Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,571,110 T→C 11.3% intergenic (‑28/+16) PP_2254 ← / ← dctA‑III GntR family transcriptional regulator/C4‑dicarboxylate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,571,1100TC11.3% 112.0 / 6.2 44intergenic (‑28/+16)PP_2254/dctA‑IIIGntR family transcriptional regulator/C4‑dicarboxylate transport protein
Reads supporting (aligned to +/- strand):  ref base T (20/19);  new base C (2/3);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CAGTTGTTCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCACGGTGGCCAC  >  NC_002947/2570963‑2571257
                                                                                                                                                   |                                                                                                                                                   
cAGTTGTTCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATg                                                                                                                                                    <  3:252329/149‑1 (MQ=255)
        cGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCATGACGCCGGccct                                                                                                                                            >  7:325833/1‑147 (MQ=255)
         ggCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCATGACGCCGGccctt                                                                                                                                           <  8:325833/149‑4 (MQ=255)
         ggCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGATGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACg                                                                                                                                           >  4:272608/1‑149 (MQ=255)
           ccAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGtt                                                                                                                                         <  5:167467/149‑1 (MQ=255)
             aGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCATGACGCCGGcccttccag                                                                                                                                       <  2:108087/149‑8 (MQ=255)
                                       gCCGGGGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTg                                                                                                             <  2:119491/149‑1 (MQ=255)
                                         cGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCATGACGCCg                                                                                                                                                 <  6:2320/111‑1 (MQ=255)
                                         cGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCATGACGCCg                                                                                                                                                 >  5:2320/1‑111 (MQ=255)
                                              cgcCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGttgct                                                                                                      <  7:83277/149‑1 (MQ=255)
                                                 cATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGACGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGttgcttgc                                                                                                   >  7:355953/1‑149 (MQ=255)
                                                 cATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGttgcttgc                                                                                                   <  8:355953/149‑1 (MQ=255)
                                                       ccGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTgcgtggcg                                                                                             >  8:156979/1‑149 (MQ=255)
                                                       ccGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTgcgt                                                                                                 >  6:346691/1‑145 (MQ=255)
                                                       ccGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTgcgt                                                                                                 <  5:346691/145‑1 (MQ=255)
                                                             gCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTggggg                                                                                       >  2:353344/1‑149 (MQ=255)
                                                             gCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTgggg                                                                                        <  1:19449/148‑1 (MQ=255)
                                                             gCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTgggg                                                                                        >  2:19449/1‑148 (MQ=255)
                                                              ccAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTggggg                                                                                       >  3:305121/1‑148 (MQ=255)
                                                                 gggCGTGGCGGAGTTTTTCCCCCACTGAGTTGACGCCGGTTGATGCGCTGCTCTCGGTTCGCTCCTTGTAGGGCCGGCGTCATGCGGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCaaa                                                                                   <  2:100132/149‑1 (MQ=255)
                                                                      tGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGTAAAGCCAg                                                                              >  5:368636/1‑149 (MQ=255)
                                                                       ggCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAgg                                                                             <  1:353344/149‑1 (MQ=255)
                                                                                    caccaaTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAg                                                                 >  7:237550/1‑148 (MQ=255)
                                                                                       cacTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCt                                                                                                              >  8:192924/1‑100 (MQ=255)
                                                                                         cTGAGTTGACGACGTTTGATGAGCTGATCACGGTGCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTTCGTGGCGTGTGGGCAAAGCCAGGCTTGCCGTCGAGCACGtt                                                           <  2:108336/149‑1 (MQ=255)
                                                                                             gTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTgc                                                       >  8:74927/1‑149 (MQ=255)
                                                                                              ttGACTAGGGTTGTTGAGGTGTTCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTgcg                                                      <  4:305121/149‑1 (MQ=255)
                                                                                                          gatgaGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAgg                                           <  6:368636/148‑1 (MQ=255)
                                                                                                           atgaGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGt                                          >  6:39556/1‑148 (MQ=255)
                                                                                                                cTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATg                                     >  4:226024/1‑148 (MQ=255)
                                                                                                                   aTCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTcc                                  <  8:237550/148‑1 (MQ=255)
                                                                                                                    tCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCtt                                <  3:150290/149‑1 (MQ=255)
                                                                                                                     cACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTtc                               >  5:170941/1‑149 (MQ=255)
                                                                                                                      aCGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTtct                              <  3:272608/149‑1 (MQ=255)
                                                                                                                                ccTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACggg                     >  3:329833/1‑148 (MQ=255)
                                                                                                                                  tGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCaa                  >  5:252194/1‑149 (MQ=255)
                                                                                                                                    gAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATg                >  3:238238/1‑149 (MQ=255)
                                                                                                                                    gAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATg                >  6:2231/1‑149 (MQ=255)
                                                                                                                                        ggCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCa            >  3:215801/1‑149 (MQ=255)
                                                                                                                                         gCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCAc           <  6:252194/149‑1 (MQ=255)
                                                                                                                                             gCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCACGGTg       >  6:258250/1‑149 (MQ=255)
                                                                                                                                                  aTGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCACGGTGGCCa   <  4:329833/148‑1 (MQ=255)
                                                                                                                                                  aTGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCACGGTGGCCa   <  5:39556/148‑1 (MQ=255)
                                                                                                                                                  aTGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCACGGTGGCCAc  <  7:74927/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGTTGTTCGGCCAGTTCGCGCTGGCCTGGCAGCATGTCGCCGGTGCGCCATTGGCCGCGGGCCAGGGCCTGGCGGAGTTTTTCCACCACTGAGTTGACGACGGTTGATGAGCTGATCACGGTTCGCTCCTGGAAGGGCCGGCGTCATGCCGGCCACGTTCGCTCAGAATTCCTGTTGATGGGCGCTGGGTTGCTTGCGTGGCGTGGGGGCAAAGCCAGGCTTGCCGTCGAGCACGTTCTGCGCACGTTCCAGGTCAATGTCCTTCTCCCAACGGGCAATGGCCACGGTGGCCAC  >  NC_002947/2570963‑2571257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑