Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,774,810	T→C	5.4%	P234P (CCA→CCG) ‡	PP_5521 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,774,810	0	T	C	5.4%	282.2 / 4.7	93	P234P (CCA→CCG) ‡	PP_5521	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (46/42);  new base C (3/2);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01

TCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGC  >  NC_002947/2774664‑2774954                                                                                                                                                   |                                                                                                                                                  tCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTgg                                                                                                                                                 >  4:64876/1‑149 (MQ=255)   cAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGgca                                                                                                                                               <  4:289905/149‑1 (MQ=255)    aGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTgg                                                                                                                                                 >  5:245988/1‑146 (MQ=255)    aGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTgg                                                                                                                                                 <  6:245988/146‑1 (MQ=255)          aGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGCCACAGCAGGca                                                                                                                                        <  2:308294/149‑2 (MQ=255)           gATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGa                                                                                                                                       <  3:77668/149‑1 (MQ=255)               tCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTc                                                                                                                                    <  2:257158/148‑1 (MQ=255)                 ggCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCa                                                                                                                                 >  1:71759/1‑149 (MQ=255)                 ggCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCa                                                                                                                                 >  2:357546/1‑149 (MQ=255)                   cTGCCTTGCTCACCCACGACGATCACCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCAcc                                                                                                                               >  6:66222/1‑149 (MQ=255)                     gCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGa                                                                                                                                       >  7:123188/1‑139 (MQ=255)                     gCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGa                                                                                                                                       <  8:123188/139‑1 (MQ=255)                      ccTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCtt                                                                                                                             <  8:89449/148‑1 (MQ=255)                      ccTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCt                                                                                                                              <  2:71759/147‑1 (MQ=255)                        ttGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAg                                                                                                                          >  7:126340/1‑149 (MQ=255)                          gCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCTGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTg                                                                                                                        >  1:303859/1‑149 (MQ=255)                          gCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGt                                                                                                                         <  1:357546/148‑1 (MQ=255)                              aCCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                      >  6:118764/1‑147 (MQ=255)                              aCCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                      >  1:371418/1‑147 (MQ=255)                               cccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                      >  1:112454/1‑146 (MQ=255)                               cccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                      <  2:112454/146‑1 (MQ=255)                                     acgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGaa                                                                                                             <  5:156320/149‑1 (MQ=255)                                     acgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCGGAGGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCCTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCCCCGTGAGTGGCCAGTACg                                                                                                               >  7:296144/1‑147 (MQ=255)                                      cgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAg                                                                                                            <  3:272933/149‑1 (MQ=255)                                         tCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATa                                                                                                         >  5:104010/1‑149 (MQ=255)                                           aGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATAcc                                                                                                       <  6:104010/149‑1 (MQ=255)                                            gCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCt                                                                                                      >  3:316814/1‑149 (MQ=255)                                            gCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCt                                                                                                      >  6:274448/1‑149 (MQ=255)                                               ggCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCg                                                                                                   <  7:253993/149‑1 (MQ=255)                                                gCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCgg                                                                                                  >  1:189720/1‑149 (MQ=255)                                                  cATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGgcg                                                                                                >  1:107170/1‑149 (MQ=255)                                                   aTCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTa                                                                                                                 >  7:35763/1‑131 (MQ=255)                                                   aTCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTa                                                                                                                 <  8:35763/131‑1 (MQ=255)                                                      cgccgACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTc                                                                                             <  5:118764/148‑1 (MQ=255)                                                       gccgACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCAc                                                                                           >  5:285332/1‑149 (MQ=255)                                                           acacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTAc                                                                                                                <  1:174194/124‑1 (MQ=255)                                                           acacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTAc                                                                                                                >  2:174194/1‑124 (MQ=255)                                                            cacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagc‑a                                                                                     <  4:72971/149‑1 (MQ=255)                                                            cacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagc‑a                                                                                     <  5:66222/149‑1 (MQ=255)                                                               cGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGACCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTa                                                                                  >  1:65029/1‑149 (MQ=255)                                                                    catgctccatgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGc                                                                             >  3:311453/1‑149 (MQ=255)                                                                           catgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGa                                                                                                                           <  1:212141/97‑1 (MQ=255)                                                                           catgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGa                                                                                                                           >  2:212141/1‑97 (MQ=255)                                                                           catgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCAt                                                                      >  5:184238/1‑149 (MQ=255)                                                                                tcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCggg                                                                  >  2:133491/1‑148 (MQ=255)                                                                                 cAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                      <  5:24641/96‑1 (MQ=255)                                                                                 cAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                      >  6:24641/1‑96 (MQ=255)                                                                                  aGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCgggg                                                                 >  4:131332/1‑147 (MQ=255)                                                                                  aGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGaa                                                               <  4:316814/149‑1 (MQ=255)                                                                                    ccccAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCCAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAgg                                                             >  4:23836/1‑149 (MQ=255)                                                                                    ccccAGGCTGATGTCGTCCGGGTCGGTGCCCTCAGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAgg                                                             >  2:348622/1‑149 (MQ=255)                                                                                       cAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGa                                                          >  2:225117/1‑149 (MQ=255)                                                                                       cAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGa                                                          >  5:132301/1‑149 (MQ=255)                                                                                        aGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGAt                                                         <  3:64876/149‑1 (MQ=255)                                                                                          gCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTgg                                                                          <  3:5555/130‑1 (MQ=255)                                                                                          gCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTgg                                                                          >  4:5555/1‑130 (MQ=255)                                                                                          gCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCa                                                       <  4:311453/149‑1 (MQ=255)                                                                                            tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgc                                                     >  8:163813/1‑149 (MQ=255)                                                                                            tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgc                                                     >  1:152115/1‑149 (MQ=255)                                                                                            tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgc                                                     >  1:157672/1‑149 (MQ=255)                                                                                            tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgc                                                     >  2:352595/1‑149 (MQ=255)                                                                                              aTGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgcg                                                    <  5:274448/148‑1 (MQ=255)                                                                                               tGTCGTCCGGGTCGGTGCCCTCAGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGcgcg                                                  <  1:348622/149‑1 (MQ=255)                                                                                                gtcgtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcgg                                                 >  8:325134/1‑149 (MQ=255)                                                                                                   gtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcggtcg                                              <  2:189720/149‑1 (MQ=255)                                                                                                        ggTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGtgtg                                         <  7:163813/149‑1 (MQ=255)                                                                                                         tgcGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTgg                                        <  1:133491/147‑1 (MQ=255)                                                                                                         gTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTgg                                        <  2:107170/149‑1 (MQ=255)                                                                                                           cGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGTGAAGGGGGTCAGCGCGGTCGGTGTGggc                                      <  3:23836/149‑1 (MQ=255)                                                                                                                  cTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGt                               >  2:189217/1‑149 (MQ=255)                                                                                                                  cTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGt                               >  6:310641/1‑149 (MQ=255)                                                                                                                   tCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTc                              >  5:164910/1‑149 (MQ=255)                                                                                                                   tCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTc                              >  5:234978/1‑149 (MQ=255)                                                                                                                     ggCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTc                              <  6:132301/147‑1 (MQ=255)                                                                                                                        cAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACg                         <  6:234978/149‑1 (MQ=255)                                                                                                                        cAGCTCTTCGAGCACGCGGGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGAt                                                         <  2:341750/117‑1 (MQ=255)                                                                                                                              ttCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATAGGGGAAGGGGATCAGCGCGCTCGGTGTGgccgg                                    >  4:58113/1‑132 (MQ=255)                                                                                                                                 gAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCGAGTACT‑GCTggtga                                                                       >  5:61715/1‑91 (MQ=255)                                                                                                                                 gAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCGAGTACT‑GCTggtga                                                                       <  6:61715/94‑4 (MQ=255)                                                                                                                                 gAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACg                <  7:325134/149‑1 (MQ=255)                                                                                                                                  aGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAGCGGCTCGAACgg               <  1:352595/149‑1 (MQ=255)                                                                                                                                  aGCAAGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACgg               >  6:190312/1‑149 (MQ=255)                                                                                                                                   gCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACggg              >  5:232252/1‑149 (MQ=255)                                                                                                                                   gCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACggg              >  5:188704/1‑149 (MQ=255)                                                                                                                                    cACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAATATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACggg              <  8:126340/148‑1 (MQ=255)                                                                                                                                        cgTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAAcc         <  5:310641/149‑1 (MQ=255)                                                                                                                                         tcgCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTacctt        >  3:300525/3‑145 (MQ=255)                                                                                                                                          cgCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTg       >  1:328771/2‑149 (MQ=255)                                                                                                                                           gCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCgggg                                                                 >  3:198244/1‑90 (MQ=255)                                                                                                                                           gCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCgggg                                                                 <  4:198244/90‑1 (MQ=255)                                                                                                                                             ctgctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcg    <  5:190312/149‑1 (MQ=255)                                                                                                                                                ctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcggc  <  2:371418/148‑1 (MQ=255)                                                                                                                                                ctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcggc  <  6:188704/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGC  >  NC_002947/2774664‑2774954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑