BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,287,688	T→A	7.5%	L272* (TTG→TAG)	PP_2016 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,287,688	0	T	A	7.5%	363.9 / 11.6	119	L272* (TTG→TAG)	PP_2016	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (55/55); new base A (4/5); total (59/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTCACGCG  >  NC_002947/2287542‑2287834

cccGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTgg                                                                                                                                                  <  5:183721/149‑1 (MQ=255)

     tCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTg                                                                                                                                             <  8:2523/149‑1 (MQ=255)

      cGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgt                                                                                                                                            <  1:255802/149‑2 (MQ=255)

       gAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTTGCTGATCTTCGACAGCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgta                                                                                                                                           <  1:76517/149‑3 (MQ=255)

       gAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCCGTgta                                                                                                                                           <  5:20745/149‑3 (MQ=255)

          gCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCa                                                                                                                                        <  2:337184/149‑1 (MQ=255)

           cGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAg                                                                                                                                       <  1:254170/149‑1 (MQ=255)

            gTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGt                                                                                                                                      >  4:271162/1‑149 (MQ=255)

                ccGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGACACGCATCAGGGCATAAAGGGCCAGTAACTGATATTACACACCGGT‑GTGATAAATATTAGCAAGTACACCGCCAAACAGTTGGCCGTGCTACAGTaaa                                                                                                                                   >  1:86392/1‑148 (MQ=255)

                 cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                                 <  1:148042/149‑1 (MQ=255)

                 cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                                 <  4:275670/149‑1 (MQ=255)

                 cGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                                 <  3:271162/149‑1 (MQ=255)

                  gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                                 <  8:42018/148‑1 (MQ=255)

                  gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaa                                                                                                                                   >  7:42018/1‑146 (MQ=255)

                  gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAg                                                                                                                                >  3:141040/1‑149 (MQ=255)

                  gaagaaGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCAGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACAAGTTGGCCGTGCTCCAGTAAAAAg                                                                                                                                >  4:54946/1‑149 (MQ=255)

                   aagaagATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATCCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAgg                                                                                                                               <  6:268126/149‑1 (MQ=255)

                       agaTTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCt                                                                                                                           <  6:274709/149‑1 (MQ=255)

                             tCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTCGCCGTGCTCCAGTAAAAAGGACCTGCACAt                                                                                                                     >  2:127620/1‑149 (MQ=255)

                               aGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaa                                                                                                                                  <  2:363035/134‑1 (MQ=255)

                               aGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaa                                                                                                                                  >  1:363035/1‑134 (MQ=255)

                                 cTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGt                                                                                                                 <  3:92352/149‑1 (MQ=255)

                                       ccTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAGGGACCTGCACATGCGTACCGaa                                                                                                           >  6:56065/1‑149 (MQ=255)

                                         tCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaac                                                                                                          >  1:58839/1‑148 (MQ=255)

                                             cAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCg                                                                                                             >  8:284560/1‑141 (MQ=255)

                                             cAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCg                                                                                                             <  7:284560/141‑1 (MQ=255)

                                                 gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGc                                                                                                                   <  8:40636/131‑1 (MQ=255)

                                                 gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGc                                                                                                                   >  7:40636/1‑131 (MQ=255)

                                                 gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAg                                                                                                 <  5:319934/149‑1 (MQ=255)

                                                   cccGGTGGCCGAGGGCACGCTGCTGGACATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTg                                                                                               <  4:248339/149‑1 (MQ=255)

                                                     cGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGa                                                                                              <  2:174481/148‑1 (MQ=255)

                                                      ggTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATc                                                                                            <  8:27597/149‑1 (MQ=255)

                                                          gCCGAGGGCAGGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACGAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTAc                                                                                         <  3:338856/148‑1 (MQ=255)

                                                            cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTaaaaa                                                                                                                                 >  1:41551/1‑106 (MQ=255)

                                                            cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCaa                                                                                                  >  1:109060/1‑137 (MQ=255)

                                                            cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCaa                                                                                                  <  2:109060/137‑1 (MQ=255)

                                                               gggCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                                   >  3:348873/1‑149 (MQ=255)

                                                                ggCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgn                                                                                  <  4:348873/149‑2 (MQ=255)

                                                                  cACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGAt                                                                                >  8:203840/1‑149 (MQ=255)

                                                                  cACGCTGCTCCGCAGCCACGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGAt                                                                                <  6:294571/149‑1 (MQ=255)

                                                                   aCGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaacaac                                                                                                       >  4:209089/1‑125 (MQ=255)

                                                                   aCGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaacaac                                                                                                       <  3:209089/125‑1 (MQ=255)

                                                                     gctgctGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTAc                                                                             <  4:29832/149‑1 (MQ=255)

                                                                         ctgGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAg                                                                          <  2:86392/148‑1 (MQ=255)

                                                                              cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAg                                                                          <  6:144031/143‑1 (MQ=255)

                                                                              cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                                    >  8:157377/1‑149 (MQ=255)

                                                                              cATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                                    >  2:83549/1‑149 (MQ=255)

                                                                               aTCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACAGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                                    >  8:46428/1‑148 (MQ=255)

                                                                                tCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGa                                                                  <  7:157377/149‑1 (MQ=255)

                                                                                  aaGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAg                                                                 >  5:371138/1‑148 (MQ=255)

                                                                                    gggCCAGTACCTGATCTTCGACACCGGTAGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTa                                                               >  1:211210/1‑149 (MQ=255)

                                                                                      gCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACTGGGGGCCGGAGTACCt                                                            >  1:127444/1‑149 (MQ=255)

                                                                                        cAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGa                                                          <  5:268407/149‑1 (MQ=255)

                                                                                             ccTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                     >  6:303676/1‑149 (MQ=255)

                                                                                             ccTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                     <  5:303676/149‑1 (MQ=255)

                                                                                              cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGa                                                          <  8:204140/143‑1 (MQ=255)

                                                                                              cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                     >  5:132007/1‑148 (MQ=255)

                                                                                              cTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                     <  6:132007/148‑1 (MQ=255)

                                                                                                gATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                     >  2:175008/1‑146 (MQ=255)

                                                                                                 aTCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgacga                                                   <  3:54946/147‑1 (MQ=255)

                                                                                                     tCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCa                                             >  5:55446/1‑149 (MQ=255)

                                                                                                                    aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTTCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGc                                              <  4:44181/134‑1 (MQ=255)

                                                                                                                    aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGc                                              >  3:44181/1‑134 (MQ=255)

                                                                                                                    aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGc                               >  8:37905/1‑149 (MQ=255)

                                                                                                                     tCAATATTCGCAAGTACACGGCCTACCAGTTGGCAGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCt                              >  4:207205/1‑149 (MQ=255)

                                                                                                                      cAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTg                             >  7:333983/1‑149 (MQ=255)

                                                                                                                       aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTGCCTGATCGACGAGACGCACCTGACCTTCGAGCTg                             <  5:313882/148‑1 (MQ=255)

                                                                                                                       aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGt                            <  8:333983/149‑1 (MQ=255)

                                                                                                                       aaTATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGt                            <  4:82029/149‑1 (MQ=255)

                                                                                                                        atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTc                                   <  6:184479/141‑1 (MQ=255)

                                                                                                                        atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTc                                   >  5:184479/1‑141 (MQ=255)

                                                                                                                        atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGtt                           <  4:141040/149‑1 (MQ=255)

                                                                                                                        atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGtt                           >  3:320173/1‑149 (MQ=255)

                                                                                                                        atatTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGtt                           >  5:66522/1‑149 (MQ=255)

                                                                                                                             cGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAg                       <  2:211210/148‑1 (MQ=255)

                                                                                                                              gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGc                                                                                                                   >  7:208148/1‑55 (MQ=255)

                                                                                                                              gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                                   <  3:161511/87‑1 (MQ=255)

                                                                                                                              gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAg                                                                                   >  4:161511/1‑87 (MQ=255)

                                                                                                                              gCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGa                     <  1:129122/149‑1 (MQ=255)

                                                                                                                                tggagcacGGCCAACTGGTAGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGAc                    >  3:350456/6‑148 (MQ=255)

                                                                                                                                 aGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                     <  4:254330/114‑1 (MQ=255)

                                                                                                                                 aGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                                     >  3:254330/1‑114 (MQ=255)

                                                                                                                                 aGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACca                  >  6:314402/1‑149 (MQ=255)

                                                                                                                                  gTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACAGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACcac                 >  6:272572/1‑149 (MQ=255)

                                                                                                                                    acacGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCacac               >  8:318933/1‑149 (MQ=255)

                                                                                                                                     cacGGCCAACTGGTAGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACg              >  2:261831/1‑149 (MQ=255)

                                                                                                                                      acGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGc             >  3:126694/1‑149 (MQ=255)

                                                                                                                                      acGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGc             <  5:29395/149‑1 (MQ=255)

                                                                                                                                      acGGCCAACTGGTAGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGa                                        <  1:163227/122‑1 (MQ=255)

                                                                                                                                        ggCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCAACTGACCTTCGAGCTGTTCGAGGACCACACGCTg           >  4:139397/1‑149 (MQ=255)

                                                                                                                                          ccTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGt         <  7:46428/149‑1 (MQ=255)

                                                                                                                                          ccTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGt         <  6:371138/149‑1 (MQ=255)

                                                                                                                                           cTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGtt        >  8:196515/1‑149 (MQ=255)

                                                                                                                                            tACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCt            <  7:65882/144‑1 (MQ=255)

                                                                                                                                            tACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCt            >  8:65882/1‑144 (MQ=255)

                                                                                                                                             aCCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgg                                                                                  <  5:298403/73‑1 (MQ=255)

                                                                                                                                             aCCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgg                                                                                  >  6:298403/1‑73 (MQ=255)

                                                                                                                                              ccAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTc       <  2:58839/147‑1 (MQ=255)

                                                                                                                                               cAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTCAcg    >  1:142625/1‑149 (MQ=255)

                                                                                                                                                 gTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACATGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTCAcgcg  <  7:203840/149‑1 (MQ=255)

CCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGT‑GTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCCGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTTCACGCG  >  NC_002947/2287542‑2287834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`