Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,474,162	A→G	11.9%	intergenic (‑67/+113)	PP_2167 ← / ← tal	PhoD‑like phosphatase/transaldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,474,162	0	A	G	11.9%	106.8 / 5.9	42	intergenic (‑67/+113)	PP_2167/tal	PhoD‑like phosphatase/transaldolase
Reads supporting (aligned to +/- strand):  ref base A (19/18);  new base G (3/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01

CGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCTTTTC  >  NC_002947/2474020‑2474310                                                                                                                                               |                                                                                                                                                     cggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGc                                                                                                                                                <  1:83581/149‑1 (MQ=255)       gacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGccc                                                                                                                                          <  5:256391/149‑1 (MQ=255)         cgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCcgc                                                                                                                                        <  8:105840/149‑1 (MQ=255)                                                   aGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGcaaa                                                                                              <  2:116710/149‑1 (MQ=255)                                                        cAACGGGTTAGGGGAGGTCATCGACAGCTCGGGGGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAAt                                                                                                                   <  5:221509/123‑1 (MQ=255)                                                              gTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGCCcgc                                                                                                                                        <  8:182023/96‑1 (MQ=14)                                                              gTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGCCcgc                                                                                                                                        >  7:182023/1‑96 (MQ=14)                                                                     gAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCGTACATCGATAAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAAATCAATAAACAAATAAAGCGTGGCAAAAAAAACGGGGGGaaaaa                                                                             >  4:243090/1‑147 (MQ=38)                                                                           aTCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGaa                                                                       <  3:243090/148‑1 (MQ=255)                                                                            tCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCg                                                                                                     <  7:157477/117‑1 (MQ=255)                                                                               aCAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCg                                                                                   <  1:133129/132‑1 (MQ=255)                                                                                             tATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGt                                                                                                    <  3:370821/101‑1 (MQ=255)                                                                                             tATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAgg                                                     >  7:182197/1‑148 (MQ=255)                                                                                                   ggcCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAacac                                              >  1:12237/1‑149 (MQ=255)                                                                                                       tGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAAc                                          >  2:55460/1‑149 (MQ=255)                                                                                                       tGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGCCGTGGCAAACAAAACGGGCGGAAAATACTGAAAACTTACCGCCCGTAAGGCAAACACCAAc                                          >  8:140541/1‑149 (MQ=39)                                                                                                        gTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACt                                         >  4:269015/1‑149 (MQ=255)                                                                                                         ttCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAAc                                          <  8:182197/147‑1 (MQ=255)                                                                                                         ttCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTg                                        >  4:331041/1‑149 (MQ=255)                                                                                                         ttCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGCAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAAc                                          <  8:43366/147‑1 (MQ=255)                                                                                                          tCGCGGGAAAGCCCGCCCTTACATCGATAAGTGTATGAGCGGGCTTGCCCGCGAACAGGCCAGAGAATTGAATACACAAATAAAGCCTGGCAAACAAAACGCGCCGAAAATACTTAACACTTACCCCCCGTAAGGAAAAAACGAAcag                                        >  2:95412/1‑146 (MQ=255)                                                                                                            gcgGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTcccgc                                                             >  4:223734/1‑125 (MQ=255)                                                                                                            gcgGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTcccgc                                                             <  3:223734/125‑1 (MQ=255)                                                                                                                gCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAAt                                                                                                                   <  4:319219/67‑1 (MQ=255)                                                                                                                gCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAAt                                                                                                                   >  3:319219/1‑67 (MQ=255)                                                                                                                 cAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGc                                <  2:12237/149‑1 (MQ=255)                                                                                                                    gcccgctcCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCa                                   >  3:47690/1‑143 (MQ=37)                                                                                                                    gcccgctcCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCa                                   <  4:47690/143‑1 (MQ=37)                                                                                                                    gcccgctcCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtt                             >  3:131378/1‑149 (MQ=37)                                                                                                                       cgcccTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtttt                           >  3:44678/1‑148 (MQ=255)                                                                                                                            ttACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTa                     >  1:241178/1‑149 (MQ=255)                                                                                                                                aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCa                         >  7:246680/1‑141 (MQ=255)                                                                                                                                aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCa                         <  8:246680/141‑1 (MQ=255)                                                                                                                                aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCaa                 >  7:31084/1‑149 (MQ=255)                                                                                                                                aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCa                  >  6:254451/1‑148 (MQ=255)                                                                                                                                    aTCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACtt              >  2:84993/1‑148 (MQ=255)                                                                                                                                     tCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTtct            <  3:331041/149‑1 (MQ=255)                                                                                                                                        gtgtAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTtctc           >  7:238358/1‑147 (MQ=255)                                                                                                                                        gtgtAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTtctc           >  3:57484/1‑147 (MQ=255)                                                                                                                                         tgtAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAg        <  5:254451/149‑1 (MQ=255)                                                                                                                                             ggAGCGGGCTTGCCCGCGAACAGGCCCGATAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCttt    <  7:140541/149‑1 (MQ=255)                                                                                                                                              gAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCtttt   >  2:14127/1‑149 (MQ=255)                                                                                                                                               aGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACGCAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGACCACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCTTTTc  >  4:240383/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                     CGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCTTTTC  >  NC_002947/2474020‑2474310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑