Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,443,500 T→A 5.5% F6I (TTT→ATT) ‡ PP_2140 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,443,5000TA 5.5% 345.1 / 6.5 108F6I (TTT→ATT) ‡PP_2140hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (52/50);  new base A (3/3);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 108 total aligned reads displayed.

ATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACA  >  NC_002947/2443356‑2443645
                                                                                                                                                |                                                                                                                                                 
aTCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTg                                                                                                                                                <  8:202674/148‑1 (MQ=255)
aTCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGc                                                                                                                                               <  7:125112/149‑1 (MQ=255)
aTCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGc                                                                                                                                               >  5:29592/1‑149 (MQ=255)
   cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCgg                                                                                                                                             <  7:118887/148‑1 (MQ=255)
   cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGAAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGc                                                                                                                                            >  1:277269/1‑149 (MQ=255)
    gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGcc                                                                                                                                           <  4:82381/149‑1 (MQ=255)
    gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGcc                                                                                                                                           <  6:286170/149‑1 (MQ=255)
    gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGcc                                                                                                                                           >  5:286170/1‑149 (MQ=255)
        cAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCaa                                                                                                                                       >  3:288640/1‑149 (MQ=255)
        cAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCa                                                                                                                                        <  8:149083/148‑1 (MQ=255)
           ccGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                    >  8:44798/1‑149 (MQ=255)
             gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAtt                                                                                                                                  >  8:5761/1‑149 (MQ=255)
             gCAAGACCAAGGAGCAGTATGTGCAGGCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTGGGGCCGCAAAGCGGCCCCaaatcaca                                                                                                                                 <  8:277323/149‑7 (MQ=255)
                    cAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGc                                                                                                                                            >  6:212557/1‑132 (MQ=255)
                    cAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGc                                                                                                                                            <  5:212557/132‑1 (MQ=255)
                      aGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCAGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTatgatg                                                                                                                           >  8:65783/1‑147 (MQ=255)
                        gAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGa                                                                                                                       <  8:323601/149‑1 (MQ=255)
                         aGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGat                                                                                                                      <  6:29592/149‑1 (MQ=255)
                             ttATGTGCAGTCCGAGGTCGATGGGAAGCCTACCGGCTGGAAGGCATTCTACGACTGCAATGCCTGGAAGGTTGAATATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATcc                                                                                                                  <  6:342922/148‑1 (MQ=255)
                             gTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTTGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATcc                                                                                                                  >  1:131023/1‑149 (MQ=255)
                              tATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCg                                                                                                                 >  8:63557/1‑149 (MQ=255)
                                tgtgCAGTCCGATGTCGATGGCAAGCCGACCGGCTTGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGcc                                                                                                               <  7:147084/149‑1 (MQ=255)
                                tgtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGcc                                                                                                               >  4:272371/1‑149 (MQ=255)
                                   gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCa                                                                                                                         >  6:83204/1‑136 (MQ=255)
                                   gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCa                                                                                                                         <  5:83204/136‑1 (MQ=255)
                                   gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                            >  7:33392/1‑149 (MQ=255)
                                   gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                            >  7:355379/1‑149 (MQ=255)
                                     aGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGACATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                            >  3:62809/1‑147 (MQ=255)
                                       tCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                        >  7:346880/1‑149 (MQ=255)
                                       tCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                        >  5:106876/1‑149 (MQ=255)
                                       tCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                        <  6:4357/149‑1 (MQ=255)
                                         cGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGcc                                                                                                       <  8:355379/148‑1 (MQ=255)
                                           aGGTCGATGGCAAGCCGACCGGCTTGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTa                                                                                                    <  2:131023/149‑1 (MQ=255)
                                           aGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCtga                                                                                                    >  7:138423/1‑147 (MQ=255)
                                             gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTa                                                                                                                                >  6:346580/1‑119 (MQ=255)
                                             gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTa                                                                                                                                <  5:346580/119‑1 (MQ=255)
                                             gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                        >  6:111365/1‑143 (MQ=255)
                                             gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                        <  5:111365/143‑1 (MQ=255)
                                                gATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATgg                                                                                                 >  8:281948/1‑147 (MQ=255)
                                                gATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGa                                                                                               <  7:5761/149‑1 (MQ=255)
                                                    gCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAggg                                                                                            <  2:277269/148‑1 (MQ=255)
                                                    gCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAggg                                                                                            <  1:89062/148‑1 (MQ=255)
                                                    gCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGc                                                                                           <  8:346880/149‑1 (MQ=255)
                                                    gCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGAGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTAt                                                                                                   <  5:337520/141‑1 (MQ=255)
                                                    gCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGAGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTAt                                                                                                   >  6:337520/1‑141 (MQ=255)
                                                      aaGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCAc                                                                                         <  4:288640/149‑1 (MQ=255)
                                                      aaGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGATGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCAGCCTTGTAGCCTTATGGGAggg                                                                                            >  3:208888/1‑146 (MQ=255)
                                                        gCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTa                                                                                                          >  4:106803/1‑130 (MQ=255)
                                                        gCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTa                                                                                                          <  3:106803/130‑1 (MQ=255)
                                                               ggCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGc                                                                                <  8:19881/149‑1 (MQ=255)
                                                               ggCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGc                                                                                >  2:111358/1‑149 (MQ=255)
                                                                 cTGGAAGGCATTCTTCGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTATCCTTATGGGAGGGCACTGAAATGGccc                                                                              <  3:279354/149‑1 (MQ=255)
                                                                  tGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGcc                                                                               >  1:211758/1‑147 (MQ=255)
                                                                    gAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                            >  2:216755/1‑148 (MQ=255)
                                                                       ggCATTCTACGACTGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAAc                                                                        <  4:8278/149‑1 (MQ=255)
                                                                         cATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGa                                                                                               <  4:44204/124‑1 (MQ=255)
                                                                         cATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGa                                                                                               >  3:44204/1‑124 (MQ=255)
                                                                          aTTCTAAGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActc                                                                      >  6:87601/1‑148 (MQ=255)
                                                                            tCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTa                                                                    >  1:97987/1‑148 (MQ=255)
                                                                             cTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTaca                                                                  >  2:231607/1‑149 (MQ=255)
                                                                             cTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTaca                                                                  >  4:166979/1‑149 (MQ=255)
                                                                             cTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTac                                                                   <  3:333184/148‑1 (MQ=255)
                                                                               acgacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACAcc                                                                >  5:309263/1‑149 (MQ=255)
                                                                                cgacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActc                                                                      >  3:303742/1‑142 (MQ=255)
                                                                                cgacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActc                                                                      <  4:303742/142‑1 (MQ=255)
                                                                                 gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                        <  2:240770/123‑1 (MQ=255)
                                                                                 gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                        >  1:240770/1‑123 (MQ=255)
                                                                                 gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCg                                                              >  4:274526/1‑149 (MQ=255)
                                                                                 gacgGCAATGCCTGGAAGGGTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCg                                                              >  3:142927/1‑149 (MQ=255)
                                                                                   cgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTa                                                            <  7:63557/149‑1 (MQ=255)
                                                                                       aaTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCAAATAGGCTTATGGGAGGGCACTGAAATGGGCCAGGAACTCTACACCCGTtccaa                                                        >  1:304498/1‑149 (MQ=255)
                                                                                        aTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                       <  2:97987/149‑1 (MQ=255)
                                                                                           ccTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaacca                                                     <  7:281948/148‑1 (MQ=255)
                                                                                             tGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGaa                                                                         <  7:72314/126‑1 (MQ=255)
                                                                                               gAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                 <  1:231607/148‑1 (MQ=255)
                                                                                               gAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                 <  2:211758/148‑1 (MQ=255)
                                                                                                 aGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGATTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTg                                              <  3:272371/149‑1 (MQ=255)
                                                                                                    ttGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttc                                           >  3:161750/1‑149 (MQ=255)
                                                                                                          aTAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTAGGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGcc                                     >  8:261829/1‑149 (MQ=255)
                                                                                                            aaGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATTCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActc                                                                      >  5:302927/1‑114 (MQ=255)
                                                                                                            aaGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActc                                                                      <  6:302927/114‑1 (MQ=255)
                                                                                                             aGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCgg                                   <  3:274526/148‑1 (MQ=255)
                                                                                                              gcgcTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGcc                                 >  2:328197/1‑149 (MQ=255)
                                                                                                              gcgcTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGcc                                 <  4:161750/149‑1 (MQ=255)
                                                                                                                  tGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGc                             >  6:321868/1‑149 (MQ=255)
                                                                                                                       cATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                         >  1:64927/1‑148 (MQ=255)
                                                                                                                        aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                         >  2:40449/1‑147 (MQ=255)
                                                                                                                        aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGa                       <  5:87601/149‑1 (MQ=255)
                                                                                                                        aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGa                       <  3:166979/149‑1 (MQ=255)
                                                                                                                             tGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCa                  >  4:50728/1‑149 (MQ=255)
                                                                                                                              gcggggATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCGTCGCCGGCCTCGCCCTCGAGTc                    <  7:57784/141‑1 (MQ=255)
                                                                                                                                      ttGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                 >  1:22838/1‑109 (MQ=255)
                                                                                                                                      ttGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                 <  2:22838/109‑1 (MQ=255)
                                                                                                                                        ggggCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGc        <  1:40449/148‑1 (MQ=255)
                                                                                                                                         gggCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCt       >  3:184582/1‑148 (MQ=255)
                                                                                                                                           gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGaa    >  4:174752/1‑149 (MQ=255)
                                                                                                                                           gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGa     >  4:180370/1‑148 (MQ=255)
                                                                                                                                            ccGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAc   <  2:304498/149‑1 (MQ=255)
                                                                                                                                              gCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACa  >  4:360056/1‑148 (MQ=255)
                                                                                                                                              gCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACa  >  3:305438/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACA  >  NC_002947/2443356‑2443645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: