Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,793,755	A→C	18.6%	intergenic (+64/+63)	PP_2445 → / ← PP_2446	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,793,755	0	A	C	18.6%	99.4 / 17.1	43	intergenic (+64/+63)	PP_2445/PP_2446	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (15/20);  new base C (2/6);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CCATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGACGCC  >  NC_002947/2793609‑2793903                                                                                                                                                      |                                                                                                                                                     ccATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACt                                                                                                                                                    <  2:149500/149‑1 (MQ=255)  cATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTg                                                                                                                                                   >  4:263339/1‑149 (MQ=255)   aTGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTgg                                                                                                                                                  <  5:220471/149‑1 (MQ=255)         ttCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                            <  1:193185/149‑1 (MQ=255)            tcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTgg                                                                                                                                                  >  1:137660/1‑140 (MQ=255)            tcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTgg                                                                                                                                                  <  2:137660/140‑1 (MQ=255)             cGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                         >  2:5710/1‑148 (MQ=255)              gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                         <  6:53807/147‑1 (MQ=255)              gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                         <  3:970/147‑1 (MQ=255)              gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGa                                                                                                                                          <  3:123656/146‑1 (MQ=255)              gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATa                                                                                                                                       <  2:88726/149‑1 (MQ=255)              gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CGCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGa                                                                                                                                          <  4:221215/146‑1 (MQ=255)              gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGGTGATGTAAAAAG‑‑GGGCGCCTCGCGCCCTCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAAt                                                                                                                                        <  1:97210/149‑1 (MQ=37)               aCGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATAc                                                                                                                                      <  1:5710/149‑1 (MQ=255)                 ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                    <  6:268431/149‑1 (MQ=38)                 ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                    >  5:268431/1‑149 (MQ=38)                 ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                    <  4:103411/149‑1 (MQ=38)                 ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                    <  4:124530/149‑1 (MQ=38)                 ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCt                                                                                                                                    <  5:308621/149‑1 (MQ=255)                  gCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtg                                                                                                                                   <  3:263339/149‑1 (MQ=255)                    cgtCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtgtg                                                                                                                                 <  7:355821/147‑1 (MQ=255)                      tCGAGGTCCTCAACATCCTGGCCCGTGCTGCGCGTTTGAAGCGTGAGGCGGCTTAAGGTTTATTTAAAAAGCCTGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCtgt                                                                                                                                  <  1:114882/148‑1 (MQ=21)                      tCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtgtg                                                                                                                                 <  1:245460/147‑1 (MQ=255)                        gAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTg                                                                                                                                                   >  4:226831/1‑127 (MQ=255)                        gAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTg                                                                                                                                                   <  3:226831/127‑1 (MQ=255)                               tCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                            >  2:83324/1‑127 (MQ=255)                               tCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                            <  1:83324/127‑1 (MQ=255)                                                                                                                            gCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTg                            >  8:172484/1‑149 (MQ=255)                                                                                                                               cGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCgg                         <  7:172484/149‑1 (MQ=255)                                                                                                                                  tGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTgg                      >  5:119571/1‑149 (MQ=255)                                                                                                                                    cacaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGc                     <  6:119571/148‑1 (MQ=255)                                                                                                                                     acaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAg                   >  3:188703/1‑149 (MQ=255)                                                                                                                                       aGGTATTCGCGTTGCCAGTGCCACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGc                           >  8:352515/1‑139 (MQ=37)                                                                                                                                       aGGTATTCGCGTTGCCAGTGCCACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGc                           <  7:352515/139‑1 (MQ=37)                                                                                                                                       aGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                 >  7:106178/1‑149 (MQ=255)                                                                                                                                       aGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                 >  2:321035/1‑149 (MQ=255)                                                                                                                                       aGGTATTCGCGTGGCACTGGCAACGCGAAAACCTCTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                 >  2:253238/1‑149 (MQ=255)                                                                                                                                         gTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggtgg               >  6:368453/1‑149 (MQ=255)                                                                                                                                            ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGt                        <  6:256746/137‑1 (MQ=255)                                                                                                                                            ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGt                        >  5:256746/1‑137 (MQ=255)                                                                                                                                            ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGgtgt            <  8:106178/149‑1 (MQ=255)                                                                                                                                            ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGgtgt            >  4:96813/1‑149 (MQ=255)                                                                                                                                              cgcgTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGtgtg           >  6:85908/1‑148 (MQ=255)                                                                                                                                                      aCTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAcgcc  >  6:3205/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CCATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGACGCC  >  NC_002947/2793609‑2793903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑