Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,793,757 T→G 18.6% intergenic (+66/+61) PP_2445 → / ← PP_2446 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,793,7570TG18.6% 91.5 / 18.9 43intergenic (+66/+61)PP_2445/PP_2446membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (16/19);  new base G (2/6);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

CCATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGACGCCGC  >  NC_002947/2793609‑2793905
                                                                                                                                                       |                                                                                                                                                    
ccATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACt                                                                                                                                                      <  2:149500/149‑1 (MQ=255)
 cATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTg                                                                                                                                                     >  4:263339/1‑149 (MQ=255)
  aTGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTgg                                                                                                                                                    <  5:220471/149‑1 (MQ=255)
        ttCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                              <  1:193185/149‑1 (MQ=255)
           tcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTgg                                                                                                                                                    <  2:137660/140‑1 (MQ=255)
           tcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTgg                                                                                                                                                    >  1:137660/1‑140 (MQ=255)
            cGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                           >  2:5710/1‑148 (MQ=255)
             gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                           <  6:53807/147‑1 (MQ=255)
             gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                           <  3:970/147‑1 (MQ=255)
             gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGa                                                                                                                                            <  3:123656/146‑1 (MQ=255)
             gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATa                                                                                                                                         <  2:88726/149‑1 (MQ=255)
             gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CGCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGa                                                                                                                                            <  4:221215/146‑1 (MQ=255)
             gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGGTGATGTAAAAAG‑‑GGGCGCCTCGCGCCCTCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAAt                                                                                                                                          <  1:97210/149‑1 (MQ=37)
              aCGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATAc                                                                                                                                        <  1:5710/149‑1 (MQ=255)
                ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                      <  4:103411/149‑1 (MQ=38)
                ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                      <  6:268431/149‑1 (MQ=38)
                ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                      <  4:124530/149‑1 (MQ=38)
                ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                      >  5:268431/1‑149 (MQ=38)
                ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCt                                                                                                                                      <  5:308621/149‑1 (MQ=255)
                 gCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtg                                                                                                                                     <  3:263339/149‑1 (MQ=255)
                   cgtCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtgtg                                                                                                                                   <  7:355821/147‑1 (MQ=255)
                     tCGAGGTCCTCAACATCCTGGCCCGTGCTGCGCGTTTGAAGCGTGAGGCGGCTTAAGGTTTATTTAAAAAGCCTGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCtgt                                                                                                                                    <  1:114882/148‑1 (MQ=21)
                     tCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtgtg                                                                                                                                   <  1:245460/147‑1 (MQ=255)
                       gAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTg                                                                                                                                                     <  3:226831/127‑1 (MQ=255)
                       gAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTg                                                                                                                                                     >  4:226831/1‑127 (MQ=255)
                              tCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                              >  2:83324/1‑127 (MQ=255)
                              tCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                              <  1:83324/127‑1 (MQ=255)
                                                                                                                           gCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTg                              >  8:172484/1‑149 (MQ=255)
                                                                                                                              cGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCgg                           <  7:172484/149‑1 (MQ=255)
                                                                                                                                 tGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTgg                        >  5:119571/1‑149 (MQ=255)
                                                                                                                                   cacaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGc                       <  6:119571/148‑1 (MQ=255)
                                                                                                                                    acaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAg                     >  3:188703/1‑149 (MQ=255)
                                                                                                                                      aGGTATTCGCGTTGCCAGTGCCACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGc                             <  7:352515/139‑1 (MQ=37)
                                                                                                                                      aGGTATTCGCGTTGCCAGTGCCACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGc                             >  8:352515/1‑139 (MQ=37)
                                                                                                                                      aGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                   >  7:106178/1‑149 (MQ=255)
                                                                                                                                      aGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                   >  2:321035/1‑149 (MQ=255)
                                                                                                                                      aGGTATTCGCGTGGCACTGGCAACGCGAAAACCTCTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                   >  2:253238/1‑149 (MQ=255)
                                                                                                                                        gTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggtgg                 >  6:368453/1‑149 (MQ=255)
                                                                                                                                           ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGt                          <  6:256746/137‑1 (MQ=255)
                                                                                                                                           ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGt                          >  5:256746/1‑137 (MQ=255)
                                                                                                                                           ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGgtgt              >  4:96813/1‑149 (MQ=255)
                                                                                                                                           ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGgtgt              <  8:106178/149‑1 (MQ=255)
                                                                                                                                             cgcgTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGtgtg             >  6:85908/1‑148 (MQ=255)
                                                                                                                                                     aCTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAcgcc    >  6:3205/1‑149 (MQ=255)
                                                                                                                                                       tGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCCGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGAGGTGTGCATGAcgccgc  >  1:136512/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
CCATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGACGCCGC  >  NC_002947/2793609‑2793905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑