BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,329,848	C→T	5.5%	G67G (GGC→GGT)	PP_2048 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,329,848	0	C	T	5.5%	383.7 / 6.4	124	G67G (GGC→GGT)	PP_2048	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (58/59); new base T (5/2); total (63/61)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 124 total aligned reads displayed.

ACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGATGGCG  >  NC_002947/2329703‑2329995

aCCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGt                                                                                                                                                  >  5:275162/1‑149 (MQ=255)

 ccTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGt                                                                                                                                                  >  5:40657/1‑148 (MQ=255)

  cTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGa                                                                                                                                                <  3:188518/149‑1 (MQ=255)

      gCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGACCAGGGCTGTCAGTGGGTGGGCGGTGAGGTc                                                                                                                                            >  2:269437/1‑149 (MQ=255)

        ggACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTcac                                                                                                                                          <  6:40657/149‑1 (MQ=255)

        ggACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTcac                                                                                                                                          <  7:79537/149‑1 (MQ=255)

         gACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTcacc                                                                                                                                         <  2:151992/149‑1 (MQ=255)

          aCATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTCAGGTc                                                                                                                                            >  5:224689/1‑145 (MQ=255)

            aTTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCaccacc                                                                                                                                      >  6:164957/1‑149 (MQ=255)

             ttGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCAccc                                                                                                                                     <  2:262254/149‑1 (MQ=255)

                 cAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGa                                                                                                                                 >  2:13103/1‑149 (MQ=255)

                   gCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACg                                                                                                                               <  1:291191/149‑1 (MQ=255)

                     aaCTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACgg                                                                                                                              >  1:358975/1‑148 (MQ=255)

                       cTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCAt                                                                                                                           >  5:184903/1‑149 (MQ=255)

                          ggTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCTTCAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCg                                                                                                                        <  1:292817/149‑1 (MQ=255)

                          ggTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATg                                                                                                                          <  1:13103/147‑1 (MQ=255)

                                 aaCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGcc                                                                                                                 >  5:367845/1‑149 (MQ=255)

                                  aCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCgg                                                                                                                       <  7:134860/142‑1 (MQ=255)

                                  aCTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCgg                                                                                                                       >  8:134860/1‑142 (MQ=255)

                                    tGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTAt                                                                                                              >  8:327853/1‑149 (MQ=255)

                                       cacgcgACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCgg                                                                                                           <  1:316949/147‑1 (MQ=255)

                                        ccGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCgg                                                                                                           >  2:316949/1‑148 (MQ=255)

                                              cacCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACg                                                                                                    >  5:121327/1‑149 (MQ=255)

                                               acCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGAc                                                                                                                    >  2:314583/1‑132 (MQ=255)

                                               acCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGAc                                                                                                                    <  1:314583/132‑1 (MQ=255)

                                                cccTCGACGGCATGCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTc                                                                                                  <  6:304589/149‑1 (MQ=255)

                                                cccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTc                                                                                                  <  2:350006/149‑1 (MQ=255)

                                                 ccTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTc                                                                                                  >  3:88252/1‑148 (MQ=255)

                                                  cTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGTGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCg                                                                                                 >  2:296553/1‑148 (MQ=255)

                                                   tCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCaccacc                                                                                                                                      <  5:148894/110‑1 (MQ=255)

                                                   tCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCaccacc                                                                                                                                      >  6:148894/1‑110 (MQ=255)

                                                   tCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCgg                                                                                                                                  <  4:366239/114‑1 (MQ=255)

                                                   tCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCgg                                                                                                                                  >  3:366239/1‑114 (MQ=255)

                                                     gACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGaa                                                                                               <  6:275162/147‑1 (MQ=255)

                                                      aCGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACAt                                                                                            >  8:319817/1‑149 (MQ=255)

                                                       cGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATg                                                                                           <  2:358975/149‑1 (MQ=255)

                                                        ggCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTTACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACTGCATGCGGGACGCCTATCGGCAATACGTCGAACATgg                                                                                          <  2:235803/149‑1 (MQ=255)

                                                                 gaggagGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAg                                                                                 >  1:151581/1‑149 (MQ=255)

                                                                  aggaggCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGc                                                                                <  6:121327/149‑1 (MQ=255)

                                                                    gaggCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGc                                                                                    <  7:234340/143‑1 (MQ=255)

                                                                    gaggCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGc                                                                                    >  8:234340/1‑143 (MQ=255)

                                                                    gaggCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCAt                                                                              <  3:112923/149‑1 (MQ=255)

                                                                    gaggCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCAt                                                                              <  6:184903/149‑1 (MQ=255)

                                                                         cGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGTCCg                                                                         >  5:100415/1‑149 (MQ=255)

                                                                         cGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCg                                                                         >  6:178632/1‑149 (MQ=255)

                                                                          gCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCgg                                                                        >  6:6835/1‑149 (MQ=255)

                                                                            aCGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGc                                                                      <  7:267083/149‑1 (MQ=255)

                                                                               tttCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGc                                                                   <  5:164957/149‑1 (MQ=255)

                                                                                 tCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCa                                                                  <  5:178632/148‑1 (MQ=255)

                                                                                   aCTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGc                                                               >  7:224664/1‑149 (MQ=255)

                                                                                        ccAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGTGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGTCCGGGCCGCAGGCCTAtg                                                          <  6:100415/149‑1 (MQ=255)

                                                                                          aGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATc                                                                                                             >  7:100653/1‑96 (MQ=255)

                                                                                          aGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCg                                                                                                            >  4:92151/1‑97 (MQ=255)

                                                                                          aGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCg                                                                                                            <  3:92151/97‑1 (MQ=255)

                                                                                          aGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTAtggt                                                        >  1:335138/1‑149 (MQ=255)

                                                                                             tcatcGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCgg                                                                                                           >  4:116159/1‑95 (MQ=255)

                                                                                             tcatcGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCgg                                                                                                           <  3:116159/95‑1 (MQ=255)

                                                                                             tcatcGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCAGGCGGGACGCCTATCGGCAATACGGCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTggg                                                     <  7:89206/149‑1 (MQ=255)

                                                                                                tcGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGTCCGCAGGCCTATGGTGGGCAg                                                  >  6:302518/1‑149 (MQ=255)

                                                                                                  gCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTAt                                                           >  1:5534/1‑138 (MQ=255)

                                                                                                  gCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTAt                                                           <  2:5534/138‑1 (MQ=255)

                                                                                                  gCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAgg                                                 <  7:327853/148‑1 (MQ=255)

                                                                                                   cACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGt                                               <  8:224664/149‑1 (MQ=255)

                                                                                                     ccGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGc                                                    >  8:127353/1‑142 (MQ=255)

                                                                                                     ccGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCt                                             <  5:6835/149‑1 (MQ=255)

                                                                                                       gCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGc                                           <  8:275789/149‑1 (MQ=255)

                                                                                                        cTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGACTGGGCCAGCATGACCGGGCCGCAGGc                                                               <  5:224462/128‑1 (MQ=255)

                                                                                                        cTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGACTGGGCCAGCATGACCGGGCCGCAGGc                                                               >  6:224462/1‑128 (MQ=255)

                                                                                                         tCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCg                                                                                                            <  2:244012/82‑1 (MQ=255)

                                                                                                         tCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGc                                           >  3:257982/1‑147 (MQ=255)

                                                                                                         tCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGc                                           <  4:257982/147‑1 (MQ=255)

                                                                                                         tCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCg                                         >  7:264620/1‑149 (MQ=255)

                                                                                                           aaTCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCg                                         >  7:255518/1‑147 (MQ=255)

                                                                                                               gCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGAt                                    <  4:88252/148‑1 (MQ=255)

                                                                                                               gCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATg                                   <  2:357418/149‑1 (MQ=255)

                                                                                                               gCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATg                                   >  6:378508/1‑149 (MQ=255)

                                                                                                               gCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATg                                   <  1:294479/149‑1 (MQ=255)

                                                                                                               gCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATg                                   <  8:227838/149‑1 (MQ=255)

                                                                                                                 aaCAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATgg                                  >  7:167105/1‑148 (MQ=255)

                                                                                                                       gACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGc                           <  8:255518/149‑1 (MQ=255)

                                                                                                                        aCGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGc                           <  4:311091/148‑1 (MQ=255)

                                                                                                                          gAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCGTAGGGTGGGCAGGGTATGCCGCAGATGGCATCGGcctg                        >  1:107900/1‑147 (MQ=255)

                                                                                                                            aCAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCt                      >  8:159040/1‑149 (MQ=255)

                                                                                                                                     tCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCa                                                                  >  4:207142/1‑96 (MQ=255)

                                                                                                                                        gtgggtggGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATTCGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGAt                                    <  1:314176/123‑1 (MQ=255)

                                                                                                                                        gtgggtggGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCg                                                                    <  6:254270/91‑1 (MQ=255)

                                                                                                                                        gtgggtggGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCg                                                                    >  5:254270/1‑91 (MQ=255)

                                                                                                                                        gtgggtggGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGAt                                    >  2:314176/1‑123 (MQ=255)

                                                                                                                                        gtgggtggGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGc          <  7:219478/149‑1 (MQ=255)

                                                                                                                                        gtgggtggGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGc          >  7:64682/1‑149 (MQ=255)

                                                                                                                                           ggtgggCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGa       >  1:225229/1‑149 (MQ=255)

                                                                                                                                            gtgggCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGAt      <  8:167105/149‑1 (MQ=255)

                                                                                                                                            gtgggCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCGGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGTAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGAt      <  1:269437/149‑1 (MQ=255)

                                                                                                                                             ggggTGGTGACCTCACCGCCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGATg     >  8:70400/2‑149 (MQ=255)

                                                                                                                                             ggggTGGTGACCTCACCGCCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGATg     >  2:37735/2‑149 (MQ=255)

                                                                                                                                              gggTGGTGACCTCACCGCCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCa                         <  3:359519/128‑1 (MQ=255)

                                                                                                                                              gggTGGTGACCTCACCGCCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCa                         >  4:359519/1‑128 (MQ=255)

                                                                                                                                              gggTGGTGACCTCACCGCCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGATCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGATgg    >  1:237339/1‑149 (MQ=255)

                                                                                                                                              gggCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGATgg    >  6:186321/1‑149 (MQ=255)

                                                                                                                                                gCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGATggcg  <  7:159040/149‑1 (MQ=255)

ACCTGAGCGGACATTGCCAGCAACTCGGTATCGAACTGGACCGCGACACCCTCGACGGCATCCTTGAGGAGGCCGCACGTTTCACTGGCCAGATCATCGCACCGCTCAATCGCAACAGTGACGAACAGGGCTGTCAGTGGGTGGGCGGTGAGGTCACCACCCCGGACGGCATGCGGGACGCCTATCGGCAATACGTCGAACATGGCTGGGCCAGCATGACCGGGCCGCAGGCCTATGGTGGGCAGGGTCTGCCGCAGATGGCATCGGCCAGCTTCCATGAAATGCTGATGGCG  >  NC_002947/2329703‑2329995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`