BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,287,681	T→A	8.9%	Y270N (TAC→AAC)	PP_2016 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,287,681	0	T	A	8.9%	346.0 / 16.6	120	Y270N (TAC→AAC)	PP_2016	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (57/52); new base A (6/5); total (63/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 122 total aligned reads displayed.

ATGAACTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTT  >  NC_002947/2287534‑2287828

aTGAACTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTa                                                                                                                                                    >  4:92352/1‑149 (MQ=255)

aTGAACTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTa                                                                                                                                                    >  3:29832/1‑149 (MQ=255)

aTGAACTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTa                                                                                                                                                    >  3:152432/1‑149 (MQ=255)

 tGAACTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGCCACGCTGCTGGGCATCAAGGGCCACTACCTGATCTTCGACCCCGGT‑GTGATCAATATTCGCAAGGACACGGCCTAc                                                                                                                                                   >  8:64380/1‑149 (MQ=255)

   aaCTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTAc                                                                                                                                                   >  5:274709/1‑147 (MQ=255)

    aCTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAg                                                                                                                                                <  6:110246/149‑1 (MQ=255)

     cTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGt                                                                                                                                               >  1:337184/1‑149 (MQ=255)

      tACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAActgg                                                                                                                                                >  7:2523/1‑144 (MQ=255)

        cccGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTgg                                                                                                                                            <  5:183721/149‑1 (MQ=255)

             tCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTg                                                                                                                                       <  8:2523/149‑1 (MQ=255)

              cGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgt                                                                                                                                      <  1:255802/149‑2 (MQ=255)

               gAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTTGCTGATCTTCGACAGCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgta                                                                                                                                     <  1:76517/149‑3 (MQ=255)

               gAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgta                                                                                                                                     <  5:20745/149‑3 (MQ=255)

                  gCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCa                                                                                                                                  <  2:337184/149‑1 (MQ=255)

                   cGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAg                                                                                                                                 <  1:254170/149‑1 (MQ=255)

                    gTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGt                                                                                                                                >  4:271162/1‑149 (MQ=255)

                     tACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGt                                                                                                                                >  5:294571/1‑148 (MQ=255)

                        ccGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGACACGCATCAGGGCATAAAGGGCCAGTAACTGATATTACACACCGGT‑GTGATAAATATTAGCAAGTACACCGCCAAACAGTTGGCCGTGCTACAGTaaa                                                                                                                             >  1:86392/1‑148 (MQ=255)

                         cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                           <  1:47067/149‑1 (MQ=255)

                         cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                           <  1:148042/149‑1 (MQ=255)

                         cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                           <  3:271162/149‑1 (MQ=255)

                          gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                           <  8:42018/148‑1 (MQ=255)

                          gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaa                                                                                                                             >  7:42018/1‑146 (MQ=255)

                          gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAg                                                                                                                          >  3:141040/1‑149 (MQ=255)

                             gaagaTTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAACGAc                                                                                                                       >  6:313882/1‑149 (MQ=255)

                               agaTTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCt                                                                                                                     <  6:274709/149‑1 (MQ=255)

                                     tCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTCGCCGTGCTCCAGTAAAAAGGACCTGCACAt                                                                                                               >  2:127620/1‑149 (MQ=255)

                                       aGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaa                                                                                                                            <  2:363035/134‑1 (MQ=255)

                                       aGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaa                                                                                                                            >  1:363035/1‑134 (MQ=255)

                                         cTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGt                                                                                                           <  3:92352/149‑1 (MQ=255)

                                            cAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTAc                                                                                                         <  8:352220/148‑1 (MQ=255)

                                               ccTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAGGGACCTGCACATGCGTACCGaa                                                                                                     >  6:56065/1‑149 (MQ=255)

                                                 tCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaac                                                                                                    >  1:58839/1‑148 (MQ=255)

                                                   gACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTAGCAAGTACACGGCCTACAAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGacaaac                                                                                                 >  6:268407/1‑144 (MQ=255)

                                                     cAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCg                                                                                                       >  8:284560/1‑141 (MQ=255)

                                                     cAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCg                                                                                                       <  7:284560/141‑1 (MQ=255)

                                                         gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAg                                                                                           <  5:319934/149‑1 (MQ=255)

                                                           cccGGTGGCCGAGGGCACGCTGCTGGACATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTg                                                                                         <  4:248339/149‑1 (MQ=255)

                                                             cGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGa                                                                                        <  2:174481/148‑1 (MQ=255)

                                                                  gCCGAGGGCAGGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACGAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTAc                                                                                   <  3:338856/148‑1 (MQ=255)

                                                                    cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                           <  2:41551/106‑1 (MQ=255)

                                                                    cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCaa                                                                                            >  1:109060/1‑137 (MQ=255)

                                                                       gggCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                             >  3:348873/1‑149 (MQ=255)

                                                                        ggCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgn                                                                            <  4:348873/149‑2 (MQ=255)

                                                                          cACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGAt                                                                          >  8:203840/1‑149 (MQ=255)

                                                                          cACGCTGCTCCGCAGCCACGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGAt                                                                          <  6:294571/149‑1 (MQ=255)

                                                                           aCGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaacaac                                                                                                 >  4:209089/1‑125 (MQ=255)

                                                                             gctgctGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTAc                                                                       <  4:29832/149‑1 (MQ=255)

                                                                                      cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAg                                                                    <  6:144031/143‑1 (MQ=255)

                                                                                      cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAg                                                                    >  5:144031/1‑143 (MQ=255)

                                                                                      cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                              >  8:157377/1‑149 (MQ=255)

                                                                                      cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                              >  2:83549/1‑149 (MQ=255)

                                                                                       aTCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACAGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                              >  8:46428/1‑148 (MQ=255)

                                                                                        tCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGa                                                            <  7:157377/149‑1 (MQ=255)

                                                                                          aaGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAg                                                           >  5:371138/1‑148 (MQ=255)

                                                                                            gggCCAGTACCTGATCTTCGACACCGGTAGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTa                                                         >  1:211210/1‑149 (MQ=255)

                                                                                              gCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACTGGGGGCCGGAGTACCt                                                      >  1:127444/1‑149 (MQ=255)

                                                                                                cAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGa                                                    <  5:268407/149‑1 (MQ=255)

                                                                                                   tACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATc                                                  >  4:23943/1‑148 (MQ=255)

                                                                                                    aCCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcga                                                >  4:321442/1‑149 (MQ=255)

                                                                                                      cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGa                                                    <  8:204140/143‑1 (MQ=255)

                                                                                                      cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                               <  6:132007/148‑1 (MQ=255)

                                                                                                      cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                               >  5:132007/1‑148 (MQ=255)

                                                                                                        gATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                               >  2:175008/1‑146 (MQ=255)

                                                                                                         aTCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgacga                                             <  3:54946/147‑1 (MQ=255)

                                                                                                             tCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCa                                       >  5:55446/1‑149 (MQ=255)

                                                                                                                            aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTTCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGc                                        <  4:44181/134‑1 (MQ=255)

                                                                                                                            aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGc                                        >  3:44181/1‑134 (MQ=255)

                                                                                                                            aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGc                         >  8:37905/1‑149 (MQ=255)

                                                                                                                              cAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTg                       >  7:333983/1‑149 (MQ=255)

                                                                                                                              cAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTg                       >  3:149230/1‑149 (MQ=255)

                                                                                                                               aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTGCCTGATCGACGAGACGCACCTGACCTTCGAGCTg                       <  5:313882/148‑1 (MQ=255)

                                                                                                                               aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGt                      <  4:82029/149‑1 (MQ=255)

                                                                                                                               aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGt                      <  8:333983/149‑1 (MQ=255)

                                                                                                                                atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTc                             <  6:184479/141‑1 (MQ=255)

                                                                                                                                atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGtt                     >  3:320173/1‑149 (MQ=255)

                                                                                                                                atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGtt                     >  5:66522/1‑149 (MQ=255)

                                                                                                                                atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGtt                     <  4:141040/149‑1 (MQ=255)

                                                                                                                                atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGCCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGtt                     >  7:48447/1‑149 (MQ=255)

                                                                                                                                     cGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAg                 <  2:211210/148‑1 (MQ=255)

                                                                                                                                      gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGc                                                                                                             <  8:208148/55‑1 (MQ=255)

                                                                                                                                      gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGc                                                                                                             >  7:208148/1‑55 (MQ=255)

                                                                                                                                      gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                             <  3:161511/87‑1 (MQ=255)

                                                                                                                                      gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                             >  4:161511/1‑87 (MQ=255)

                                                                                                                                      gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGa               <  1:129122/149‑1 (MQ=255)

                                                                                                                                        tggagcacGGCCAACTGGTAGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGAc              >  3:350456/6‑148 (MQ=255)

                                                                                                                                         aGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                               <  4:254330/114‑1 (MQ=255)

                                                                                                                                         aGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                               >  3:254330/1‑114 (MQ=255)

                                                                                                                                         aGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACca            >  6:314402/1‑149 (MQ=255)

                                                                                                                                          gTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACAGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACcac           >  6:272572/1‑149 (MQ=255)

                                                                                                                                            acacGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCacac         >  8:318933/1‑149 (MQ=255)

                                                                                                                                              acGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGc       <  5:29395/149‑1 (MQ=255)

                                                                                                                                              acGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGc       >  3:126694/1‑149 (MQ=255)

                                                                                                                                              acGGCCAACTGGTAGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGa                                  >  2:163227/1‑122 (MQ=255)

                                                                                                                                              acGGCCAACTGGTAGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGa                                  <  1:163227/122‑1 (MQ=255)

                                                                                                                                                ggCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCAACTGACCTTCGAGCTGTTCGAGGACCACACGCTg     >  4:139397/1‑149 (MQ=255)

                                                                                                                                                  ccTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGt   <  6:371138/149‑1 (MQ=255)

                                                                                                                                                  ccTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGt   <  7:46428/149‑1 (MQ=255)

                                                                                                                                                    tACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCt      <  7:65882/144‑1 (MQ=255)

                                                                                                                                                    tACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCt      >  8:65882/1‑144 (MQ=255)

ATGAACTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTT  >  NC_002947/2287534‑2287828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`