Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,627,695	C→T	6.6%	A260A (GCC→GCT) ‡	tig →	trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,627,695	0	C	T	6.6%	228.4 / 4.1	75	A260A (GCC→GCT) ‡	tig	trigger factor
Reads supporting (aligned to +/- strand):  ref base C (36/34);  new base T (3/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

CCAAGGCTGGCGAAGAGCGCGTGGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAATCGAAGTGC  >  NC_002947/2627547‑2627840                                                                                                                                                      |                                                                                                                                                    ccAAGGCTGGCGAAGAGCGCGTGGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGcc                                                                                                                                                     >  4:170588/1‑149 (MQ=255)          gCGAAGAGCGCGTTGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCCCCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑gg                                                                                                                                           <  1:332179/148‑1 (MQ=255)          gCGAAGAGCGCGTGGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGGGCGAA‑GAACTCTTCATTCAGT‑Tctg                                                                                                                                            <  3:337561/148‑3 (MQ=255)          gCGAAGAGCGCGTGGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑g                                                                                                                                            <  7:40549/147‑1 (MQ=255)               tagCGCGTGGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGGTGAGTTCACCATCACCCTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCaa                                                                                                                                     <  8:222804/148‑1 (MQ=255)                     gTGGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATc                                                                                                                               >  4:356545/1‑149 (MQ=255)                     gTGGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATc                                                                                                                               <  2:34327/149‑1 (MQ=255)                        gTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGAc                                                                                                                            <  4:132765/149‑1 (MQ=255)                           aaCGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGGACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGCATCGACGCt                                                                                                                         >  4:260772/1‑149 (MQ=255)                             cGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTgg                                                                                                                       <  1:19724/149‑1 (MQ=255)                             cGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTgg                                                                                                                       <  7:279507/149‑1 (MQ=255)                               tGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGaa                                                                                                                     <  3:170588/149‑1 (MQ=255)                               tGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGaa                                                                                                                     <  3:356545/149‑1 (MQ=255)                                gACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAg                                                                                                                    >  4:234752/1‑149 (MQ=255)                                            ggACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGTATCGACGCTGGAAGGCTTCCGCGCCg                                                                                                        <  2:215032/149‑1 (MQ=255)                                             gACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCg                                                                                                        >  4:181349/1‑148 (MQ=255)                                              aCTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGTA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCg                                                                                                        >  8:194887/1‑147 (MQ=255)                                               cTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGAATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAg                                                                                                     >  5:264030/1‑149 (MQ=255)                                                tACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAg                                                                                                                    <  5:228644/133‑1 (MQ=255)                                                tACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAg                                                                                                                    >  6:228644/1‑133 (MQ=255)                                                tACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGt                                                                                                    >  6:348642/1‑149 (MQ=255)                                                tACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGt                                                                                                    >  1:96319/1‑149 (MQ=255)                                                  ccAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTc                                                                                                  >  1:46739/1‑149 (MQ=255)                                                             acctggCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATg                                                                                       <  2:96319/149‑1 (MQ=255)                                                               ctggctggCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTg                                                                                                                        <  5:80481/114‑1 (MQ=255)                                                               ctggctggCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTg                                                                                                                        >  6:80481/1‑114 (MQ=255)                                                                  gctggcAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCg                                                                                  >  6:248043/1‑149 (MQ=255)                                                                  gctggcAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCg                                                                                  >  6:242556/1‑149 (MQ=255)                                                                      gcAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGATCATGGAGCGTGa                                                                               <  4:339293/148‑1 (MQ=255)                                                                      gcAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAg                                                                              <  3:164024/149‑1 (MQ=255)                                                                          aGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGc                                                                          <  3:234752/149‑1 (MQ=255)                                                                                  aGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAgg                                                                    <  5:197892/147‑1 (MQ=255)                                                                                    ttCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGa                                                                 >  3:327335/1‑148 (MQ=255)                                                                                    ttCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGAt                                                                <  5:242556/149‑1 (MQ=255)                                                                                    ttCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGAt                                                                <  5:248043/149‑1 (MQ=255)                                                                                      cACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATc                                                               <  5:309476/148‑1 (MQ=255)                                                                                         cATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAAGGAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATc                                                               >  1:273232/1‑146 (MQ=255)                                                                                         cATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGa                                                           <  5:95022/149‑1 (MQ=255)                                                                                         cATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGa                                                           <  5:348642/149‑1 (MQ=255)                                                                                         cATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGa                                                           >  6:66659/1‑149 (MQ=255)                                                                                         cATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGa                                                           >  2:310951/1‑149 (MQ=255)                                                                                              ccGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAg                                                      >  5:188164/1‑149 (MQ=255)                                                                                                 tCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAg                                                                     >  3:253038/1‑131 (MQ=255)                                                                                                 tCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAg                                                                     <  4:253038/131‑1 (MQ=255)                                                                                                   aaCAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGTAAC‑GGAATAAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTc                                                   >  1:102123/1‑148 (MQ=255)                                                                                                    aCAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTc                                                   >  3:87666/1‑146 (MQ=255)                                                                                                    aCAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTc                                                   >  3:68800/1‑146 (MQ=255)                                                                                                        cGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAAcc                                            <  6:188164/149‑1 (MQ=255)                                                                                                          tttCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAg                                          <  3:181349/149‑1 (MQ=255)                                                                                                              ggCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTc                                       <  2:273232/148‑1 (MQ=255)                                                                                                                cACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAg                                                      <  5:128228/131‑1 (MQ=255)                                                                                                                cACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAg                                                      >  6:128228/1‑131 (MQ=255)                                                                                                                      tACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAg                                                <  1:124669/131‑1 (MQ=255)                                                                                                                      tACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAg                                                >  2:124669/1‑131 (MQ=255)                                                                                                                          tgccgAACTGGGCGAA‑GAACTCTTCGTTCAGT‑TCTGGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAACAACCAGGACATGGACGGTctg                           >  7:48398/5‑149 (MQ=37)                                                                                                                           ccAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCTCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTctgct                         <  7:79261/149‑1 (MQ=255)                                                                                                                           ccAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTctgct                         >  1:365138/1‑149 (MQ=255)                                                                                                                           ccAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTctgct                         >  3:47849/1‑149 (MQ=255)                                                                                                                            cAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTctgctg                        >  7:349284/1‑149 (MQ=255)                                                                                                                            cAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTctgctg                        >  4:12722/1‑149 (MQ=255)                                                                                                                            cAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAACGTCAAGAACCAGGTCATGTACGGTctgctg                        >  4:292686/1‑149 (MQ=255)                                                                                                                                aCTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGgcc                     <  4:270511/148‑1 (MQ=255)                                                                                                                                 cTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGgccgc                   <  4:87666/149‑1 (MQ=255)                                                                                                                                  tgggcGAA‑GAACTCTTCGTTCAGT‑TCTGGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGgccgc                   >  8:46543/5‑149 (MQ=255)                                                                                                                                    aaCGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTc                                       <  6:358519/126‑1 (MQ=255)                                                                                                                                    aaCGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTc                                       >  5:358519/1‑126 (MQ=255)                                                                                                                                       gAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAAcc              >  2:350196/1‑148 (MQ=255)                                                                                                                                         a‑gagTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCaa           >  1:167866/1‑149 (MQ=255)                                                                                                                                           gagTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAAt          <  3:260772/149‑1 (MQ=255)                                                                                                                                             actcttcGTTCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAgg                                         >  3:117718/3‑116 (MQ=255)                                                                                                                                             actcttcGTTCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAgg                                         <  4:117718/114‑1 (MQ=255)                                                                                                                                              ttcttcGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAATCg        >  1:282238/1‑148 (MQ=255)                                                                                                                                                 ttcGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAATCGAAGt    >  5:179440/1‑149 (MQ=255)                                                                                                                                                 ttcGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAATCGAAGt    >  7:323076/1‑149 (MQ=255)                                                                                                                                                    gCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGCCGGTCTGCTGGCCGCCAACCCAATCGAAGTGc  >  6:36442/1‑148 (MQ=255)                                                                                                                                                    gCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAATCGAAGTGc  <  4:47849/148‑1 (MQ=255)                                                                                                                                                     cccAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTctgct                         <  7:151969/124‑1 (MQ=255)                                                                                                                                                     cccAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTctgct                         >  8:151969/1‑124 (MQ=255)                                                                                                                                                      ccAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAATCGaa      >  6:308592/1‑142 (MQ=255)                                                                                                                                                      ccAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAATCGaa      <  5:308592/142‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CCAAGGCTGGCGAAGAGCGCGTGGTCAACGTGACCTTCCCAGAGGACTACCAGAACCTGGACCTGGCTGGCAAAGCCGCTGAGTTCACCATCACCGTCAACAGCGTTTCGGCACCTGTACTGCCAGAACTGAACGAA‑GAGTTCTTCGCCCAGT‑TC‑GGCATCAAGGAATCGACGCTGGAAGGCTTCCGCGCCGAAGTTCGCAAGAACATGGAGCGTGAGCTGCGTCAGGCGATCAAGACCAAGGTCAAGAACCAGGTCATGGACGGTCTGCTGGCCGCCAACCCAATCGAAGTGC  >  NC_002947/2627547‑2627840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑