Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,992,857	G→T	9.1%	A72A (GCC→GCA) ‡	PP_2616 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,992,857	0	G	T	9.1%	188.8 / 6.6	65	A72A (GCC→GCA) ‡	PP_2616	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base T (3/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

CCAGCAACTGAGCCGGCTCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCGGGTACTGGCTGTTGAA  >  NC_002947/2992709‑2993005                                                                                                                                                      |                                                                                                                                                        ccAGCAACTGAGCCGGCTCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCg                                                                                                                                                         <  4:313604/149‑1 (MQ=255)  cAGCAACTGAGCCGGCTCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCgg                                                                                                                                                        >  7:115098/1‑149 (MQ=255)  cAGCAACTGAGCCGGCTCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCgg                                                                                                                                                        >  1:45248/1‑149 (MQ=255)         tGAGCCGGCTCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAg                                                                                                                                                 >  8:287934/1‑149 (MQ=255)                 cTCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCTGGATGCCGCATCaccg                                                                                                                                         <  6:182596/149‑4 (MQ=255)                  tCGTAACCGTTCTGGCGGATTTCTTGCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCGAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAAGGGTGATGGGGCATCCAGCATCAGGtt                                                                                                                                        <  2:45248/149‑1 (MQ=255)                  tCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCTGGATGCCGCATCa                                                                                                                                            <  6:368629/145‑1 (MQ=255)                  tCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCTGGATGCCGCATCa                                                                                                                                            >  5:368629/1‑145 (MQ=255)                  tCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGtt                                                                                                                                        >  6:228020/1‑149 (MQ=255)                   cGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTg                                                                                                                                       >  1:179305/1‑149 (MQ=255)                       aCCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCg                                                                                                                                    <  5:228020/148‑1 (MQ=255)                                  gcATTTATTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTACGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGAAACAGGTTGGCGAACCCGC‑‑‑CCCg                                                                                                                      >  8:165300/1‑148 (MQ=255)                                       tcttcCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCt                                                                                                                 <  2:179305/148‑1 (MQ=255)                                          tcCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGaaa                                                                                                             >  5:24965/1‑149 (MQ=255)                                             tGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCTGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCGGAAATTc                                                                                                          >  3:301440/1‑149 (MQ=255)                                                 tgttgtCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCggg                                                                                                      <  8:115098/149‑1 (MQ=255)                                                   ttgtCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTATGGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGt                                                                                                     >  1:296567/1‑149 (MQ=255)                                                     gtCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAg                                                                                                  >  8:209617/1‑149 (MQ=255)                                                           cTGGCTGCGTACTTGCTGGTGCATGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCgggg                                                                                             >  2:124440/1‑148 (MQ=255)                                                              gCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTa                                                                                           <  5:261500/147‑1 (MQ=255)                                                              gCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGc                                                                                         >  8:158552/1‑149 (MQ=255)                                                                tGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCt                                                                                       >  3:6684/1‑149 (MQ=255)                                                                   gTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGATCAACGGTTATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCATCTGAAATTCCGGGTCAGCGGGGTAGCCTTc                                                                                     <  7:165300/148‑1 (MQ=255)                                                                   gTACTTGCGGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCg                                                                                    <  2:296567/149‑1 (MQ=255)                                                                       ttGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGaaa                                                                                >  1:69917/1‑149 (MQ=255)                                                                         gCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGTTCAGCGGGGTAGCCTTCGGaaa                                                                                <  7:287934/147‑1 (MQ=255)                                                                         gCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCAAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACg                                                                              >  1:290400/1‑149 (MQ=255)                                                                          cTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACgg                                                                             <  7:158552/149‑1 (MQ=255)                                                                          cTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACgg                                                                             <  6:24965/149‑1 (MQ=255)                                                                           tGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACggg                                                                            >  8:106620/1‑149 (MQ=255)                                                                           tGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACggg                                                                            >  1:328890/1‑149 (MQ=255)                                                                             gTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGaa                                                                                                                                  >  2:234612/1‑96 (MQ=255)                                                                             gTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGaa                                                                                                                                  <  1:234612/96‑1 (MQ=255)                                                                                cACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGctct                                                                        <  2:202115/148‑1 (MQ=255)                                                                                 aCGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTgg                                                                      >  3:259909/1‑149 (MQ=255)                                                                                   gTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCt                                                                    >  1:140302/1‑149 (MQ=255)                                                                                       tGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAgg                                                                >  1:78491/1‑149 (MQ=255)                                                                                        ggATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGt                                                               >  2:281391/1‑149 (MQ=255)                                                                                        ggATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACAAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGt                                                               >  3:118802/1‑149 (MQ=255)                                                                                         gATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGt                                                               <  2:69917/148‑1 (MQ=255)                                                                                                ggCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGt                                                       <  2:140302/149‑1 (MQ=255)                                                                                                     cTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATg                                                  >  2:87356/1‑149 (MQ=255)                                                                                                      tCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCg                                                                                    >  5:210742/1‑114 (MQ=255)                                                                                                      tCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCg                                                                                    <  6:210742/114‑1 (MQ=255)                                                                                                         tCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGt                                                               <  1:280657/132‑1 (MQ=255)                                                                                                         tCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGATGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGt                                                               >  2:280657/1‑132 (MQ=255)                                                                                                           cAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGaaa                                                                                >  4:315331/1‑113 (MQ=255)                                                                                                           cAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGaaa                                                                                <  3:315331/113‑1 (MQ=255)                                                                                                                 a‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGGATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTgg                                      >  7:39914/1‑149 (MQ=255)                                                                                                                                ccAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCgg                        >  6:84897/1‑149 (MQ=255)                                                                                                                                ccAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCgg                        >  7:218781/1‑149 (MQ=255)                                                                                                                                 cAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCggg                       <  7:106620/149‑1 (MQ=255)                                                                                                                                  aaGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCgggg                      <  4:6684/149‑1 (MQ=255)                                                                                                                                   aGCCGAACAACGGTGATGTGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCggggg                     >  7:115305/1‑149 (MQ=255)                                                                                                                                     ccGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCATGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGc                   <  2:290400/149‑1 (MQ=255)                                                                                                                                      cGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCg                  <  5:84897/149‑1 (MQ=255)                                                                                                                                      cGAACAACGGTGATGCGGCATCCAGCATCAGGGTGGCGAAGCCCCGTACTTGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCggggg                     <  1:124440/149‑1 (MQ=255)                                                                                                                                       gAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCgg                 <  4:259909/149‑1 (MQ=255)                                                                                                                                       gAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCgg                 <  6:278768/149‑1 (MQ=255)                                                                                                                                        aacaacGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGGTTGCCCATTGGTTGCTGCAGCCCGGGGGGCggg                <  1:281391/149‑1 (MQ=255)                                                                                                                                        aacaacGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCggg                <  7:209617/149‑1 (MQ=255)                                                                                                                                         acaacGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGT‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTgg                                      >  3:236631/1‑126 (MQ=255)                                                                                                                                         acaacGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGT‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTgg                                      <  4:236631/126‑1 (MQ=255)                                                                                                                                           caacctGATGCTGGATGCCGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTtgctg                                <  4:366795/125‑1 (MQ=255)                                                                                                                                           caacctGATGCTGGATGCCGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTtgctg                                >  3:366795/6‑130 (MQ=255)                                                                                                                                              ggTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCGGGTACTgg          <  2:328890/149‑1 (MQ=255)                                                                                                                                              ggTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCGGGTACTgg          <  2:78491/149‑1 (MQ=255)                                                                                                                                               gTGATGCGGCATCCAGGATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCGGGTACTGGc         <  8:39914/149‑1 (MQ=255)                                                                                                                                                      ggCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGc                                                                                         >  7:203229/1‑62 (MQ=255)                                                                                                                                                      ggCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGc                                                                                         <  8:203229/62‑1 (MQ=255)                                                                                                                                                      ggCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCGGGTACTGGCTGTTGaa  <  8:218781/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                        CCAGCAACTGAGCCGGCTCGTAACCGTGCTGGCGCATTTCTTCCTGAATGTTGTCGATCTGGCTGCGTACTTGCTGGTGCACGTCCTGGATGTTCGGCAGCTCGTCCAGCGTA‑CGCAGGCGTATGACCAAGCCGAACAACGGTGATGCGGCATCCAGCATCAGGTTGGCGAAGCCGC‑‑‑CCCGCAGCTGAAATTCCGGGTCAGCGGGGTAGCCTTCGGAAACGGGCTCTGGCTCAGGTTTGTCCGTGGATGCTTGCCCATTGGTTGCTGCAGCCCGGGGGGCGGGTACTGGCTGTTGAA  >  NC_002947/2992709‑2993005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑