Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,774,812 G→A 5.6% P234S (CCA→TCA) ‡ PP_5521 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,774,8120GA 5.6% 278.1 / 3.5 89P234S (CCA→TCA) ‡PP_5521hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (44/40);  new base A (3/2);  total (47/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

TCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAACCC  >  NC_002947/2774664‑2774959
                                                                                                                                                    |                                                                                                                                                    
tCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTgg                                                                                                                                                      >  4:64876/1‑149 (MQ=255)
  cAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGgca                                                                                                                                                    <  4:289905/149‑1 (MQ=255)
   aGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTgg                                                                                                                                                      >  5:245988/1‑146 (MQ=255)
   aGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTgg                                                                                                                                                      <  6:245988/146‑1 (MQ=255)
         aGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGCCACAGCAGGca                                                                                                                                             <  2:308294/149‑2 (MQ=255)
          gATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGa                                                                                                                                            <  3:77668/149‑1 (MQ=255)
              tCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTc                                                                                                                                         <  2:257158/148‑1 (MQ=255)
                ggCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCa                                                                                                                                      >  1:71759/1‑149 (MQ=255)
                ggCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCa                                                                                                                                      >  2:357546/1‑149 (MQ=255)
                  cTGCCTTGCTCACCCACGACGATCACCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCAcc                                                                                                                                    >  6:66222/1‑149 (MQ=255)
                    gCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGa                                                                                                                                            >  7:123188/1‑139 (MQ=255)
                    gCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGa                                                                                                                                            <  8:123188/139‑1 (MQ=255)
                     ccTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCtt                                                                                                                                  <  8:89449/148‑1 (MQ=255)
                     ccTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCt                                                                                                                                   <  2:71759/147‑1 (MQ=255)
                       ttGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAg                                                                                                                               >  7:126340/1‑149 (MQ=255)
                         gCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCTGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTg                                                                                                                             >  1:303859/1‑149 (MQ=255)
                         gCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGt                                                                                                                              <  1:357546/148‑1 (MQ=255)
                             aCCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                           >  6:118764/1‑147 (MQ=255)
                             aCCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                           >  1:371418/1‑147 (MQ=255)
                              cccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                           >  1:112454/1‑146 (MQ=255)
                              cccACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                           <  2:112454/146‑1 (MQ=255)
                                    acgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGaa                                                                                                                  <  5:156320/149‑1 (MQ=255)
                                    acgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCGGAGGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCCTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCCCCGTGAGTGGCCAGTACg                                                                                                                    >  7:296144/1‑147 (MQ=255)
                                     cgaTCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAg                                                                                                                 <  3:272933/149‑1 (MQ=255)
                                        tCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATa                                                                                                              >  5:104010/1‑149 (MQ=255)
                                          aGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATAcc                                                                                                            <  6:104010/149‑1 (MQ=255)
                                           gCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCt                                                                                                           >  3:316814/1‑149 (MQ=255)
                                           gCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCt                                                                                                           >  6:274448/1‑149 (MQ=255)
                                              ggCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCg                                                                                                        <  7:253993/149‑1 (MQ=255)
                                               gCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCgg                                                                                                       >  1:189720/1‑149 (MQ=255)
                                                 cATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGgcg                                                                                                     >  1:107170/1‑149 (MQ=255)
                                                  aTCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTa                                                                                                                      >  7:35763/1‑131 (MQ=255)
                                                  aTCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTa                                                                                                                      <  8:35763/131‑1 (MQ=255)
                                                     cgccgACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTc                                                                                                  <  5:118764/148‑1 (MQ=255)
                                                      gccgACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCAc                                                                                                >  5:285332/1‑149 (MQ=255)
                                                          acacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTAc                                                                                                                     <  1:174194/124‑1 (MQ=255)
                                                          acacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTAc                                                                                                                     >  2:174194/1‑124 (MQ=255)
                                                           cacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagc‑a                                                                                          <  5:66222/149‑1 (MQ=255)
                                                           cacCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACcagc‑a                                                                                          <  4:72971/149‑1 (MQ=255)
                                                              cGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGACCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTa                                                                                       >  1:65029/1‑149 (MQ=255)
                                                                   catgctccatgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGc                                                                                  >  3:311453/1‑149 (MQ=255)
                                                                          catgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGa                                                                                                                                <  1:212141/97‑1 (MQ=255)
                                                                          catgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGa                                                                                                                                >  2:212141/1‑97 (MQ=255)
                                                                          catgctcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCAt                                                                           >  5:184238/1‑149 (MQ=255)
                                                                               tcAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCggg                                                                       >  2:133491/1‑148 (MQ=255)
                                                                                cAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                           <  5:24641/96‑1 (MQ=255)
                                                                                cAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGc                                                                                                                           >  6:24641/1‑96 (MQ=255)
                                                                                 aGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCgggg                                                                      >  4:131332/1‑147 (MQ=255)
                                                                                 aGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGaa                                                                    <  4:316814/149‑1 (MQ=255)
                                                                                   ccccAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCCAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAgg                                                                  >  4:23836/1‑149 (MQ=255)
                                                                                   ccccAGGCTGATGTCGTCCGGGTCGGTGCCCTCAGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAgg                                                                  >  2:348622/1‑149 (MQ=255)
                                                                                      cAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGa                                                               >  5:132301/1‑149 (MQ=255)
                                                                                      cAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGa                                                               >  2:225117/1‑149 (MQ=255)
                                                                                       aGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGAt                                                              <  3:64876/149‑1 (MQ=255)
                                                                                         gCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTgg                                                                               <  3:5555/130‑1 (MQ=255)
                                                                                         gCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTgg                                                                               >  4:5555/1‑130 (MQ=255)
                                                                                         gCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCa                                                            <  4:311453/149‑1 (MQ=255)
                                                                                           tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgc                                                          >  8:163813/1‑149 (MQ=255)
                                                                                           tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgc                                                          >  2:352595/1‑149 (MQ=255)
                                                                                           tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgc                                                          >  1:152115/1‑149 (MQ=255)
                                                                                           tGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgc                                                          >  1:157672/1‑149 (MQ=255)
                                                                                             aTGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAgcg                                                         <  5:274448/148‑1 (MQ=255)
                                                                                              tGTCGTCCGGGTCGGTGCCCTCAGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGcgcg                                                       <  1:348622/149‑1 (MQ=255)
                                                                                               gtcgtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcgg                                                      >  8:325134/1‑149 (MQ=255)
                                                                                                  gtcCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGcggtcg                                                   <  2:189720/149‑1 (MQ=255)
                                                                                                       ggTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGtgtg                                              <  7:163813/149‑1 (MQ=255)
                                                                                                        tgcGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTgg                                             <  1:133491/147‑1 (MQ=255)
                                                                                                        gTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTgg                                             <  2:107170/149‑1 (MQ=255)
                                                                                                          cGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGTGAAGGGGGTCAGCGCGGTCGGTGTGggc                                           <  3:23836/149‑1 (MQ=255)
                                                                                                                 cTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGt                                    >  6:310641/1‑149 (MQ=255)
                                                                                                                 cTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGt                                    >  2:189217/1‑149 (MQ=255)
                                                                                                                  tCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTc                                   >  5:234978/1‑149 (MQ=255)
                                                                                                                  tCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTc                                   >  5:164910/1‑149 (MQ=255)
                                                                                                                    ggCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTc                                   <  6:132301/147‑1 (MQ=255)
                                                                                                                       cAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACg                              <  6:234978/149‑1 (MQ=255)
                                                                                                                       cAGCTCTTCGAGCACGCGGGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGAt                                                              <  2:341750/117‑1 (MQ=255)
                                                                                                                             ttCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATAGGGGAAGGGGATCAGCGCGCTCGGTGTGgccgg                                         >  4:58113/1‑132 (MQ=255)
                                                                                                                                gAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCGAGTACT‑GCTggtga                                                                            >  5:61715/1‑91 (MQ=255)
                                                                                                                                gAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGCGAGTACT‑GCTggtga                                                                            <  6:61715/94‑4 (MQ=255)
                                                                                                                                gAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACg                     <  7:325134/149‑1 (MQ=255)
                                                                                                                                 aGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAGCGGCTCGAACgg                    <  1:352595/149‑1 (MQ=255)
                                                                                                                                 aGCAAGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACgg                    >  6:190312/1‑149 (MQ=255)
                                                                                                                                  gCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACggg                   >  5:188704/1‑149 (MQ=255)
                                                                                                                                  gCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACggg                   >  5:232252/1‑149 (MQ=255)
                                                                                                                                   cACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAATATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACggg                   <  8:126340/148‑1 (MQ=255)
                                                                                                                                       cgTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAAcc              <  5:310641/149‑1 (MQ=255)
                                                                                                                                        tcgCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTacctt             >  3:300525/3‑145 (MQ=255)
                                                                                                                                         cgCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTg            >  1:328771/2‑149 (MQ=255)
                                                                                                                                          gCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCgggg                                                                      >  3:198244/1‑90 (MQ=255)
                                                                                                                                          gCCTGCTGCCACAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCgggg                                                                      <  4:198244/90‑1 (MQ=255)
                                                                                                                                            ctgctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcg         <  5:190312/149‑1 (MQ=255)
                                                                                                                                               ctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcggc       <  2:371418/148‑1 (MQ=255)
                                                                                                                                               ctgTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTggcggc       <  6:188704/148‑1 (MQ=255)
                                                                                                                                                    gcagcaGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCgg                                                                                                       >  2:9317/1‑48 (MQ=255)
                                                                                                                                                    gcagcaGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAAccc  <  6:184238/148‑1 (MQ=255)
                                                                                                                                                    gcagcaGGCGAGTCGCACCTTCAGTGGCCAGTACGAAGATACCTTCgg                                                                                                       <  1:9317/48‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCCAGAACAAGATGTCGGCTGCCTTGCTCACCCACGACGATCAGCCGGCCATCCGCCGACACCGCACCATGCTCCATGCTCAGCCCCAGGCTGATGTCGTCCGGGTCGGTGCCCTCGGCCAGCTCTTCGAGCACGCGTGCCTGCTGTGGCAGCAGGCGAGTCGCACCTTGAGTGGCCAGTACGAAGATACCTTCGGCGCTCACCAGC‑AGTACTCGCTGGCCATCGGGGAAGGGGATCAGCGCGGTCGGTGTGGGCGGCAGGTCGAACGGCTCGAACGGGTAACCTGGCGGCAACCC  >  NC_002947/2774664‑2774959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑