| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,810,593 | C→A | 6.6% | P334T (CCG→ACG) | thrS → | threonine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,810,593 | 0 | C | A | 6.6% | 195.5 / 3.8 | 61 | P334T (CCG→ACG) | thrS | threonine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base C (31/26); new base A (2/2); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01 | |||||||||||
GCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATG > NC_002947/2810456‑2810740 | gCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTg > 3:318287/1‑149 (MQ=255) ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAgg > 8:179560/1‑149 (MQ=255) gAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGACGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTGGCACGGGCAgttcat < 2:353928/149‑6 (MQ=255) gACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAgg < 5:134525/149‑1 (MQ=255) cGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTg < 5:139948/149‑1 (MQ=255) ggTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACAATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa < 2:323444/149‑1 (MQ=255) ttGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAAACGATGAACTGCCCCTGCCACGTGCAGGTGTTCAACAAGGGCATGAAAAg > 4:219587/1‑148 (MQ=255) gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCt < 3:32268/148‑1 (MQ=255) gACCGCATCCTGTGGGAGCGTTCCGGCCACTGCTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAcc < 6:270069/148‑1 (MQ=255) aCCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACcgc > 8:133761/1‑149 (MQ=255) aCCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACcgc > 2:354994/1‑149 (MQ=255) ccGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa < 6:354292/140‑1 (MQ=255) ccGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa > 5:354292/1‑140 (MQ=255) cATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGAcc > 3:344324/1‑149 (MQ=255) aTCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa > 5:233932/1‑136 (MQ=255) aTCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa < 6:233932/136‑1 (MQ=255) aTCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACcgc < 7:331062/144‑1 (MQ=255) aTCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACcgc > 8:331062/1‑144 (MQ=255) aTCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCt < 2:71776/149‑1 (MQ=255) aTCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCt < 4:318287/149‑1 (MQ=255) tgtgGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGt < 4:260895/99‑1 (MQ=255) tgtgGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGt > 3:260895/1‑99 (MQ=255) ggAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTg < 4:344324/148‑1 (MQ=255) ggAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGc > 6:358491/1‑149 (MQ=255) ttCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCg > 7:226925/1‑149 (MQ=255) cGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGcc > 1:129520/1‑148 (MQ=255) gTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtg > 1:116552/1‑149 (MQ=255) gTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGt < 7:179560/148‑1 (MQ=255) gTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGt < 1:354994/148‑1 (MQ=255) tCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCg < 1:306117/145‑1 (MQ=255) tCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCg > 2:306117/1‑145 (MQ=255) aCTATTCCGAAATCATGTTCACCACTTCGTCGGAAAGCCGTGTCTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTAAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc < 3:219587/149‑1 (MQ=255) cGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGt > 7:170291/1‑148 (MQ=255) gTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGATCGGTGCCTGCCACCGTAACGAGCCGt > 4:270172/1‑149 (MQ=255) caccacTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg > 2:247624/1‑149 (MQ=255) accacTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGcc < 4:53970/114‑1 (MQ=255) accacTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTACAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGcc > 3:53970/1‑114 (MQ=255) ccacTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg > 4:862/1‑147 (MQ=255) ccacTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg < 3:862/147‑1 (MQ=255) gtcgGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTg > 5:188731/1‑148 (MQ=255) ggAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGt < 5:98892/112‑1 (MQ=255) ggAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGt > 6:98892/1‑112 (MQ=255) aaaGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACg‑g > 4:80654/1‑148 (MQ=255) gcgGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTc > 6:113808/1‑119 (MQ=255) gcgGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTc < 5:113808/119‑1 (MQ=255) ggTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTcc < 5:295325/118‑1 (MQ=255) ggTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTcc > 6:295325/1‑118 (MQ=255) gTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCACGCACGCGCATGATGc‑cgtgcaggg < 5:358491/149‑5 (MQ=255) aCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcct < 3:206225/92‑1 (MQ=255) aCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcct > 4:206225/1‑92 (MQ=255) cGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCCGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTa > 4:114004/1‑149 (MQ=255) gATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGTGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGTCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTa > 8:252820/1‑148 (MQ=255) gATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGcc > 2:202245/1‑115 (MQ=255) gATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGcc < 1:202245/115‑1 (MQ=255) gATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTa > 8:12732/1‑148 (MQ=255) accTGCACGTGGCACGGGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGa > 8:75280/3‑149 (MQ=255) aaCTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAgg < 2:116552/148‑1 (MQ=255) cTGCACGTGGCACGGGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTc > 6:287533/1‑139 (MQ=255) cTGCACGTGGCACGGGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTc < 5:287533/139‑1 (MQ=255) gCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAAc < 4:124957/92‑1 (MQ=255) gCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAAc > 3:124957/1‑92 (MQ=255) cccGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATg > 3:103885/1‑149 (MQ=255) | GCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATG > NC_002947/2810456‑2810740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |