Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,793,761 A→C 20.5% intergenic (+70/+57) PP_2445 → / ← PP_2446 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,793,7610AC20.5% 83.5 / 17.5 39intergenic (+70/+57)PP_2445/PP_2446membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (14/17);  new base C (2/6);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGACGCCGCACGA  >  NC_002947/2793617‑2793909
                                                                                                                                                   |                                                                                                                                                    
ttCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                                  <  1:193185/149‑1 (MQ=255)
    cGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                               >  2:5710/1‑148 (MQ=255)
     gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                               <  6:53807/147‑1 (MQ=255)
     gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGaa                                                                                                                                               <  3:970/147‑1 (MQ=255)
     gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGa                                                                                                                                                <  3:123656/146‑1 (MQ=255)
     gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATa                                                                                                                                             <  2:88726/149‑1 (MQ=255)
     gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CGCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGa                                                                                                                                                <  4:221215/146‑1 (MQ=255)
     gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGGTGATGTAAAAAG‑‑GGGCGCCTCGCGCCCTCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAAt                                                                                                                                              <  1:97210/149‑1 (MQ=37)
      aCGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATAc                                                                                                                                            <  1:5710/149‑1 (MQ=255)
        ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                          >  5:268431/1‑149 (MQ=38)
        ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                          <  4:124530/149‑1 (MQ=38)
        ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                          <  4:103411/149‑1 (MQ=38)
        ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCt                                                                                                                                          <  6:268431/149‑1 (MQ=38)
        ggCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCt                                                                                                                                          <  5:308621/149‑1 (MQ=255)
         gCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtg                                                                                                                                         <  3:263339/149‑1 (MQ=255)
           cgtCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtgtg                                                                                                                                       <  7:355821/147‑1 (MQ=255)
             tCGAGGTCCTCAACATCCTGGCCCGTGCTGCGCGTTTGAAGCGTGAGGCGGCTTAAGGTTTATTTAAAAAGCCTGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGTGCCACGCGAATACCtgt                                                                                                                                        <  1:114882/148‑1 (MQ=21)
             tCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCtgtg                                                                                                                                       <  1:245460/147‑1 (MQ=255)
                      tCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                                  >  2:83324/1‑127 (MQ=255)
                      tCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAAcgc                                                                                                                                                  <  1:83324/127‑1 (MQ=255)
                                                                                                                   gCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTg                                  >  8:172484/1‑149 (MQ=255)
                                                                                                                      cGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCgg                               <  7:172484/149‑1 (MQ=255)
                                                                                                                         tGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTgg                            >  5:119571/1‑149 (MQ=255)
                                                                                                                           cacaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGc                           <  6:119571/148‑1 (MQ=255)
                                                                                                                            acaGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAg                         >  3:188703/1‑149 (MQ=255)
                                                                                                                              aGGTATTCGCGTTGCCAGTGCCACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGc                                 >  8:352515/1‑139 (MQ=37)
                                                                                                                              aGGTATTCGCGTTGCCAGTGCCACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGc                                 <  7:352515/139‑1 (MQ=37)
                                                                                                                              aGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                       >  2:321035/1‑149 (MQ=255)
                                                                                                                              aGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                       >  7:106178/1‑149 (MQ=255)
                                                                                                                              aGGTATTCGCGTGGCACTGGCAACGCGAAAACCTCTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt                       >  2:253238/1‑149 (MQ=255)
                                                                                                                                gTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggtgg                     >  6:368453/1‑149 (MQ=255)
                                                                                                                                   ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGt                              >  5:256746/1‑137 (MQ=255)
                                                                                                                                   ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGt                              <  6:256746/137‑1 (MQ=255)
                                                                                                                                   ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGgtgt                  <  8:106178/149‑1 (MQ=255)
                                                                                                                                   ttCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGgtgt                  >  4:96813/1‑149 (MQ=255)
                                                                                                                                     cgcgTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGtgtg                 >  6:85908/1‑148 (MQ=255)
                                                                                                                                             aCTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGAcgcc        >  6:3205/1‑149 (MQ=255)
                                                                                                                                               tGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCCGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGAGGTGTGCATGAcgccgc      >  1:136512/1‑149 (MQ=255)
                                                                                                                                                ggCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGACGCCGCa     <  5:368453/149‑1 (MQ=255)
                                                                                                                                                   aaCGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGACGCCGCACGa  >  2:270839/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAG‑‑GGGCGCCTCGCGCCC‑CTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTGGCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGACGCCGCACGA  >  NC_002947/2793617‑2793909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑