BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,287,685	A→G	7.5%	Q271R (CAG→CGG) ‡	PP_2016 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,287,685	0	A	G	7.5%	348.4 / 13.3	120	Q271R (CAG→CGG) ‡	PP_2016	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (56/55); new base G (4/5); total (60/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 120 total aligned reads displayed.

ACTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTCACG  >  NC_002947/2287538‑2287832

aCTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAg                                                                                                                                                    <  6:110246/149‑1 (MQ=255)

 cTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGt                                                                                                                                                   >  1:337184/1‑149 (MQ=255)

    cccGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTgg                                                                                                                                                <  5:183721/149‑1 (MQ=255)

         tCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTg                                                                                                                                           <  8:2523/149‑1 (MQ=255)

          cGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgt                                                                                                                                          <  1:255802/149‑2 (MQ=255)

           gAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTTGCTGATCTTCGACAGCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgta                                                                                                                                         <  1:76517/149‑3 (MQ=255)

           gAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgta                                                                                                                                         <  5:20745/149‑3 (MQ=255)

              gCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCa                                                                                                                                      <  2:337184/149‑1 (MQ=255)

               cGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAg                                                                                                                                     <  1:254170/149‑1 (MQ=255)

                gTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGt                                                                                                                                    >  4:271162/1‑149 (MQ=255)

                 tACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGt                                                                                                                                    >  5:294571/1‑148 (MQ=255)

                    ccGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGACACGCATCAGGGCATAAAGGGCCAGTAACTGATATTACACACCGGT‑GTGATAAATATTAGCAAGTACACCGCCAAACAGTTGGCCGTGCTACAGTaaa                                                                                                                                 >  1:86392/1‑148 (MQ=255)

                     cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                               <  1:148042/149‑1 (MQ=255)

                     cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                               <  4:275670/149‑1 (MQ=255)

                     cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                               <  1:47067/149‑1 (MQ=255)

                     cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                               <  3:271162/149‑1 (MQ=255)

                      gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                               <  8:42018/148‑1 (MQ=255)

                      gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaa                                                                                                                                 >  7:42018/1‑146 (MQ=255)

                      gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAg                                                                                                                              >  3:141040/1‑149 (MQ=255)

                      gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCAGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACAAGTTGGCCGTGCTCCAGTAAAAAg                                                                                                                              >  4:54946/1‑149 (MQ=255)

                       aagaagATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATCCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAgg                                                                                                                             <  6:268126/149‑1 (MQ=255)

                           agaTTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCt                                                                                                                         <  6:274709/149‑1 (MQ=255)

                                 tCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTCGCCGTGCTCCAGTAAAAAGGACCTGCACAt                                                                                                                   >  2:127620/1‑149 (MQ=255)

                                   aGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaa                                                                                                                                <  2:363035/134‑1 (MQ=255)

                                     cTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGt                                                                                                               <  3:92352/149‑1 (MQ=255)

                                           ccTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAGGGACCTGCACATGCGTACCGaa                                                                                                         >  6:56065/1‑149 (MQ=255)

                                             tCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaac                                                                                                        >  1:58839/1‑148 (MQ=255)

                                                 cAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCg                                                                                                           >  8:284560/1‑141 (MQ=255)

                                                 cAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCg                                                                                                           <  7:284560/141‑1 (MQ=255)

                                                     gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGc                                                                                                                 >  7:40636/1‑131 (MQ=255)

                                                     gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGc                                                                                                                 <  8:40636/131‑1 (MQ=255)

                                                     gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAg                                                                                               <  5:319934/149‑1 (MQ=255)

                                                       cccGGTGGCCGAGGGCACGCTGCTGGACATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTg                                                                                             <  4:248339/149‑1 (MQ=255)

                                                         cGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGa                                                                                            <  2:174481/148‑1 (MQ=255)

                                                          ggTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATc                                                                                          <  8:27597/149‑1 (MQ=255)

                                                              gCCGAGGGCAGGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACGAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTAc                                                                                       <  3:338856/148‑1 (MQ=255)

                                                                cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                               >  1:41551/1‑106 (MQ=255)

                                                                cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                               <  2:41551/106‑1 (MQ=255)

                                                                cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCaa                                                                                                >  1:109060/1‑137 (MQ=255)

                                                                   gggCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                                 >  3:348873/1‑149 (MQ=255)

                                                                    ggCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgn                                                                                <  4:348873/149‑2 (MQ=255)

                                                                      cACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGAt                                                                              >  8:203840/1‑149 (MQ=255)

                                                                      cACGCTGCTCCGCAGCCACGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGAt                                                                              <  6:294571/149‑1 (MQ=255)

                                                                       aCGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaacaac                                                                                                     >  4:209089/1‑125 (MQ=255)

                                                                       aCGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaacaac                                                                                                     <  3:209089/125‑1 (MQ=255)

                                                                         gctgctGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTAc                                                                           <  4:29832/149‑1 (MQ=255)

                                                                                  cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAg                                                                        <  6:144031/143‑1 (MQ=255)

                                                                                  cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAg                                                                        >  5:144031/1‑143 (MQ=255)

                                                                                  cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                                  >  8:157377/1‑149 (MQ=255)

                                                                                  cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                                  >  2:83549/1‑149 (MQ=255)

                                                                                   aTCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACAGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                                  >  8:46428/1‑148 (MQ=255)

                                                                                    tCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGa                                                                <  7:157377/149‑1 (MQ=255)

                                                                                      aaGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAg                                                               >  5:371138/1‑148 (MQ=255)

                                                                                        gggCCAGTACCTGATCTTCGACACCGGTAGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTa                                                             >  1:211210/1‑149 (MQ=255)

                                                                                          gCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACTGGGGGCCGGAGTACCt                                                          >  1:127444/1‑149 (MQ=255)

                                                                                            cAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGa                                                        <  5:268407/149‑1 (MQ=255)

                                                                                               tACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATc                                                      >  4:23943/1‑148 (MQ=255)

                                                                                                aCCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcga                                                    >  4:321442/1‑149 (MQ=255)

                                                                                                 ccTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                   >  6:303676/1‑149 (MQ=255)

                                                                                                  cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGa                                                        <  8:204140/143‑1 (MQ=255)

                                                                                                  cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGa                                                        >  7:204140/1‑143 (MQ=255)

                                                                                                  cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                   >  5:132007/1‑148 (MQ=255)

                                                                                                  cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                   <  6:132007/148‑1 (MQ=255)

                                                                                                    gATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                   >  2:175008/1‑146 (MQ=255)

                                                                                                     aTCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgacga                                                 <  3:54946/147‑1 (MQ=255)

                                                                                                         tCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCa                                           >  5:55446/1‑149 (MQ=255)

                                                                                                          cGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCAc                                          >  3:82029/1‑149 (MQ=255)

                                                                                                                   t‑gtgATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAGGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTc                                 <  5:56065/149‑1 (MQ=255)

                                                                                                                        aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGc                                            >  3:44181/1‑134 (MQ=255)

                                                                                                                        aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGc                             >  8:37905/1‑149 (MQ=255)

                                                                                                                         tCAATATTCGCAAGTACACGGCCTACCAGTTGGCAGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCt                            >  4:207205/1‑149 (MQ=255)

                                                                                                                          cAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTg                           >  3:149230/1‑149 (MQ=255)

                                                                                                                          cAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTg                           >  7:333983/1‑149 (MQ=255)

                                                                                                                           aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTGCCTGATCGACGAGACGCACCTGACCTTCGAGCTg                           <  5:313882/148‑1 (MQ=255)

                                                                                                                           aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGt                          <  8:333983/149‑1 (MQ=255)

                                                                                                                           aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGt                          <  4:82029/149‑1 (MQ=255)

                                                                                                                            atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTc                                 <  6:184479/141‑1 (MQ=255)

                                                                                                                            atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGtt                         >  3:320173/1‑149 (MQ=255)

                                                                                                                               ttCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGa                      <  1:175008/149‑1 (MQ=255)

                                                                                                                                 cGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAg                     <  2:211210/148‑1 (MQ=255)

                                                                                                                                  gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                                 >  4:161511/1‑87 (MQ=255)

                                                                                                                                  gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                                 <  3:161511/87‑1 (MQ=255)

                                                                                                                                  gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGa                   <  1:129122/149‑1 (MQ=255)

                                                                                                                                  gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGCTCGAGGa                   <  3:321442/149‑1 (MQ=255)

                                                                                                                                     aGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACca                >  6:314402/1‑149 (MQ=255)

                                                                                                                                      gTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACAGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACcac               >  6:272572/1‑149 (MQ=255)

                                                                                                                                        acacGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCacac             >  8:318933/1‑149 (MQ=255)

                                                                                                                                          acGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGc           >  3:126694/1‑149 (MQ=255)

                                                                                                                                          acGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGc           <  5:29395/149‑1 (MQ=255)

                                                                                                                                          acGGCCAACTGGTAGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGa                                      >  2:163227/1‑122 (MQ=255)

                                                                                                                                            ggCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCAACTGACCTTCGAGCTGTTCGAGGACCACACGCTg         >  4:139397/1‑149 (MQ=255)

                                                                                                                                              ccTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGt       <  7:46428/149‑1 (MQ=255)

                                                                                                                                              ccTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGt       <  6:371138/149‑1 (MQ=255)

                                                                                                                                               cTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGtt      >  8:196515/1‑149 (MQ=255)

                                                                                                                                                tACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCt          <  7:65882/144‑1 (MQ=255)

                                                                                                                                                tACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCt          >  8:65882/1‑144 (MQ=255)

                                                                                                                                                 aCCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgg                                                                                <  5:298403/73‑1 (MQ=255)

                                                                                                                                                 aCCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgg                                                                                >  6:298403/1‑73 (MQ=255)

                                                                                                                                                  ccAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTc     <  2:58839/147‑1 (MQ=255)

                                                                                                                                                   cAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTCAcg  >  1:142625/1‑149 (MQ=255)

ACTACCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTCACG  >  NC_002947/2287538‑2287832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`