Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,628,387 G→T 6.3% G17C (GGC→TGC)  clpP → ATP‑dependent Clp protease proteolytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,628,3870GT 6.3% 244.6 / 4.8 79G17C (GGC→TGC) clpPATP‑dependent Clp protease proteolytic subunit
Reads supporting (aligned to +/- strand):  ref base G (37/36);  new base T (2/3);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

TGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAACTGC  >  NC_002947/2628241‑2628531
                                                                                                                                                  |                                                                                                                                                
tgAGTAACGGTCTTCTCTCGTAGGAAAACTCCACAAGCCAGCATTCGAGCTGGCTTGTGCGTATTAAAGCCATGACTATTTGTGAGTGAGAGCAGGACATGTCCCGCAATTCTTATTTTCAGCAGAGCTCTGAAATCCAGTCCGCAggc                                                                                                                                                >  3:356733/1‑149 (MQ=255)
    tAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGcc                                                                                                                                            <  3:297972/149‑1 (MQ=255)
         gTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCCTGCGGCCTggat                                                                                                                                       <  6:371000/149‑3 (MQ=255)
              ctctcGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGa                                                                                                                                   <  6:13273/148‑1 (MQ=255)
                 tcGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATg                                                                                                                                 <  1:177676/147‑1 (MQ=255)
                   gTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATgg                                                                                                                                <  8:194022/146‑1 (MQ=255)
                   gTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTa                                                                                                                             >  8:244282/1‑149 (MQ=255)
                     aGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATc                                                                                                                           >  6:126832/1‑149 (MQ=255)
                      ggAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCCTGCGGCCTggatgtca                                                                                                                                   >  1:48163/1‑134 (MQ=255)
                      ggAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCCTGCGGCCTggatgtca                                                                                                                                   <  2:48163/140‑7 (MQ=255)
                             tCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTc                                                                                                                   >  4:181652/1‑149 (MQ=255)
                               cacaAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGAt                                                                                                                                  <  6:297157/132‑1 (MQ=255)
                               cacaAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGAt                                                                                                                                  >  5:297157/1‑132 (MQ=255)
                                       agccTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGc                                                                                                         <  8:81114/149‑1 (MQ=255)
                                          cTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGaa                                                                                                      >  7:189813/1‑149 (MQ=255)
                                           ttCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATgg                                                                                                                                <  6:141242/122‑1 (MQ=255)
                                           ttCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAAc                                                                                                     >  7:43954/1‑149 (MQ=255)
                                           ttCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATACAGGCCGCAGGCGGCCTGGTCCCGATgg                                                                                                                                >  5:141242/1‑122 (MQ=255)
                                            tCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGtt                                                                                                                              <  2:317442/123‑1 (MQ=255)
                                            tCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGtt                                                                                                                              >  1:317442/1‑123 (MQ=255)
                                                    gCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTAc                                                                                             <  1:286722/148‑1 (MQ=255)
                                                     cTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGa                                                                                           <  3:167996/149‑1 (MQ=255)
                                                      ttGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGa                                                                                           >  2:164463/1‑148 (MQ=255)
                                                         tgCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGAc                                                                                          >  3:206534/1‑146 (MQ=255)
                                                         tgCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGAc                                                                                          <  4:206534/146‑1 (MQ=255)
                                                            gTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTAc                                                                                    >  6:2764/1‑149 (MQ=255)
                                                               ttCAAGCGATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTc                                                                                  <  5:114276/148‑1 (MQ=255)
                                                               ttCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTc                                                                                  <  4:189362/148‑1 (MQ=255)
                                                                tCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTcgc                                                                                >  3:22138/1‑149 (MQ=255)
                                                                 cAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTcgcg                                                                               >  5:324278/1‑149 (MQ=255)
                                                                  aaGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCTATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCgcgc                                                                              >  4:70887/1‑149 (MQ=255)
                                                                      cATGACTATTTGGGAGTGAGGGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGt                                                                          <  3:178746/149‑1 (MQ=255)
                                                                      cATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCTATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGt                                                                          <  3:70887/149‑1 (MQ=255)
                                                                       aTGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGtt                                                                         <  5:329581/149‑1 (MQ=255)
                                                                       aTGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGtt                                                                         >  8:31887/1‑149 (MQ=255)
                                                                         gACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTg                                                                        >  5:312941/1‑148 (MQ=255)
                                                                           cTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGaa                                                                                                      <  4:115616/116‑1 (MQ=255)
                                                                           cTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGaa                                                                                                      >  3:115616/1‑116 (MQ=255)
                                                                            tATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTg                                                                           >  4:368576/1‑142 (MQ=255)
                                                                            tATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTg                                                                           <  3:368576/142‑1 (MQ=255)
                                                                            tATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAg                                                                     >  5:35880/1‑148 (MQ=255)
                                                                              tttGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAg                                                                  >  6:182202/1‑149 (MQ=255)
                                                                              tttGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAg                                                                  >  5:42785/1‑149 (MQ=255)
                                                                                  ggAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCgtg                                                              <  7:31887/149‑1 (MQ=255)
                                                                                  ggAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCgt                                                               <  7:260398/148‑1 (MQ=255)
                                                                                  ggAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCgt                                                               >  8:260398/1‑148 (MQ=255)
                                                                                        aGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATc                                                         <  3:181652/148‑1 (MQ=255)
                                                                                        aGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCt                                                        >  2:364615/1‑149 (MQ=255)
                                                                                        aGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCt                                                        >  4:62848/1‑149 (MQ=255)
                                                                                                 cATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTg                                               <  6:324278/149‑1 (MQ=255)
                                                                                                    gTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGcc                                            >  4:60924/1‑149 (MQ=255)
                                                                                                     tCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGccc                                           >  4:261/1‑149 (MQ=255)
                                                                                                       ccGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCgg                                         >  6:227563/1‑149 (MQ=255)
                                                                                                        cGCTATTCTTATATTCAGCAGAGCTCTGACATCCAGGCGGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTGCGCCCGTGGCGAACGCGCTTACGACATCTACTGGCGCCTGTTGAAGGAGCgt                                                               <  6:218540/126‑1 (MQ=255)
                                                                                                        cGCAATTCTTATATTCAGCAGAGCTATGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGAGAACGCGCATACGACATCTAATCGCGCCTGTTGAAGCAGCgt                                                               >  5:218540/1‑126 (MQ=255)
                                                                                                          cAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTag                                      <  4:22138/149‑1 (MQ=255)
                                                                                                           aaTTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTaga                                     <  5:227563/149‑1 (MQ=255)
                                                                                                            aTTCTTATATTCAGCAGAGCTCTGACATCCTGTCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTTATCTTCCTGGTTGGCCCGGTagag                                    <  8:189813/149‑1 (MQ=255)
                                                                                                              tCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTcg                                                                                 >  3:158151/1‑102 (MQ=255)
                                                                                                              tCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTcg                                                                                 <  4:158151/102‑1 (MQ=255)
                                                                                                              tCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCACGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGa                                  >  5:30835/1‑149 (MQ=255)
                                                                                                                  atatTCAGCAGAGCTCTGACATCCAGGCCACAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTa                               >  4:140602/1‑148 (MQ=255)
                                                                                                                   tatTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACa                             >  5:169770/1‑149 (MQ=255)
                                                                                                                    atTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACAt                            >  8:200659/1‑149 (MQ=255)
                                                                                                                     ttCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACAt                            <  7:200659/148‑1 (MQ=255)
                                                                                                                     ttCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACAt                            <  5:182202/148‑1 (MQ=255)
                                                                                                                        agcagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATgcc                         <  6:35880/148‑3 (MQ=255)
                                                                                                                          cagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCaa                      <  4:88975/149‑1 (MQ=255)
                                                                                                                          cagAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCaa                      <  3:62848/149‑1 (MQ=255)
                                                                                                                             agCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCATGTAGTCCTCTACCGGGCCAAcca                   <  5:145187/149‑2 (MQ=255)
                                                                                                                                  tGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCtcgtcg              >  7:306988/1‑149 (MQ=255)
                                                                                                                                    aCATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCATGTAGTCCTCTACCGGGCCAAccaggaa               <  5:308449/146‑6 (MQ=38)
                                                                                                                                        ccAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTgcg         >  6:13859/1‑148 (MQ=255)
                                                                                                                                         cAGGCCGCCTGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGtt            >  5:225283/1‑144 (MQ=255)
                                                                                                                                         cAGGCCGCCTGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGtt            <  6:225283/144‑1 (MQ=255)
                                                                                                                                         cAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTgcgc        >  2:106069/1‑148 (MQ=255)
                                                                                                                                         cAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTCTGATCTTCCTGGTTGGCACGGTAGAGGACTACATGGCCAACCTCGTCGTTgcgc        >  7:190117/1‑148 (MQ=255)
                                                                                                                                          aGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCaa      <  5:2764/149‑1 (MQ=255)
                                                                                                                                            gCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAAct    <  5:126832/149‑1 (MQ=255)
                                                                                                                                              cGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAActgc  >  5:64790/1‑149 (MQ=255)
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TGAGTAACGGTCTTCTCTCGTAGGAAACCTCCACAAGCCAGCCTTCGAGCTGGCTTGTGCGTATTCAAGCCATGACTATTTGGGAGTGAGCGCAGGACATGTCCCGCAATTCTTATATTCAGCAGAGCTCTGACATCCAGGCCGCAGGCGGCCTGGTCCCGATGGTTATCGAGCAGTCCGCCCGTGGCGAACGCGCTTACGACATCTACTCGCGCCTGTTGAAGGAGCGTGTGATCTTCCTGGTTGGCCCGGTAGAGGACTACATGGCCAACCTCGTCGTTGCGCAACTGC  >  NC_002947/2628241‑2628531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑