Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,780,132 T→A 8.7% intergenic (+18/‑95) nfnB → / → PP_2433 6,7‑dihydropteridine reductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,780,1320TA 8.7% 127.8 / 2.7 46intergenic (+18/‑95)nfnB/PP_24336,7‑dihydropteridine reductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/20);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GGTTTGCGTGAGCGTGGGTTCACCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCG  >  NC_002947/2779989‑2780278
                                                                                                                                               |                                                                                                                                                  
ggTTTGCGTGAGCGTGGGTTCACCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCg                                                                                                                                                 <  2:17016/147‑1 (MQ=255)
    tGCGTGAGCGTGGGTTCACCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCt                                                                                                                                            <  6:250033/148‑1 (MQ=255)
      cGTGAGCGTGGGTTCACCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGc                                                                                                                                         <  2:284436/149‑1 (MQ=255)
               gggTTCACCAGTGTGGTGATACTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCTGCCCTGGCAATGCTGGc                                                                                                                                >  1:174418/1‑149 (MQ=255)
                 catCACCAGTGTCCTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATATCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCACAAGTCTTCACCTTCCACTCAAGTTTATGGGCCAGCTTTGCTGCCCTGGCAATCCTGCCgg                                                                                                                              >  4:47574/3‑149 (MQ=255)
                     aCCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTgg                                                                                                                                          <  1:351636/133‑1 (MQ=255)
                     aCCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTgg                                                                                                                                          >  2:351636/1‑133 (MQ=255)
                     aCCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCaa                                                                                                                          >  7:140154/1‑149 (MQ=255)
                     aCCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCAGCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGAAATGCTGGCGGTCac                                                                                                                          >  6:92993/1‑148 (MQ=255)
                        aGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCt                                                                                                                       <  7:259715/149‑1 (MQ=255)
                          tgtgGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGTCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTgg                                                                                                                     <  6:88960/149‑1 (MQ=255)
                          tgtgGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTgg                                                                                                                     >  4:168878/1‑149 (MQ=255)
                              gtgATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCaa                                                                                                                  <  8:60462/148‑1 (MQ=255)
                                    cTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGCCGGACAACCTGGCAAAGCCCTg                                                                                                           >  4:20799/1‑149 (MQ=255)
                                     tGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTgg                                                                                                          >  7:225724/1‑149 (MQ=255)
                                       aGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGc                                                                                                         <  5:24688/148‑1 (MQ=255)
                                         ccTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCaa                                                                                                                                       <  2:200383/116‑1 (MQ=255)
                                         ccTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCaa                                                                                                                                       >  1:200383/1‑116 (MQ=255)
                                                    gCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTgg                                                                                                                     <  8:78908/123‑1 (MQ=255)
                                                       gCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCg                                                                                        <  8:140154/149‑1 (MQ=255)
                                                               gATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTg                                                                                >  1:142794/1‑149 (MQ=255)
                                                                 tttCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGc                                                                              <  3:168878/149‑1 (MQ=255)
                                                                   tCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCACAAGTGTTCTCCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCa                                                                             <  3:47574/148‑1 (MQ=255)
                                                                           ccatttaaCAAGTCGGGGTTGCCGGCATCGAATGTGTTCACCTTCGTCTGATGTTTTGGGGGCCCCTTGGCGGCCGTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTGCACCGCCGGACCCGGCAAACTGGCAATTACCggg                                                                    <  2:139769/143‑1 (MQ=255)
                                                                            ggCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGc                                                                   <  2:205391/149‑1 (MQ=255)
                                                                                  aCAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCaggtcg                                                             >  1:139769/1‑144 (MQ=255)
                                                                                             ttGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTTGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACGCGGCATATACCGCTTACTTGt                                                  <  2:174418/149‑1 (MQ=255)
                                                                                                ccGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCg                                                <  3:20799/148‑1 (MQ=255)
                                                                                                 cGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGc                                                          <  4:234767/137‑1 (MQ=255)
                                                                                                 cGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGc                                                          >  3:234767/1‑137 (MQ=255)
                                                                                                 cGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCgg                                               >  6:20485/1‑148 (MQ=255)
                                                                                                  ggCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCg                                                           >  8:197076/1‑135 (MQ=255)
                                                                                                  ggCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCg                                                           <  7:197076/135‑1 (MQ=255)
                                                                                                          aaGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCg                                      >  6:145031/1‑148 (MQ=255)
                                                                                                                           tGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgccatgc                    >  2:85238/1‑149 (MQ=255)
                                                                                                                           tGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCCGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgccatgc                    >  2:80131/1‑149 (MQ=255)
                                                                                                                            gAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCt                   >  5:143403/1‑149 (MQ=255)
                                                                                                                             aaGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCtt                  >  7:349194/1‑149 (MQ=255)
                                                                                                                              aGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCtt                  <  8:225724/148‑1 (MQ=255)
                                                                                                                               attgccagggCCGCAAAGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgccatg                     >  5:322033/8‑144 (MQ=255)
                                                                                                                               attgccagggCCGCAAAGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGcatgccatg                     <  6:322033/137‑1 (MQ=255)
                                                                                                                                tttttGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAg                              <  5:306227/134‑1 (MQ=255)
                                                                                                                                tttttGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAg                              >  6:306227/1‑134 (MQ=255)
                                                                                                                                 tgccagggCCGCAAAGCGCCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGcc               >  6:2555/6‑148 (MQ=255)
                                                                                                                                      gggCCGCAAAGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTg         <  5:2555/149‑1 (MQ=255)
                                                                                                                                        gCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCt       >  6:295607/1‑149 (MQ=255)
                                                                                                                                               tGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTcgcg  <  8:349194/147‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GGTTTGCGTGAGCGTGGGTTCACCAGTGTGGTGATCCTGAGCCTGGGTTACCGCAGCGAGGCGGATTTCAACGCCGGGCTGAACAAGTCGCGGTTGCCGGCATCGCAAGTGTTCACCTTCCTCTGAAGTTTTTGGGGCCGCTTTGCGGCCCTGGCAATGCTGGCGGTCAACCTGGCAAAGCCCTGGCTTCGACCGCCCGACCCGGCAAACTGGCAATGACCCGGCATATACCGCATACTTGTCGGCAAATGCCGAAAAGGAGCATGCCATGCTTGCCGAAGTGCTTCGCG  >  NC_002947/2779989‑2780278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑