Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,790,697 G→T 6.4% S284* (TCG→TAG)  sstT ← serine/threonine transporter SstT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,790,6970GT 6.4% 247.4 / 5.7 78S284* (TCG→TAG) sstTserine/threonine transporter SstT
Reads supporting (aligned to +/- strand):  ref base G (35/38);  new base T (3/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

GCGCACACTGCCGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCGGGTAGGGGTTGCGGCGGATCTTCCA  >  NC_002947/2790550‑2790845
                                                                                                                                                   |                                                                                                                                                    
gcgcACAATGCCGCTACCACGGAGAGCAGCTCGGCAGTCGTCATGTCCACCTGGATACACAGTGTATGCACTGCGGCCAGGGTCAGCACGGTAATTGTGATCGCCGCACCGTCCATGTTGTTGGTCGCGCCCAGCGGGATCGACACCGa                                                                                                                                                     <  5:312608/149‑1 (MQ=255)
 cgcACACTGCCGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAg                                                                                                                                                    <  6:23306/149‑1 (MQ=255)
  gcACACTGCCGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAg                                                                                                                                                    >  1:4937/1‑148 (MQ=255)
  gcACACTGCCGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAg                                                                                                                                                    >  3:282235/1‑148 (MQ=255)
    acacTGCCGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAg                                                                                                                                                 <  1:215739/149‑1 (MQ=255)
    acacTGCCGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAg                                                                                                                                                 >  6:200570/1‑149 (MQ=255)
      acTGCCGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCCCTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGgt                                                                                                                                               >  5:15282/1‑149 (MQ=255)
          ccGCTACCCCGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTCATGGTCTCGCGCAGCGGGATCGACACCGAGTAGGTGTCt                                                                                                                                           >  8:257282/1‑149 (MQ=255)
          ccGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGgtgt                                                                                                                                             <  2:152947/147‑1 (MQ=255)
          ccGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCt                                                                                                                                           >  1:316192/1‑149 (MQ=255)
            gCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTc                                                                                                                                         <  5:352123/149‑1 (MQ=255)
            gCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTc                                                                                                                                         <  2:4937/149‑1 (MQ=255)
                ccACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCAt                                                                                                                                       <  5:200570/147‑1 (MQ=255)
                 cACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCCCCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACCCCGCGGAGGGGTCTTCagg                                                                                                                                      >  8:327455/1‑145 (MQ=255)
                     cTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGcc                                                                                                                                <  2:316192/149‑1 (MQ=255)
                          cagcaCGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGc                                                                                                                           <  4:230165/149‑1 (MQ=255)
                            gcaCGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCTACTCGGTGTcgat                                                                                                                                         <  3:126317/133‑4 (MQ=255)
                            gcaCGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCTACTCGGTGTcgat                                                                                                                                         >  4:126317/1‑130 (MQ=255)
                               cGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTcc                                                                                                                      <  6:100208/149‑1 (MQ=255)
                                     tCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAAc                                                                                                                >  7:259759/1‑149 (MQ=255)
                                            gtacACCTGGATACCCATGGTATGCACTGCGGGCAGGGTCTGCACGGTTATGGTGATCGCCGCATGTGCGTTGTTGATGGTCGCGCGCAGCGGTATCGACTCCGAGTAGGTTTCTTGTTGCAGGCCAAGGCGTTCCGAAAATGCAAGGt                                                                                                         <  4:282235/146‑1 (MQ=255)
                                                 ccTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCAcc                                                                                                    >  6:275640/1‑149 (MQ=255)
                                                     gATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGa                                                                                                >  4:238299/1‑149 (MQ=255)
                                                     gATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGa                                                                                                <  7:11022/149‑1 (MQ=255)
                                                        aCCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGt                                                                                             >  4:123775/1‑149 (MQ=255)
                                                          ccAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGa                                                                                                                    >  4:72518/1‑124 (MQ=255)
                                                          ccAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGa                                                                                                                    <  3:72518/124‑1 (MQ=255)
                                                          ccAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTg                                                                                           >  3:260827/1‑149 (MQ=255)
                                                          ccAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTg                                                                                           >  1:221758/1‑149 (MQ=255)
                                                          ccAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTg                                                                                           >  8:111296/1‑149 (MQ=255)
                                                          ccAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGGTCACCGGGATGTTg                                                                                           >  6:306360/1‑149 (MQ=255)
                                                            aGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTggc                                                                                         >  6:355411/1‑149 (MQ=255)
                                                              ggTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCAt                                                                                                                                       >  2:344459/1‑101 (MQ=255)
                                                              ggTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCAt                                                                                                                                       <  1:344459/101‑1 (MQ=255)
                                                               gTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCTACTCGGTGTcgatcc                                                                                                                                       >  3:156714/1‑95 (MQ=255)
                                                               gTATGCACTGCGGCCAGGGTCAGAACGGTAATGGTGATCGCAGCACCGGCCATCTTGATGGTCGCGACCATCGTGATCGACAACGAGTAGTTGTCTTCATGCAGGACAAGGCGTTCCGACAACGCCACGTTCACCGGGATCTAggaggc                                                                                      >  4:189640/1‑149 (MQ=255)
                                                                     aCTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGtt                                                                                                                        >  3:347547/1‑109 (MQ=255)
                                                                     aCTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGtt                                                                                                                        <  4:347547/109‑1 (MQ=255)
                                                                           gCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGt                                                                          <  3:314212/149‑1 (MQ=255)
                                                                            ccAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGt                                                                          >  1:195770/1‑148 (MQ=255)
                                                                              aGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTgaa                                                                       <  2:195770/149‑1 (MQ=255)
                                                                                ggTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTgaag                                                                      >  1:19372/1‑148 (MQ=255)
                                                                                     gCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCg                                                                <  4:260827/149‑1 (MQ=255)
                                                                                      cACGGTAATGGTGATCGCCGCACCGGCCTTGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCgg                                                               <  7:111296/149‑1 (MQ=255)
                                                                                       aCGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCgg                                                               >  8:355793/1‑148 (MQ=255)
                                                                                          gttaTGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGTGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCTGTAAt                                                           <  3:189640/146‑1 (MQ=255)
                                                                                             aTGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGcc                                                        >  5:272459/1‑149 (MQ=255)
                                                                                                gTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGc                                                      <  5:275640/148‑1 (MQ=255)
                                                                                                    tCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCa                                                 <  3:238299/149‑1 (MQ=255)
                                                                                                    tCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCa                                                 <  6:15282/149‑1 (MQ=255)
                                                                                                     cgccgcACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCa                                                 <  7:257282/148‑1 (MQ=255)
                                                                                                           aCCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCgg                                                                            <  5:379106/115‑1 (MQ=255)
                                                                                                           aCCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCgg                                                                            >  6:379106/1‑115 (MQ=255)
                                                                                                               gCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCAcag                                      <  2:221758/149‑1 (MQ=255)
                                                                                                               gCCATGTTGATGGTCGCGCCCAGCGGGATCAACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCg                                                                >  8:194300/1‑123 (MQ=255)
                                                                                                               gCCATGTTGATGGTCGCGCCCAGCGGGATCAACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCg                                                                >  8:188987/1‑123 (MQ=255)
                                                                                                               gCCATGTTGATGGTCGCGCCCAGCGGGATCAACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCg                                                                <  7:194300/123‑1 (MQ=255)
                                                                                                               gCCATGTTGATGGTCGCGCCCAGCGGGATCAACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCg                                                                <  7:188987/123‑1 (MQ=255)
                                                                                                                 cATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTgaa                                                                       >  5:353822/1‑114 (MQ=255)
                                                                                                                 cATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTgaa                                                                       <  6:353822/114‑1 (MQ=255)
                                                                                                                   tGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACagcagc                                  <  5:306360/149‑1 (MQ=255)
                                                                                                                       gATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGt                                >  2:233964/1‑147 (MQ=255)
                                                                                                                         tGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGc                            <  8:259759/149‑1 (MQ=255)
                                                                                                                         tGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGc                            <  2:19372/149‑1 (MQ=255)
                                                                                                                          ggTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGc                                                         <  3:7315/119‑1 (MQ=255)
                                                                                                                          ggTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGc                                                         >  4:7315/1‑119 (MQ=255)
                                                                                                                          ggTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCg                           >  4:164879/1‑149 (MQ=255)
                                                                                                                           gTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCgg                          <  3:123775/149‑1 (MQ=255)
                                                                                                                            tCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCAAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGc                            <  3:308526/146‑1 (MQ=255)
                                                                                                                            tCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCAAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGc                            >  4:308526/1‑146 (MQ=255)
                                                                                                                               cgcCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGGGAAGAATGCGGTACTGCCGCTTTCACGCAGGCACAGCAGCGTCAGCGGGTAg                      <  5:355411/149‑1 (MQ=255)
                                                                                                                                gcCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGc                                                                 <  5:140690/105‑1 (MQ=255)
                                                                                                                                gcCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGc                                                                 >  6:140690/1‑105 (MQ=255)
                                                                                                                                gcCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCGGGTAgg                     >  3:330512/1‑149 (MQ=255)
                                                                                                                                          gaagACACCTACTCGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCGGGTAGGGGTTgcggc            <  2:160180/145‑1 (MQ=255)
                                                                                                                                          gaagACACCTACTCGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCGGGTAGGGGTTgcggc            >  1:160180/4‑148 (MQ=255)
                                                                                                                                          agcGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCGGGTAGGGGTTgcggcg           <  8:172111/147‑1 (MQ=255)
                                                                                                                                              acacCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGTGGGTAGGGGTTGCGGCGGATc       >  6:58735/1‑149 (MQ=255)
                                                                                                                                                   gAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCGGGTAGGGGTTGCGGCGGATCTTCCa  <  4:330512/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGCACACTGCCGCTACCACGCTGAGCAGCACGGCAGTCGGCAGGTCCACCTGGATACCCAGGGTATGCACTGCGGCCAGGGTCAGCACGGTAATGGTGATCGCCGCACCGGCCATGTTGATGGTCGCGCCCAGCGGGATCGACACCGAGTAGGTGTCTTCATGCAGGCCAAGGCGTTCCGACAACGCCAGGTTCACCGGGATGTTGGCGGCCGAGCTGCGGGTGAAGAATGCGGTAATGCCGCTTTCACGCAGGCACAGCAGCGTCAGCGGGTAGGGGTTGCGGCGGATCTTCCA  >  NC_002947/2790550‑2790845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑