                                                                    >  5:372872/1‑148 (MQ=255)                                                                             ttCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACAGCTCCCCTGGAGCAGTGACGCTTTGGTCCAGCAACTACACTGGACCAGGTT‑TACAAACTCAGCATGAATCGAACGTGCGCtt                                                                    >  1:278986/1‑148 (MQ=255)                                                                                gcgcCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTTATACATACTCAGCATGAAGCGCACGTTCGCTTCat                                                                 <  1:50054/149‑2 (MQ=255)                                                                                gcgcCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGCACTTTCGCTTCatg                                                                <  8:17957/149‑3 (MQ=255)                                                                                gcgcCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGCACGTTCGCTTCat                                                                 >  7:17957/1‑147 (MQ=255)                                                                                gcgcCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCAcc                                                                >  3:325988/1‑149 (MQ=255)                                                                                     ggTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTgg                                                           <  6:131826/149‑1 (MQ=255)                                                                                     ggTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTgg                                                           <  8:158559/149‑1 (MQ=255)                                                                                         aCGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGa                                                        >  5:274760/1‑148 (MQ=255)                                                                                           gTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACTTGCGCTTCACCTCTGGGGa                                                        >  2:290855/1‑146 (MQ=255)                                                                                            tGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAaga                                                    <  8:335040/149‑1 (MQ=255)                                                                                              cGAATTCGTTACCCGACAGGGTACAGACGGTTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAgc                                                  <  3:263111/149‑1 (MQ=255)                                                                                              cGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAagag                                                   <  7:127709/148‑1 (MQ=255)                                                                                               gAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAgcg                                                 >  4:21750/1‑149 (MQ=255)                                                                                                aaTTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCAc                                                                 <  8:291200/132‑1 (MQ=255)                                                                                                aaTTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCAc                                                                 >  7:291200/1‑132 (MQ=255)                                                                                                     gTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACTCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCttt                                           <  8:300105/149‑1 (MQ=255)                                                                                                     gTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAaga                                                    >  4:325808/1‑140 (MQ=255)                                                                                                     gTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAaga                                                    <  3:325808/140‑1 (MQ=255)                                                                                                     gTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCtt                                            <  1:317431/148‑1 (MQ=255)                                                                                                          ccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTttgtt                                        <  5:219336/147‑1 (MQ=255)                                                                                                          ccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTttgtt                                        >  6:219336/1‑147 (MQ=255)                                                                                                          ccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTttgtt                                        <  1:247629/147‑1 (MQ=255)                                                                                                          ccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTttgtt                                        >  5:137708/1‑147 (MQ=255)                                                                                                          ccGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTttgtt                                        >  2:247629/1‑147 (MQ=255)                                                                                                              cAGGGTACAGACTGCTACATGCAAAAATGTACTGCTCCGCTTGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGAcc                                  <  2:278986/149‑1 (MQ=255)                                                                                                              cAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGAcc                                  <  6:372872/149‑1 (MQ=255)                                                                                                                 ggTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAAt                               <  6:274760/149‑1 (MQ=255)                                                                                                                  gTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCT‑GGGAAAGAGCGCGCTTTGTTGAGACCAAtct                             >  4:127307/1‑149 (MQ=255)                                                                                                                        aCGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAgc                                                  <  4:144532/123‑1 (MQ=255)                                                                                                                                aTGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTa                         <  8:348573/140‑1 (MQ=255)                                                                                                                                aTGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTa                         >  7:348573/1‑140 (MQ=255)                                                                                                                                 tGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCt               >  1:236470/1‑149 (MQ=255)                                                                                                                                    aaaaaTGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCtttt            >  3:155542/1‑149 (MQ=255)                                                                                                                                     aataTGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTg           <  1:290855/146‑1 (MQ=255)                                                                                                                                     aaaaTGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTg           <  4:325988/149‑1 (MQ=255)                                                                                                                                     aaaaTGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTg           <  3:21750/149‑1 (MQ=255)                                                                                                                                       gcgtcaCTGCTCCAGCGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGGGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTg           >  5:207606/6‑147 (MQ=255)                                                                                                                                         gtcaCTGCTCCAGCGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACGAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACGTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGc       >  3:234025/4‑149 (MQ=255)                                                                                                                                         gtcaCTGCTCCAGCGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTa                         <  4:356869/128‑1 (MQ=255)                                                                                                                                         gtcaCTGCTCCAGCGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTa                         >  3:356869/4‑131 (MQ=255)                                                                                                                                          gTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCt      >  7:10417/1‑149 (MQ=255)                                                                                                                                            aCTGCTCCAGCGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTg    <  4:234025/149‑1 (MQ=255)                                                                                                                                                cTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGGACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTggg  <  3:331602/147‑1 (MQ=255)                                                                                                                                                cTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGa                    >  2:3517/1‑129 (MQ=255)                                                                                                                                                cTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGa                    <  1:3517/129‑1 (MQ=255)                                                                                                                                                cTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTggg  <  4:157958/147‑1 (MQ=255)                                                                                                                                                cTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTggg  >  2:79703/1‑147 (MQ=255)                                                                                                                                                 tCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTggg  >  1:221584/1‑146 (MQ=255)                                                                                                                                                 tCCGCTGGAGCAGTCACGCTTGTGTAAAGCAACTACACTGGACCACGTT‑TACATACTCAGCATGAAGAGAACGTGCGCTACACCTCTGGGGAAAGACCGCGCTTTGTTGAGACCAATCTCCTAGATCAGTGCTTTTGGATCTTggg  >  8:279761/1‑146 (MQ=255)                                                                                                                                                    |                                                                                                                                                GAAATGCGAGAGTACTGGCTCAAGCGCGGGATCTCGCTAGCGCTGTGTGGCCAAGTACTACATTGCATCGAGCTGATTCGCGCCGGTCACGTGCGAATTCGTTACCCGACAGGGTACAGACGGCTACATGCAAAAATGTACTGCTCCGCTGGAGCAGTGACGCTTGGGTCCAGCAACTACACTGGACCAGGTT‑TACATACTCAGCATGAAGCGAACGTGCGCTTCACCTCTGGGGAAAGAGCGCGCTTTGTTGAGACCAATCTCCTAGCTGAGTGCTTTTGGAGCTTGGG  >  NC_002947/2189428‑2189717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑