BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,311,049	T→C	5.9%	V267A (GTA→GCA) ‡	PP_2033 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,311,049	0	T	C	5.9%	303.5 / 6.4	101	V267A (GTA→GCA) ‡	PP_2033	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (53/42); new base C (2/4); total (55/46)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 107 total aligned reads displayed.

ACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAG  >  NC_002947/2310905‑2311197

aCGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGTGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGc                                                                                                                                                   <  7:280342/149‑1 (MQ=255)

        cggcATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACA‑GGCAcgcactg                                                                                                                                           <  6:115404/149‑5 (MQ=255)

         ggcATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGCGCGTGCCTACA‑GGCAcgcactgg                                                                                                                                          <  2:157356/149‑6 (MQ=255)

              aaCTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAg                                                                                                                                       >  7:143073/1‑147 (MQ=255)

               aCTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCa                                                                                                                                     <  3:268791/148‑1 (MQ=255)

                cTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCAc                                                                                                                                                 >  8:307358/1‑135 (MQ=255)

                cTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCAc                                                                                                                                                 <  7:307358/135‑1 (MQ=255)

                 ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACCTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGt                                                                                                                                   >  1:273805/1‑149 (MQ=255)

                 ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTg                                                                                                                                  >  5:347461/1‑149 (MQ=255)

                 ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACA‑GGCAcgcactggg                                                                                                                                         <  3:154201/142‑7 (MQ=255)

                       cTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGt                                                                                                                            <  5:357617/149‑1 (MQ=255)

                        tGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGATTATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCGAGTGCGTGCCTGTA‑GGCACGCGTGTc                                                                                                                                          <  5:75069/134‑1 (MQ=255)

                        tGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTc                                                                                                                                          >  6:75069/1‑134 (MQ=255)

                        tGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAg                                                                                                                                       >  1:80178/1‑137 (MQ=255)

                           tGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑At                                                                                                                       <  6:347461/149‑1 (MQ=255)

                             cgcAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGa                                                                                                                                        >  3:192729/1‑131 (MQ=255)

                             cgcAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGa                                                                                                                                        <  4:192729/131‑1 (MQ=255)

                                    gacACTACTTCGTGCTGCCGGTGGTGCA‑AGCGAATATTGGGGGGTGTTACCCGGAGAGCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCTTGTCGAGCAGTGCCGCGGAC‑ATGCCAAGGt                                                                                                               <  3:209321/146‑1 (MQ=255)

                                     ccACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTATAGCACGCGTGTCGAGCAGTGCGGCGTAC‑ATGCCAAGGt                                                                                                               >  1:123751/1‑148 (MQ=255)

                                      cACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGCGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAg                                                                                                            >  7:331354/1‑149 (MQ=255)

                                       aCGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGt                                                                                                               >  8:187240/1‑145 (MQ=255)

                                       aCGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGt                                                                                                               <  7:187240/145‑1 (MQ=255)

                                       aCGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGt                                                                                                           >  2:114641/1‑149 (MQ=255)

                                       aCGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGt                                                                                                           <  7:283457/149‑1 (MQ=255)

                                              cGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAc                                                                                                                          <  2:269457/128‑1 (MQ=255)

                                              cGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAc                                                                                                                          >  1:269457/1‑128 (MQ=255)

                                               gTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGTACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGc                                                                                                    <  1:121973/148‑1 (MQ=255)

                                               gTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCt                                                                                                   >  4:309130/1‑149 (MQ=255)

                                               gTGCTGACGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTNCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGTACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGc                                                                                                    <  1:117719/148‑1 (MQ=255)

                                                    gcCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCtt                                                                                              >  6:331515/1‑149 (MQ=255)

                                                     ccGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCtt                                                                                              <  3:213609/148‑1 (MQ=255)

                                                           gTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGtt                                                                                       <  3:309130/149‑1 (MQ=255)

                                                                 cTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGcc                                                                                   >  3:14370/1‑148 (MQ=255)

                                                                 cTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ACGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCg                                                                                  <  2:273805/149‑1 (MQ=255)

                                                                  tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAgg                                                                                                                >  6:312892/1‑118 (MQ=255)

                                                                  tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAgg                                                                                                                <  5:312892/118‑1 (MQ=255)

                                                                  tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTg                                                                                                  <  2:39764/132‑1 (MQ=255)

                                                                  tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                                 >  3:254312/1‑149 (MQ=255)

                                                                  tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                                 >  1:36494/1‑149 (MQ=255)

                                                                       tatTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATgg                                                                            <  5:159603/149‑1 (MQ=255)

                                                                         ttGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATggg                                                                           <  5:102508/148‑1 (MQ=255)

                                                                           gggggTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCg                                                                         <  3:318263/148‑1 (MQ=255)

                                                                           gggggTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCg                                                                         >  4:287033/1‑148 (MQ=255)

                                                                                      cccGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgc                                                             >  7:169187/1‑149 (MQ=255)

                                                                                       ccGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcg                                                            >  7:24922/1‑149 (MQ=255)

                                                                                        cGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCcgcgc                                                           <  2:36494/149‑1 (MQ=255)

                                                                                             aCCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgat                                                      <  8:204623/149‑1 (MQ=255)

                                                                                               ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                    >  4:254346/1‑149 (MQ=255)

                                                                                               ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                    >  6:182043/1‑149 (MQ=255)

                                                                                               ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                    >  7:99094/1‑149 (MQ=255)

                                                                                               ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                    >  7:101389/1‑149 (MQ=255)

                                                                                               ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGACTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                    >  1:367544/1‑149 (MQ=255)

                                                                                                      gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTgg                                               <  8:24922/147‑1 (MQ=255)

                                                                                                      gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGgga                                             <  3:287033/149‑1 (MQ=255)

                                                                                                       aGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGggag                                            <  8:143073/149‑1 (MQ=255)

                                                                                                           ggTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                        >  1:89828/1‑149 (MQ=255)

                                                                                                           ggTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGACGCGCGTGATGACCTGGGAGGAGc                                        >  7:356355/1‑149 (MQ=255)

                                                                                                            gtgtTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGcc                                       >  1:151664/1‑149 (MQ=255)

                                                                                                              gtTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGa                                     >  5:192791/1‑149 (MQ=255)

                                                                                                                 gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatg                                                     >  8:105979/1‑130 (MQ=255)

                                                                                                                 gCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                   <  4:14370/148‑1 (MQ=255)

                                                                                                                  cTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCGTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGc                                 >  5:263281/1‑149 (MQ=255)

                                                                                                                       cAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGt                                                         >  7:165423/1‑120 (MQ=255)

                                                                                                                       cAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGt                                                         <  8:165423/120‑1 (MQ=255)

                                                                                                                       cAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                            >  1:16437/1‑149 (MQ=255)

                                                                                                                       cAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                            >  8:228900/1‑149 (MQ=255)

                                                                                                                       cAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GCCACGCGTCTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATCACCTCGGAGGAGCCGATTGCCGGTg                            >  8:223182/1‑149 (MQ=255)

                                                                                                                        aaGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTgg                           >  3:86707/1‑149 (MQ=255)

                                                                                                                          gCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggc                         >  8:100449/1‑149 (MQ=255)

                                                                                                                           ccGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcg                        >  5:294659/1‑149 (MQ=255)

                                                                                                                             gCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGACGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcggc                      >  1:227446/1‑149 (MQ=255)

                                                                                                                               aGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGGCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGcggcgg                    <  4:86707/149‑1 (MQ=255)

                                                                                                                                 tCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGa                  <  2:89828/149‑1 (MQ=255)

                                                                                                                                  cgacacgcgtgcCTACA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc                 >  4:59824/7‑149 (MQ=255)

                                                                                                                                   gacacgcgtgcCTACA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                        <  2:164701/120‑1 (MQ=255)

                                                                                                                                   ccAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc                 >  6:168355/1‑148 (MQ=255)

                                                                                                                                   ccAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc                 >  3:349250/1‑148 (MQ=255)

                                                                                                                                   ccAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc                 >  6:64154/1‑148 (MQ=255)

                                                                                                                                    ctgtgcgtgcCTGTA‑GGCTCGCGTGTGGTTCAGTTCCGCGTAG‑ATGCAAATGTGTGTGCCTTGCTGTCTTCGTCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGTCATGGGAGGAGCGGATTGCCGGTGGGCGGCGGGACGa               <  2:367544/147‑1 (MQ=255)

                                                                                                                                    cAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTACGATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGTTGACCTGGGAGGAGCCGATTGCGGGTGGGCGGCgagagc                >  1:70976/1‑147 (MQ=255)

                                                                                                                                    cAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGc                                                                                                    <  7:223504/64‑1 (MQ=255)

                                                                                                                                    cAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGc                                                                                                    >  8:223504/1‑64 (MQ=255)

                                                                                                                                    cAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcggc                      <  2:70976/142‑1 (MQ=255)

                                                                                                                                    cAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGa               <  2:123751/149‑1 (MQ=255)

                                                                                                                                       ggcgtgcCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGgc            <  1:2495/148‑1 (MQ=255)

                                                                                                                                            gcCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAAc                                                                      >  4:54201/1‑86 (MQ=255)

                                                                                                                                            gcCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCaa        >  1:225657/1‑148 (MQ=255)

                                                                                                                                             ccTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATTTGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCaaaa      <  1:114641/149‑1 (MQ=255)

                                                                                                                                              cTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGggagga                                          >  7:334379/1‑112 (MQ=255)

                                                                                                                                              cTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGggagga                                          <  8:334379/112‑1 (MQ=255)

                                                                                                                                              cTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                   <  6:248423/119‑1 (MQ=255)

                                                                                                                                              cTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                   >  5:248423/1‑119 (MQ=255)

                                                                                                                                              cTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                   <  2:344471/119‑1 (MQ=255)

                                                                                                                                              cTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAtt                                   >  1:344471/1‑119 (MQ=255)

                                                                                                                                                gTA‑GGCCCGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGAAATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAg     >  1:114503/1‑147 (MQ=255)

                                                                                                                                                 tA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCATGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGTTGACCTGGGAGGAGCCGATTGCCGGTGGGGGGCGGGACGAGGCGCAAAAGCAg  <  7:223182/149‑1 (MQ=255)

                                                                                                                                                 tA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCa   <  8:169187/148‑1 (MQ=255)

                                                                                                                                                 tA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCa   <  5:64154/148‑1 (MQ=255)

                                                                                                                                                 tA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAg  <  7:228900/149‑1 (MQ=255)

                                                                                                                                                 tA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAg  <  4:254312/149‑1 (MQ=255)

ACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCA‑CTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTA‑GGCACGCGTGTCGAGCAGTGCCGCGTAC‑ATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAG  >  NC_002947/2310905‑2311197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`