Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,921,460 G→A 100% G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA100.0% 119.5 / ‑6.8 37G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/16);  minor base T (0/1);  total (20/17)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAA  >  NC_002947/2921314‑2921602
                                                                                                                                                   |                                                                                                                                              
acacGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCagg                                                                                                                                              <  8:73276/149‑1 (MQ=255)
acacGGTGCGGGGCGAGGCCGGTAACCACACCCTCTACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCagg                                                                                                                                              <  8:39178/149‑1 (MQ=255)
     ttGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATCGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGc                                                                                                                                         >  4:129807/1‑149 (MQ=255)
          gggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGc                                                                                                                                     >  7:281274/1‑148 (MQ=255)
          gggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACACTGCAGGGCGGCGATGGTAACGACAAATTGTTCGGAGACGTGGGTACCGGCCCCCCTGATCagggagccag                                                                                                                                       >  2:93285/1‑141 (MQ=255)
              gAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGa                                                                                                                                >  4:162238/1‑149 (MQ=255)
                    ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGTCTTCAACGTCAATGACACCCTGCAGGGCGGCTATTGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGTGGCCGGCAACGACTTCCt                                                                                                                          <  8:281274/149‑1 (MQ=255)
                          gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                    >  7:24885/1‑149 (MQ=255)
                               ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCagg                                                                                                                                              >  2:251118/1‑118 (MQ=255)
                               ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCagg                                                                                                                                              <  1:251118/118‑1 (MQ=255)
                                   accGGGGTGGCTGGCGATGACAGCCGGGATGGCGGCAACGGCAATGACACCCTGCAGGGCTGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAg                                                                                                            <  8:352014/146‑1 (MQ=255)
                                   aaCGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAg                                                                                                            <  1:36996/148‑1 (MQ=255)
                                        gggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GCCACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTa                                                                                                         >  1:133710/1‑146 (MQ=255)
                                                  gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtggt                                                                                            >  1:236612/1‑149 (MQ=255)
                                                     gACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtggg                                                                                         >  1:7194/1‑149 (MQ=255)
                                                              gATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGcc                                                                                  <  3:129807/147‑1 (MQ=255)
                                                                  gcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACAGCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACTATACGGTGGTGGGTGGGGCCGGTAAc                                                                            <  8:24885/149‑1 (MQ=255)
                                                                      caacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGaca                                                                        <  2:7194/149‑1 (MQ=255)
                                                                        acggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGacac                                                                       >  4:346040/1‑148 (MQ=255)
                                                                          ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                    >  7:171072/1‑149 (MQ=255)
                                                                             aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGAt                                                                 <  3:162238/149‑1 (MQ=255)
                                                                             aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGAt                                                                 >  2:367830/1‑149 (MQ=255)
                                                                                gACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGc                                                              >  4:265869/1‑149 (MQ=255)
                                                                                   acacTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAAc                                                                                                       >  8:217140/4‑105 (MQ=255)
                                                                                   acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAAc                                                                                                       <  7:217140/105‑1 (MQ=255)
                                                                                     ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                          >  8:119041/1‑148 (MQ=255)
                                                                                     ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                          >  6:142040/1‑148 (MQ=255)
                                                                                          aGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAg                                                                                                            >  6:88122/1‑93 (MQ=255)
                                                                                                gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                              <  7:119041/149‑1 (MQ=255)
                                                                                                gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                              <  3:265869/149‑1 (MQ=255)
                                                                                                gccaaggTAACGCCACATTGTTCGGCGACGTGGGTACC‑GACCCCCTGACCTGGGTGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                              <  4:142412/144‑1 (MQ=255)
                                                                                                    tGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCTGTAACGACACCATGATCGCCACCGATGGAAACGATgtgt                                          <  8:171072/149‑1 (MQ=255)
                                                                                                      gTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                        <  3:346040/149‑1 (MQ=255)
                                                                                                         aCGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCATCGGTGCGGCAGGTAAGGATACGGTGGTCGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa                                      <  2:236612/148‑1 (MQ=255)
                                                                                                              acaTTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc                                >  1:148433/1‑149 (MQ=255)
                                                                                                               caTTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCt                               <  2:133710/149‑1 (MQ=255)
                                                                                                                aTTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCt                               >  4:220264/1‑148 (MQ=255)
                                                                                                                aTTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                              >  3:80232/1‑149 (MQ=255)
                                                                                                                          gACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTgg                    <  3:220264/149‑1 (MQ=255)
                                                                                                                             gTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCaa                 <  5:142040/149‑1 (MQ=255)
                                                                                                                                  tACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc            >  1:200489/1‑149 (MQ=255)
                                                                                                                                             cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAAAGATGTGTTCCAGTTCGCTGAAGGCTTTGGCAACGACCTGATCATCaa  >  6:312285/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAA  >  NC_002947/2921314‑2921602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑