Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,536,624 G→A 5.9% I805I (ATC→ATT)  mrpAB ← K(+)/H(+) antiporter subunit A/B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,536,6240GA 5.9% 210.2 / 2.2 68I805I (ATC→ATT) mrpABK(+)/H(+) antiporter subunit A/B
Reads supporting (aligned to +/- strand):  ref base G (30/34);  new base A (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

GACAAACCCGCCACCGGGCTGGTTGTGCCCGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGG  >  NC_002947/2536483‑2536762
                                                                                                                                             |                                                                                                                                           
gACAAACCCGCCACCGGGCTGGTTGTGCCCGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGt                                                                                                                                      >  5:122418/1‑149 (MQ=255)
   aaaCCCGCCACCGGGCTGGTTGTGCCCGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCgg                                                                                                                                   >  1:1138/1‑149 (MQ=255)
          cctccGGGCTGGGTGTGCCCGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCAc                                                                                                                            <  3:312017/146‑1 (MQ=255)
                ggCTGGTTGTGCCCGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTgg                                                                                                                      <  6:122418/149‑1 (MQ=255)
                      ttGTGCCCGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCaa                                                                                                                <  4:273280/149‑1 (MQ=255)
                         tgCCCGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTg                                                                                                             <  6:219724/149‑1 (MQ=255)
                           cccGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTAATCAGGTCGGTGACCACGTCTGGCGCCAACTGGc                                                                                                           >  6:132860/1‑149 (MQ=255)
                            ccGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCg                                                                                                                                    <  4:345319/123‑1 (MQ=255)
                            ccGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCg                                                                                                                                    >  3:345319/1‑123 (MQ=255)
                            ccGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCg                                                                                                          >  4:203652/1‑149 (MQ=255)
                                     aaCAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTgcgc                                                                                                 >  1:22370/1‑149 (MQ=255)
                                     aaCAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTgcgc                                                                                                 <  5:108907/149‑1 (MQ=255)
                                          gTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGc                                                                                             >  6:12720/1‑148 (MQ=255)
                                            aCATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAAc                                                                                          >  4:120638/1‑149 (MQ=255)
                                             cATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACt                                                                                         <  2:1138/149‑1 (MQ=255)
                                                  acacCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATg                                                                                    >  7:355982/1‑149 (MQ=255)
                                                      cAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGctct                                                                                >  6:323263/1‑149 (MQ=255)
                                                      cAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGct                                                                                  <  1:134408/147‑1 (MQ=255)
                                                      cAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGct                                                                                  >  2:134408/1‑147 (MQ=255)
                                                        gCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGctctc‑t                                                                             <  4:11234/149‑1 (MQ=255)
                                                           aGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTAATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTc                                                                          <  5:132860/149‑1 (MQ=255)
                                                             gCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGctc                                                                                 >  5:324101/1‑141 (MQ=255)
                                                             gCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGctc                                                                                 <  6:324101/141‑1 (MQ=255)
                                                               gATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCATTTTGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAg                                                                      <  1:119104/149‑1 (MQ=255)
                                                               gATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAg                                                                      >  3:94250/1‑149 (MQ=255)
                                                                   ggCAGCAGCAGGCGCACCAATGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCg                                                                  <  7:282717/149‑1 (MQ=255)
                                                                           cagGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGcc                                                          >  5:101911/1‑149 (MQ=255)
                                                                              gcgcACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCg                                                         >  8:293745/1‑147 (MQ=255)
                                                                              gcgcACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCg                                                         >  1:217081/1‑147 (MQ=255)
                                                                                gcACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACGACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGc                                                     <  1:166346/149‑1 (MQ=255)
                                                                                  aCCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAg                                                   >  4:2973/1‑149 (MQ=255)
                                                                                  aCCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATCCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAg                                                   >  6:32977/1‑149 (MQ=255)
                                                                                   ccAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAgg                                                  <  3:120638/149‑1 (MQ=255)
                                                                                   ccAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAgg                                                  <  6:120407/149‑1 (MQ=255)
                                                                                    cAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAggg                                                 >  5:194629/1‑149 (MQ=255)
                                                                                     aaCGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAgg                                                  <  5:117628/147‑1 (MQ=255)
                                                                                     aaCGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAgg                                                  >  6:117628/1‑147 (MQ=255)
                                                                                     aaCGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGc                                                >  2:78731/1‑149 (MQ=255)
                                                                                     aaCGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGc                                                <  3:203652/149‑1 (MQ=255)
                                                                                       cGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAggtggg                                              >  4:66703/1‑145 (MQ=255)
                                                                                        gCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGaa                                             <  6:101911/149‑1 (MQ=255)
                                                                                            cAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACg                                         <  5:323263/149‑1 (MQ=255)
                                                                                             aGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACgg                                        <  5:12720/149‑1 (MQ=255)
                                                                                               gCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATg                                                                                    >  3:298882/1‑104 (MQ=255)
                                                                                               gCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATg                                                                                    <  4:298882/104‑1 (MQ=255)
                                                                                               gCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGt                                       >  4:32771/1‑148 (MQ=255)
                                                                                                 aCCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCaa                                    >  2:86748/1‑149 (MQ=255)
                                                                                                 aCCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCaa                                    <  4:226665/149‑1 (MQ=255)
                                                                                                 aCCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCaa                                    <  2:270645/149‑1 (MQ=255)
                                                                                                     tcatGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGcc                                <  7:293745/149‑1 (MQ=255)
                                                                                                       atGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCcac                              <  1:78731/149‑1 (MQ=255)
                                                                                                         gAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAg                            >  8:99706/1‑149 (MQ=255)
                                                                                                            gCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGcca                         >  7:347316/1‑149 (MQ=255)
                                                                                                            gCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGcca                         <  2:22370/149‑1 (MQ=255)
                                                                                                             cccAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGccac                        >  3:123733/1‑149 (MQ=255)
                                                                                                             cccAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGccac                        <  8:355982/149‑1 (MQ=255)
                                                                                                               cAGCGCGGTATCAGTTGCATGCCGCGGGTTGATCACGTCGGTGACCACGTCTTGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACg                                         <  1:242981/131‑1 (MQ=255)
                                                                                                               cAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACg                                         >  2:242981/1‑130 (MQ=255)
                                                                                                               cAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGc                                 <  5:244763/138‑1 (MQ=255)
                                                                                                               cAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGc                                 >  6:244763/1‑138 (MQ=255)
                                                                                                                 gcgcGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAAt                    <  6:294648/149‑1 (MQ=255)
                                                                                                                    cgGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACg                 <  3:32771/149‑1 (MQ=255)
                                                                                                                    cgGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACg                 <  3:2973/149‑1 (MQ=255)
                                                                                                                            gTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTc         >  4:345581/1‑149 (MQ=255)
                                                                                                                                 aTGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGa    <  6:194629/149‑1 (MQ=255)
                                                                                                                                    ccGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAgg  <  3:345581/148‑1 (MQ=255)
                                                                                                                                         ggTTAATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAgg  <  6:18587/143‑1 (MQ=255)
                                                                                                                                         ggTTAATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAgg  >  5:18587/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                           
GACAAACCCGCCACCGGGCTGGTTGTGCCCGCGCATGAACAGGTACATCGACACCAGCAAGGCGATCGGCAGCAGCAGGCGCACCAACGCTGCAGGCACCATCATGAAGCCCAGCGCGGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTC‑TTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGG  >  NC_002947/2536483‑2536762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑