Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,402,311 A→T 5.9% L19Q (CTG→CAG)  PP_2103 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,402,3110AT 5.9% 429.5 / 9.7 135L19Q (CTG→CAG) PP_2103hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (59/67);  new base T (3/5);  total (63/72)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 138 total aligned reads displayed.

TGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCACGCC  >  NC_002947/2402171‑2402458
                                                                                                                                             |                                                                                                                                                    
  ccccACAGTGGCGCATGCAGGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGCAAGGCGCTGCTGCGCCtgc                                                                                                                                              <  8:269504/148‑3 (MQ=255)
   cccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcg                                                                                                                                            >  7:134527/1‑148 (MQ=255)
   cccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcg                                                                                                                                            >  6:360535/1‑148 (MQ=255)
   cccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcg                                                                                                                                            >  7:200206/1‑148 (MQ=255)
    ccACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTgcg                                                                                                                                            >  6:265023/1‑147 (MQ=255)
     cacaGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGCAAGGCGCTGCTGCGCC‑TGCGCat                                                                                                                                         <  8:122813/149‑2 (MQ=255)
     cacaGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAg                                                                                                                                         >  3:33337/1‑149 (MQ=255)
        aGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTc                                                                                                                                      >  4:346724/1‑149 (MQ=255)
            gcgcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcgc                                                                                                                                  >  6:301195/1‑149 (MQ=255)
            gcgcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcgc                                                                                                                                  <  4:174865/149‑1 (MQ=255)
             cgcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTcgcgcg                                                                                                                                 >  7:131857/1‑149 (MQ=255)
              gcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCgg                                                                                                                                >  5:195115/1‑149 (MQ=255)
              gcATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCgg                                                                                                                                <  8:188827/149‑1 (MQ=255)
                    ttGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcg                                                                                                                          >  1:73624/1‑149 (MQ=255)
                    ttGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcg                                                                                                                          >  6:83758/1‑149 (MQ=255)
                    ttGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcg                                                                                                                          >  7:100601/1‑149 (MQ=255)
                    ttGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTCACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTAcg                                                                                                                          >  7:221576/1‑149 (MQ=255)
                        tACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCgtg                                                                                                                      <  8:114525/149‑1 (MQ=255)
                           ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGCAAGGCGCTGCTGCGCCtgc                                                                                                                                              >  2:16590/1‑121 (MQ=255)
                           ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGttt                                                                                                                   >  6:159570/1‑149 (MQ=255)
                           ccAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGttt                                                                                                                   >  5:109174/1‑149 (MQ=255)
                            cAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAGGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTg                                                                                                                  >  6:165073/1‑149 (MQ=255)
                            cAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTg                                                                                                                  >  7:253447/1‑149 (MQ=255)
                             aGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTgg                                                                                                                 >  5:149395/1‑149 (MQ=255)
                               gCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCa                                                                                                               <  3:346724/149‑1 (MQ=255)
                                   tCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCgg                                                                                                                                >  4:204883/1‑128 (MQ=255)
                                   tCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCgg                                                                                                                                <  3:204883/128‑1 (MQ=255)
                                    cATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCt                                                                                                          >  1:276672/1‑149 (MQ=255)
                                     aTGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCt                                                                                                          <  5:159570/148‑1 (MQ=255)
                                     aTGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCt                                                                                                          <  8:131857/148‑1 (MQ=255)
                                     aTGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTg                                                                                                         >  4:306563/1‑149 (MQ=255)
                                      tGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTgg                                                                                                        <  5:112898/149‑1 (MQ=255)
                                        tAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTgg                                                                                                        >  1:304080/1‑147 (MQ=255)
                                        tAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCa                                                                                                      <  5:234844/149‑1 (MQ=255)
                                         aGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAg                                                                                                     >  8:64696/1‑149 (MQ=255)
                                             aGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTc                                                                                                 <  6:195115/149‑1 (MQ=255)
                                                  cccATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATgg                                                                                             >  4:360371/1‑148 (MQ=255)
                                                  cccATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATgg                                                                                             <  3:64094/148‑1 (MQ=255)
                                                  cccATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATgg                                                                                             >  7:30787/1‑148 (MQ=255)
                                                  cccATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGc                                                                                            <  2:304080/149‑1 (MQ=255)
                                                    cATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCaa                                                                                          >  5:294091/1‑149 (MQ=255)
                                                        ccGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAgg                                                                                      >  3:364022/1‑149 (MQ=255)
                                                        ccGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAgg                                                                                      <  4:364022/149‑1 (MQ=255)
                                                         cgcgCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGgc                                                                                     >  6:142433/1‑149 (MQ=255)
                                                         cgcgCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGgc                                                                                     >  2:157973/1‑149 (MQ=255)
                                                           cggaCGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGgcgc                                                                                   <  1:315704/146‑1 (MQ=255)
                                                              aCGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑cc                                                                               <  3:295521/149‑1 (MQ=255)
                                                                 cggcgCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTg                                                                            >  4:198864/1‑149 (MQ=255)
                                                                   gcgcAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTc                                                                          <  8:200206/149‑1 (MQ=255)
                                                                    cgcAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTcc                                                                         <  7:267930/149‑1 (MQ=255)
                                                                      cAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCtt                                                                       <  5:142433/149‑1 (MQ=255)
                                                                       aGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTg                                                                      <  5:308953/149‑1 (MQ=255)
                                                                       aGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTg                                                                      <  5:301195/149‑1 (MQ=255)
                                                                                aGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑cc                                                                               >  2:294870/1‑131 (MQ=255)
                                                                                aGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTtcg                                                             >  7:235227/1‑149 (MQ=255)
                                                                                aGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTtcg                                                             >  5:10901/1‑149 (MQ=255)
                                                                                aGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTtcg                                                             >  4:140484/1‑149 (MQ=255)
                                                                                 gCAACTGATCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTtcgt                                                            >  2:293778/1‑149 (MQ=255)
                                                                                 gCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCt                                                                             <  4:344188/132‑1 (MQ=255)
                                                                                 gCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCt                                                                             >  3:344188/1‑132 (MQ=255)
                                                                                    aCTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTtcgtc                                                           >  5:43729/1‑147 (MQ=255)
                                                                                       gACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAggg                                                      >  8:225727/1‑149 (MQ=255)
                                                                                       gACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAggg                                                      >  7:91046/1‑149 (MQ=255)
                                                                                        atcGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGt                                                     <  1:293778/147‑1 (MQ=255)
                                                                                        aCCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAgg                                                       <  6:109174/147‑1 (MQ=255)
                                                                                        aCCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAgg                                                       <  3:140484/147‑1 (MQ=255)
                                                                                            gACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGgcg                                                 >  5:95515/1‑149 (MQ=255)
                                                                                              cTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGcc                                               <  3:34704/149‑1 (MQ=255)
                                                                                               tCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGcc                                               >  1:352408/1‑148 (MQ=255)
                                                                                                 aTGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCg                                              >  1:89731/1‑147 (MQ=255)
                                                                                                    aCGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAggg                                         <  6:294091/149‑1 (MQ=255)
                                                                                                     cGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGc                                        <  2:89731/149‑1 (MQ=255)
                                                                                                     cGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGc                                        <  4:336517/149‑1 (MQ=255)
                                                                                                       gATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGc                                                                                            >  3:166344/1‑96 (MQ=255)
                                                                                                       gATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGc                                                                                            <  4:166344/96‑1 (MQ=255)
                                                                                                       gATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCga                                      <  2:73624/149‑1 (MQ=255)
                                                                                                       gATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCga                                      <  8:235227/149‑1 (MQ=255)
                                                                                                       gATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCga                                      <  8:221576/149‑1 (MQ=255)
                                                                                                        aTTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCgat                                     <  3:306563/149‑1 (MQ=255)
                                                                                                           tCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCgatgat                                  >  1:214366/1‑149 (MQ=255)
                                                                                                            ccTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCgatgat                                  <  1:157973/148‑1 (MQ=255)
                                                                                                             cTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGttt                                                                                                                   <  6:133512/67‑1 (MQ=255)
                                                                                                              tGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtcg                               >  2:217274/1‑149 (MQ=255)
                                                                                                                gCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCTCCCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtc                                <  1:81382/147‑1 (MQ=255)
                                                                                                                gCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGc                                        >  7:289230/1‑138 (MQ=255)
                                                                                                                    tGCAATTCCATGCGC‑AGGCGCAGCCGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGATCTGGCAGGCACATGGCAACAGGCGCT‑CCCAGTCCTTCGCAAGTACGTAGAGGGTGGCGCCGAAGCGCGATGATTCGTCGaa                          >  4:327073/1‑148 (MQ=255)
                                                                                                                       aTTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACAcc                      <  4:143536/149‑1 (MQ=255)
                                                                                                                          tCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACAccgc                    <  8:30787/148‑1 (MQ=255)
                                                                                                                          gaactGCGCAAGGCGCTGCTGCGCC‑TGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACAccgcc                   >  2:34235/5‑149 (MQ=255)
                                                                                                                                cgc‑aggcgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTAGAACACCGCCGCCtt              >  7:277052/1‑148 (MQ=255)
                                                                                                                                 gc‑aggcgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCga                                      >  8:273011/1‑123 (MQ=255)
                                                                                                                                  caaggcgctgCTGCGCC‑TGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAg           <  1:34235/147‑1 (MQ=255)
                                                                                                                                  c‑aggcgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAg           >  3:10219/1‑149 (MQ=255)
                                                                                                                                  c‑aggcgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAg           <  7:795/149‑1 (MQ=255)
                                                                                                                                     ggcgctgCTGCTCC‑TGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAggtg        <  1:326334/149‑3 (MQ=255)
                                                                                                                                      gcgcagCAGCGCGTTGATCAGTTCGCGCTGGTTACGCGTGTTTGGCAGTTCTTTCAGGCTCATGGCAAGAGGCGCT‑CCCTGTCCTTGGCAAGTTCTTCGAGGGTGGCGCCGAAGGTCGATGATTCGTCGAACACCGCCGCCTTCAGgc         <  2:119624/148‑1 (MQ=255)
                                                                                                                                      gcgcagCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGGAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCgatgat                                  <  8:255540/123‑1 (MQ=255)
                                                                                                                                        gcagcaGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtc                                <  2:110014/123‑1 (MQ=255)
                                                                                                                                        gcagcaGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATtc                                >  1:110014/1‑123 (MQ=255)
                                                                                                                                            cagcGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCAcgcc  >  1:165717/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
TGCCCCACAGTGGCGCATGCTTGATACCCAGGCCTTCATGTAGCGAGCTGCCCATACCGCGCACGCGGCGCAGCGGTTCAAGCAACTGACCGGACTCATGACGGATTTCCTGGCGGTGCATTTCCATGCGC‑AGGCGCAGCAGCGCCTTGCGCAGTTCGCGCGGGTTACGCGTGTTTGGCAGTTCTGGCAGGCTCATGGCAACAGGCGCT‑CCCTGTCCTTGGCAAGTTCGTCGAGGGTGGCGCCGAAGGGCGATGATTCGTCGAACACCGCCGCCTTCAGGCGCACGCC  >  NC_002947/2402171‑2402458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑