| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 200,340 | C→A | 8.0% | T1949N (ACC→AAC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 200,340 | 0 | C | A | 8.0% | 144.0 / 3.3 | 50 | T1949N (ACC→AAC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (22/24); new base A (2/2); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
GCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCAC > NC_002947/200192‑200481 | gCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGAc > 4:9654/1‑149 (MQ=255) gCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCGCTTCGGTGACCGaa < 3:48106/149‑1 (MQ=255) ccAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAg < 2:244702/149‑1 (MQ=255) cAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAgg > 8:80703/1‑149 (MQ=255) cAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAgg > 5:37379/1‑149 (MQ=255) aGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGc > 5:353015/1‑149 (MQ=255) gATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCg < 5:104883/149‑1 (MQ=255) gATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCg < 7:80703/149‑1 (MQ=255) tCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGt < 6:37379/149‑1 (MQ=255) ggCATGAACTCGGCAGGAGCCGCCACTGGTGCTGCGTTCGAGTACCTTGAGCTGGGCGGGTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGTAGGCGGTGAGATCa < 8:241000/148‑1 (MQ=14) gCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAcc < 1:315788/149‑1 (MQ=255) cAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTaca > 4:192941/1‑149 (MQ=255) aaCTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTacac < 6:353015/149‑1 (MQ=255) aCTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACAcc < 3:9654/149‑1 (MQ=255) tCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTga > 6:148573/1‑134 (MQ=255) tCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTga < 5:148573/134‑1 (MQ=255) ggCACTACACGCCACATGTGCTGCGTGACTCAACCATTAGCAGGGCGGGTCCGCTTCGGTTCAACTCACCGTCACCCTCGCCGAATGGGGATGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCa < 1:287910/148‑1 (MQ=2) gTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCt < 2:185986/136‑1 (MQ=40) gTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCt > 1:185986/1‑136 (MQ=40) tAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCt > 7:316871/1‑148 (MQ=38) gACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑GGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGAc < 5:103945/149‑1 (MQ=37) gCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACcaa < 3:192941/149‑1 (MQ=37) gTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGAACGAAGGCGGTga > 3:280941/1‑115 (MQ=255) gTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGAACGAAGGCGGTga < 4:280941/115‑1 (MQ=255) gctgcGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAT‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACgg > 2:186874/1‑149 (MQ=37) gagaACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTga < 4:298356/104‑1 (MQ=35) gagaACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTga > 3:298356/1‑104 (MQ=35) tGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCg < 2:58525/139‑1 (MQ=34) tGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCg > 1:58525/1‑139 (MQ=34) aGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCAc > 2:26060/1‑149 (MQ=37) gCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGAACGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACt > 7:243645/1‑149 (MQ=37) cGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAg > 5:41909/1‑149 (MQ=32) ggTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGAACGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGc < 8:243645/149‑1 (MQ=17) aaGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGa < 5:375648/147‑1 (MQ=32) aaGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGa > 6:375648/1‑147 (MQ=32) gTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGa < 4:91475/128‑1 (MQ=32) gTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGa > 3:91475/1‑128 (MQ=32) gACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGcac < 2:297761/149‑1 (MQ=32) ccTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcg > 6:131140/1‑149 (MQ=33) g‑tggCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGc > 3:284383/1‑149 (MQ=34) ccAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAAc < 1:186874/149‑1 (MQ=34) cAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACa < 8:316871/149‑1 (MQ=34) aGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAg > 4:188941/1‑148 (MQ=34) gACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGcacca > 6:53883/1‑149 (MQ=34) aCCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTc > 8:175337/1‑149 (MQ=32) aCCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGAACTTCAAGCTGACCGACGGCACCACcgtcgtcgt < 6:113600/125‑1 (MQ=17) aCCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGAACTTCAAGCTGACCGACGGCACCACcgtcgtcgt > 5:113600/1‑125 (MQ=17) ccccTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCg < 4:284383/149‑1 (MQ=32) caccTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCg > 3:102709/3‑149 (MQ=17) ccTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGc > 3:31008/1‑149 (MQ=32) cGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCAc < 7:175337/148‑1 (MQ=32) | GCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAA‑GTGG‑TGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCAC > NC_002947/200192‑200481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |