Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,947,556 T→C 7.1% intergenic (‑35/‑177) yijP ← / → PP_2580 phosphoethanolamine transferase CptA/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,947,5560TC 7.1% 159.8 / 2.7 56intergenic (‑35/‑177)yijP/PP_2580phosphoethanolamine transferase CptA/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/26);  new base C (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

AGCAGCAGCGCCTGGGTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGCCGCCGGGGTATGATGGCGGCAG  >  NC_002947/2947412‑2947704
                                                                                                                                                |                                                                                                                                                    
agcagcagcGCCTGGGTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGGCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTgcgc                                                                                                                                                  >  1:106673/1‑149 (MQ=255)
agcagcagcGCCTGGGTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTgcgc                                                                                                                                                  <  6:304904/149‑1 (MQ=255)
  cagcagcGCCTGGGTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGt                                                                                                                                                <  8:43752/149‑1 (MQ=255)
    gcagcGCCTGGGTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTgg                                                                                                                                              >  8:66467/1‑149 (MQ=255)
    gcagcGCCTGGGTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTgg                                                                                                                                              <  7:103083/149‑1 (MQ=255)
    gcagcGCCTGGGTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTgg                                                                                                                                              <  8:141272/149‑1 (MQ=255)
               gTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGc                                                                                                                                   <  2:106673/149‑1 (MQ=255)
                     ccGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGC‑GGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGc                                                                                                                            <  5:34899/149‑1 (MQ=255)
                      cGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGc                                                                                                                            >  5:113757/1‑149 (MQ=255)
                       ggAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGc                                                                                                                            >  5:188979/1‑148 (MQ=255)
                        gagaAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTa                                                                                                                          <  6:113757/149‑1 (MQ=255)
                           aaaTACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATc                                                                                                                       >  3:145221/1‑149 (MQ=255)
                             aTACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTg                                                                                                                     <  6:188979/149‑1 (MQ=255)
                              tACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGc                                                                                                                    <  4:145221/149‑1 (MQ=255)
                                ccAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCgg                                                                                                                  >  8:197686/1‑149 (MQ=255)
                                          aGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGCGCGCGTGGCCGGAAAgg                                                                                                                                     <  7:336722/120‑1 (MQ=255)
                                          aGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGCGCGCGTGGCCGGAAAgg                                                                                                                                     >  4:71189/1‑120 (MQ=255)
                                          aGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGCGCGCGTGGCCGGAAAgg                                                                                                                                     <  3:71189/120‑1 (MQ=255)
                                          aGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGCGCGCGTGGCCGGAAAgg                                                                                                                                     >  8:336722/1‑120 (MQ=255)
                                               gagCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCtgtg                                                                                                            <  4:12705/140‑1 (MQ=255)
                                               gagCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCtgtg                                                                                                            >  3:12705/1‑140 (MQ=255)
                                                    agccGAGGCCGGCCCAGTCCACGGGCTTGTGCGCGGCGGGGGATGTTGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAAc                                                                                              <  4:50623/149‑1 (MQ=255)
                                                    agccGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGc                                                                                                                    <  4:291326/127‑1 (MQ=255)
                                                    agccGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGc                                                                                                                    >  3:291326/1‑127 (MQ=255)
                                                     gccGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAAcg                                                                                             >  4:171401/1‑149 (MQ=255)
                                                       cGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGc                                                                                                                    <  7:43442/124‑1 (MQ=255)
                                                       cGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGc                                                                                                                    >  8:43442/1‑124 (MQ=255)
                                                         aGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGtt                                                                                         <  3:318582/149‑1 (MQ=255)
                                                         aGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGtt                                                                                         <  2:183478/149‑1 (MQ=255)
                                                                       cACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGtt                                                                                         <  2:327851/135‑1 (MQ=255)
                                                                       cACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGtt                                                                                         >  1:327851/1‑135 (MQ=255)
                                                                                 tgCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGt                                                                 <  7:197686/149‑1 (MQ=255)
                                                                                      ggcggGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAg                                                            <  3:171401/149‑1 (MQ=255)
                                                                                             gATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCCGCATgg                                                                     <  4:9262/133‑1 (MQ=255)
                                                                                             gATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCCGCATgg                                                                     >  3:9262/1‑133 (MQ=255)
                                                                                                 tAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTg                                                  <  5:205072/148‑1 (MQ=255)
                                                                                                   gtgtgtGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGc                                               >  3:222103/1‑149 (MQ=255)
                                                                                                        tgACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGtt                                           <  7:66467/148‑1 (MQ=255)
                                                                                                          acacGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCAt                                        >  6:137258/1‑149 (MQ=255)
                                                                                                           cacGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGAGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATc                                       >  5:367113/1‑149 (MQ=255)
                                                                                                            ccGTATGCCTCGACGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCGAATCAGCGGGCTTTGAAAATAACCTCCACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATcg                                      >  8:257443/2‑149 (MQ=255)
                                                                                                                   ccTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTc                               <  6:367113/149‑1 (MQ=255)
                                                                                                                    cTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCa                                                         <  5:89736/122‑1 (MQ=255)
                                                                                                                    cTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCa                                                         >  6:89736/1‑122 (MQ=255)
                                                                                                                    cTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTcc                              >  7:32234/1‑149 (MQ=255)
                                                                                                                          gtACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTcgc                        >  5:237363/1‑149 (MQ=255)
                                                                                                                           tACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATc                                       >  7:193470/1‑133 (MQ=255)
                                                                                                                           tACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATc                                       <  8:193470/133‑1 (MQ=255)
                                                                                                                             ccTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTcgccgc                     >  3:140652/1‑149 (MQ=255)
                                                                                                                               ttGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGccgccg                   >  6:151373/1‑149 (MQ=255)
                                                                                                                               ttGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGccgccg                   >  5:97873/1‑149 (MQ=255)
                                                                                                                               ttGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGccgccg                   >  3:26437/1‑149 (MQ=255)
                                                                                                                               ttGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGccgccg                   >  1:228449/1‑149 (MQ=255)
                                                                                                                                tGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGCCGCCgg                  >  3:124994/1‑149 (MQ=255)
                                                                                                                                   cggcCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGTCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGCCGCCGGGGt               <  6:97873/149‑1 (MQ=255)
                                                                                                                                     gcCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGCCGCCGGGGTat             <  4:124994/149‑1 (MQ=255)
                                                                                                                                                tgCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGCCGCCGGGGTATGATGGCGGCAg  <  8:32234/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AGCAGCAGCGCCTGGGTTACACCGGAGAAATACCAGAAGAACAGCAAGAGCCAGCCGAGGCCGGCCCAGTCCACGCGCTTGTGCGCGGCGGGGGATGTAGTGTGTGACACGTATGCCTCGTCGTACCTTGGCGGCCAGCGGTTGTGCGCGTGGCCGGAAAGGGCGTGACGCTAATCTGCGGGCTGTGAAAATAACGTCAACGCGTTGAATCCTGTGACCTGCATGGCCGTAGCAGGCATACGCTGGGCCGTTCATCGCGTTTTCCCCTCGCCGCCGGGGTATGATGGCGGCAG  >  NC_002947/2947412‑2947704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑