Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,628,386 A→C 6.2% A16A (GCA→GCC)  clpP → ATP‑dependent Clp protease proteolytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,628,3860AC 6.2% 243.4 / 6.5 80A16A (GCA→GCC) clpPATP‑dependent Clp protease proteolytic subunit
Reads supporting (aligned to +/- strand):  ref base A (39/36);  new base C (2/3);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

CTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAACTGC  >  NC_002947/2628240‑2628531
                                                                                                                                                  |                                                                                                                                                 
ctgAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCa                                                                                                                                                   <  2:111476/147‑1 (MQ=255)
 tgAGTAACGGTCTTCTCTCGTAGGAAAACTCCACAAGCCAGCATTCGAGCTGGCTTGTGCGTATTAAAGCCATGACTATTTGTGAGTGAGAGCAGGACATGTCCCGCAATTCTTATTTTCAGCAGAGCTCTGAAATCCAGTCCGCAggc                                                                                                                                                >  3:356733/1‑149 (MQ=255)
     tAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGcc                                                                                                                                            <  3:297972/149‑1 (MQ=255)
          gTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCCTGCGGCCTggat                                                                                                                                       <  6:371000/149‑3 (MQ=255)
               ctctcGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGa                                                                                                                                   <  6:13273/148‑1 (MQ=255)
                  tcGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATg                                                                                                                                 <  1:177676/147‑1 (MQ=255)
                    gTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATgg                                                                                                                                <  8:194022/146‑1 (MQ=255)
                    gTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTa                                                                                                                             >  8:244282/1‑149 (MQ=255)
                      aGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATc                                                                                                                           >  6:126832/1‑149 (MQ=255)
                       ggAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCCTGCGGCCTggatgtca                                                                                                                                   >  1:48163/1‑134 (MQ=255)
                       ggAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCCTGCGGCCTggatgtca                                                                                                                                   <  2:48163/140‑7 (MQ=255)
                              tCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTc                                                                                                                   >  4:181652/1‑149 (MQ=255)
                                cacaAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGAt                                                                                                                                  <  6:297157/132‑1 (MQ=255)
                                cacaAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGAt                                                                                                                                  >  5:297157/1‑132 (MQ=255)
                                        agccTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGc                                                                                                         <  8:81114/149‑1 (MQ=255)
                                           cTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGaa                                                                                                      >  7:189813/1‑149 (MQ=255)
                                            ttCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATgg                                                                                                                                <  6:141242/122‑1 (MQ=255)
                                            ttCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAAc                                                                                                     >  7:43954/1‑149 (MQ=255)
                                            ttCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATACAGGCCGCAGGCGGCCTGGTCCCGATgg                                                                                                                                >  5:141242/1‑122 (MQ=255)
                                             tCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGtt                                                                                                                              <  2:317442/123‑1 (MQ=255)
                                             tCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGtt                                                                                                                              >  1:317442/1‑123 (MQ=255)
                                                     gCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTAc                                                                                             <  1:286722/148‑1 (MQ=255)
                                                      cTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGa                                                                                           <  3:167996/149‑1 (MQ=255)
                                                       ttGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGa                                                                                           >  2:164463/1‑148 (MQ=255)
                                                          tgCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGAc                                                                                          >  3:206534/1‑146 (MQ=255)
                                                          tgCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGAc                                                                                          <  4:206534/146‑1 (MQ=255)
                                                             gTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTAc                                                                                    >  6:2764/1‑149 (MQ=255)
                                                                ttCAAGCGATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTc                                                                                  <  5:114276/148‑1 (MQ=255)
                                                                ttCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTc                                                                                  <  4:189362/148‑1 (MQ=255)
                                                                 tCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTcgc                                                                                >  3:22138/1‑149 (MQ=255)
                                                                  cAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTcgcg                                                                               >  5:324278/1‑149 (MQ=255)
                                                                   aaGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCTATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCgcgc                                                                              >  4:70887/1‑149 (MQ=255)
                                                                       cATGACTATTTGGGAGTGAGGGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGt                                                                          <  3:178746/149‑1 (MQ=255)
                                                                       cATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCTATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGt                                                                          <  3:70887/149‑1 (MQ=255)
                                                                        aTGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGtt                                                                         <  5:329581/149‑1 (MQ=255)
                                                                        aTGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGtt                                                                         >  8:31887/1‑149 (MQ=255)
                                                                          gACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTg                                                                        >  5:312941/1‑148 (MQ=255)
                                                                            cTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGaa                                                                                                      <  4:115616/116‑1 (MQ=255)
                                                                            cTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGaa                                                                                                      >  3:115616/1‑116 (MQ=255)
                                                                             tATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTg                                                                           >  4:368576/1‑142 (MQ=255)
                                                                             tATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTg                                                                           <  3:368576/142‑1 (MQ=255)
                                                                             tATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAg                                                                     >  5:35880/1‑148 (MQ=255)
                                                                               tttGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAg                                                                  >  6:182202/1‑149 (MQ=255)
                                                                               tttGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAg                                                                  >  5:42785/1‑149 (MQ=255)
                                                                                   ggAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCgtg                                                              <  7:31887/149‑1 (MQ=255)
                                                                                   ggAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCgt                                                               <  7:260398/148‑1 (MQ=255)
                                                                                   ggAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCgt                                                               >  8:260398/1‑148 (MQ=255)
                                                                                         aGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATc                                                         <  3:181652/148‑1 (MQ=255)
                                                                                         aGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCt                                                        >  2:364615/1‑149 (MQ=255)
                                                                                         aGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCt                                                        >  4:62848/1‑149 (MQ=255)
                                                                                                  cATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTg                                               <  6:324278/149‑1 (MQ=255)
                                                                                                     gTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGcc                                            >  4:60924/1‑149 (MQ=255)
                                                                                                      tCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGccc                                           >  4:261/1‑149 (MQ=255)
                                                                                                        ccGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCgg                                         >  6:227563/1‑149 (MQ=255)
                                                                                                         cGCTATTCTTATATTCAGCAGAGCTCTGACATCCAGGCGGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTGCGCCCGTGGCGAACGCGCTTACGACATCTACTGGCGCCTGTTGAAGGAGCgt                                                               <  6:218540/126‑1 (MQ=255)
                                                                                                         cGCAATTCTTATATTCAGCAGAGCTATGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGAGAACGCGCATACGACATCTAATCGCGCCTGTTGAAGCAGCgt                                                               >  5:218540/1‑126 (MQ=255)
                                                                                                           cAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTag                                      <  4:22138/149‑1 (MQ=255)
                                                                                                            aaTTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTaga                                     <  5:227563/149‑1 (MQ=255)
                                                                                                             aTTCTTATATTCAGCAGAGCTCTGACATCCTGTCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTTATCTTCCTGGTTGGCCCGGTagag                                    <  8:189813/149‑1 (MQ=255)
                                                                                                               tCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTcg                                                                                 >  3:158151/1‑102 (MQ=255)
                                                                                                               tCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTcg                                                                                 <  4:158151/102‑1 (MQ=255)
                                                                                                               tCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCACGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGa                                  >  5:30835/1‑149 (MQ=255)
                                                                                                                   atatTCAGCAGAGCTCTGACATCCAGGCCACAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTa                               >  4:140602/1‑148 (MQ=255)
                                                                                                                    tatTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACa                             >  5:169770/1‑149 (MQ=255)
                                                                                                                     atTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACAt                            >  8:200659/1‑149 (MQ=255)
                                                                                                                      ttCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACAt                            <  7:200659/148‑1 (MQ=255)
                                                                                                                      ttCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACAt                            <  5:182202/148‑1 (MQ=255)
                                                                                                                         agcagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATgcc                         <  6:35880/148‑3 (MQ=255)
                                                                                                                           cagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCaa                      <  4:88975/149‑1 (MQ=255)
                                                                                                                           cagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCaa                      <  3:62848/149‑1 (MQ=255)
                                                                                                                              agCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCATGTAGTCCTCTACCGGGCCAAcca                   <  5:145187/149‑2 (MQ=255)
                                                                                                                                   tGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCtcgtcg              >  7:306988/1‑149 (MQ=255)
                                                                                                                                     aCATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCATGTAGTCCTCTACCGGGCCAAccaggaa               <  5:308449/146‑6 (MQ=38)
                                                                                                                                         ccAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTgcg         >  6:13859/1‑148 (MQ=255)
                                                                                                                                          cAGGCCGCCTGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGtt            >  5:225283/1‑144 (MQ=255)
                                                                                                                                          cAGGCCGCCTGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGtt            <  6:225283/144‑1 (MQ=255)
                                                                                                                                          cAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTgcgc        >  2:106069/1‑148 (MQ=255)
                                                                                                                                          cAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTCTGATCTTCCTGGTTGGCACGGTAGAGGACTACATGGCCAACCTCGTCGTTgcgc        >  7:190117/1‑148 (MQ=255)
                                                                                                                                           aGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCaa      <  5:2764/149‑1 (MQ=255)
                                                                                                                                             gCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAAct    <  5:126832/149‑1 (MQ=255)
                                                                                                                                               cGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAActgc  >  5:64790/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAACTGC  >  NC_002947/2628240‑2628531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑