Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,006,479 C→A 8.5% D1020E (GAC→GAA)  PP_2627 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,006,4790CA 8.5% 266.1 / 11.4 82D1020E (GAC→GAA) PP_2627membrane protein
Reads supporting (aligned to +/- strand):  ref base C (38/37);  new base A (4/3);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGCC  >  NC_002947/3006338‑3006622
                                                                                                                                             |                                                                                                                                               
tttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCtt                                                                                                                                           <  1:330270/148‑1 (MQ=255)
 ttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCtt                                                                                                                                           >  6:139996/1‑147 (MQ=255)
  tCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCTCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGa                                                                                                                                         <  3:325234/148‑1 (MQ=255)
  tCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCTTGa                                                                                                                                         <  5:139996/148‑1 (MQ=255)
   ccAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAgg                                                                                                                                       >  5:158659/1‑149 (MQ=255)
   ccAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAg                                                                                                                                        <  4:252213/148‑1 (MQ=255)
    cAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCTTGAgat                                                                                                                                      <  7:328600/149‑3 (MQ=255)
                           ccccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACggg                                                                                                                <  6:180713/148‑1 (MQ=255)
                           ccccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACgg                                                                                                                 <  7:181310/147‑1 (MQ=255)
                            cccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACgg                                                                                                                 >  3:369833/1‑146 (MQ=255)
                            cccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACgg                                                                                                                 <  4:369833/146‑1 (MQ=255)
                              cTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACTACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAAt                                                                                                            >  6:207342/1‑149 (MQ=255)
                              cTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAAt                                                                                                            >  5:151817/1‑149 (MQ=255)
                                  gtggtgAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCtt                                                                                                                                   >  4:53788/1‑122 (MQ=255)
                                  gtggtgAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCtt                                                                                                                                   <  3:53788/122‑1 (MQ=255)
                                  gtggtgAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATccc                                                                                                         <  6:151817/148‑1 (MQ=255)
                                                     cttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATc                                                                                     <  6:158659/149‑1 (MQ=255)
                                                      ttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATc                                                                                     >  8:232916/1‑148 (MQ=255)
                                                      ttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATc                                                                                     >  5:207884/1‑148 (MQ=255)
                                                         tttGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGag                                                                                  >  6:234458/1‑148 (MQ=255)
                                                           tGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGagca                                                                                <  6:53345/148‑1 (MQ=255)
                                                             gATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCt                                                                             >  2:317950/1‑149 (MQ=255)
                                                              aTGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGag                                                                                  >  8:91442/1‑143 (MQ=255)
                                                              aTGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGag                                                                                  <  7:91442/143‑1 (MQ=255)
                                                              aTGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCAGACGTGATCGAGCAGCTc                                                                            >  1:5574/1‑149 (MQ=255)
                                                                gACTTCGAGGCGTTCTTCGCGCAGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTATCCTGAAGGACAACCTTGATCCCTTGCAGTCCGCTCAGCACCGGCAATCCCTGATACGAGCCGACGTGATCGAGCAGCTc                                                                            >  8:102982/1‑147 (MQ=255)
                                                                 aCTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAg                                                                         <  8:139522/149‑1 (MQ=255)
                                                                   ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCt                                                                       >  1:275227/1‑149 (MQ=255)
                                                                   ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCt                                                                       >  7:10724/1‑149 (MQ=255)
                                                                   ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCt                                                                       >  8:107873/1‑149 (MQ=255)
                                                                     cGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCt                                                                       <  7:107873/147‑1 (MQ=255)
                                                                         gCGTTCTTCGGGCCGAAAGGGCGCCTGCAGNAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGa                                                                 >  1:31777/1‑149 (MQ=255)
                                                                           gTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgc                                                               >  3:356729/1‑149 (MQ=255)
                                                                            ttcttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgcg                                                              >  8:199792/1‑149 (MQ=255)
                                                                            ttcttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgcg                                                              >  4:293920/1‑149 (MQ=255)
                                                                              cttcGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgcgc                                                             <  4:356729/148‑1 (MQ=255)
                                                                                  gggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATcc                                                         <  1:331508/148‑1 (MQ=255)
                                                                                   ggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGt                                                                                                 >  7:258322/1‑107 (MQ=255)
                                                                                   ggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGt                                                                                                 <  8:258322/107‑1 (MQ=255)
                                                                                     ccGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTg                                                                                                                                  >  2:358241/1‑72 (MQ=255)
                                                                                     ccGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTg                                                                                                                                  <  1:358241/72‑1 (MQ=255)
                                                                                      cGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGaa                                                    <  3:293920/149‑1 (MQ=255)
                                                                                                 cTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                                      >  3:266119/1‑88 (MQ=255)
                                                                                                 cTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                                      <  4:266119/88‑1 (MQ=255)
                                                                                                    cagcagTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGAt                                        <  8:102533/147‑1 (MQ=255)
                                                                                                        agTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGt                                  <  5:207342/149‑1 (MQ=255)
                                                                                                               tGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGc                           <  2:31777/149‑1 (MQ=255)
                                                                                                                  tCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTaa                        <  7:102982/149‑1 (MQ=255)
                                                                                                                   cGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCg                                          >  5:105223/1‑130 (MQ=255)
                                                                                                                   cGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCg                                          <  6:105223/130‑1 (MQ=255)
                                                                                                                    ggTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttc                                              >  3:90574/1‑125 (MQ=255)
                                                                                                                    ggTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttc                                              <  4:90574/125‑1 (MQ=255)
                                                                                                                         ctcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCAGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCa                 <  1:317950/149‑1 (MQ=255)
                                                                                                                         ctcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                                      <  5:225129/64‑1 (MQ=21)
                                                                                                                         ctcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                                      >  6:225129/1‑64 (MQ=21)
                                                                                                                          tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAACCCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg                >  4:46008/1‑149 (MQ=255)
                                                                                                                          tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCttcttc                                           >  3:94851/1‑122 (MQ=255)
                                                                                                                          tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCttcttc                                           <  4:94851/122‑1 (MQ=255)
                                                                                                                          tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGtgtg                   <  3:149945/146‑1 (MQ=255)
                                                                                                                          tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGtgtg                   >  4:149945/1‑146 (MQ=255)
                                                                                                                          tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg                >  2:87026/1‑149 (MQ=255)
                                                                                                                          tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGAGCAg                >  6:289351/1‑149 (MQ=255)
                                                                                                                          tcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg                >  4:229884/1‑149 (MQ=255)
                                                                                                                           cAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt               >  5:151014/1‑149 (MQ=255)
                                                                                                                            aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttc                                              >  3:370950/1‑117 (MQ=255)
                                                                                                                            aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACcttc                                              <  4:370950/117‑1 (MQ=255)
                                                                                                                            aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAg                <  2:275227/147‑1 (MQ=255)
                                                                                                                            aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGtt              >  7:295726/1‑149 (MQ=255)
                                                                                                                            aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGtt              >  3:176503/1‑149 (MQ=255)
                                                                                                                            aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt               <  6:207884/148‑1 (MQ=255)
                                                                                                                            aaGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt               >  3:367473/1‑148 (MQ=255)
                                                                                                                              ggTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCa            <  8:295726/149‑1 (MQ=255)
                                                                                                                               gTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGc                  <  2:25137/142‑1 (MQ=255)
                                                                                                                               gTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGc                  >  1:25137/1‑142 (MQ=255)
                                                                                                                                     cTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATc       >  5:369420/1‑147 (MQ=255)
                                                                                                                                      tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGAt                                        >  7:45956/1‑113 (MQ=255)
                                                                                                                                      tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGAt                                        <  6:315524/113‑1 (MQ=255)
                                                                                                                                      tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGAt                                        >  5:315524/1‑113 (MQ=255)
                                                                                                                                      tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGAt                                        <  8:45956/113‑1 (MQ=255)
                                                                                                                                      tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTaa                        >  1:229142/1‑129 (MQ=255)
                                                                                                                                      tGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTaa                        <  2:229142/129‑1 (MQ=255)
                                                                                                                                        aaGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAg    <  5:234458/147‑1 (MQ=255)
                                                                                                                                             cAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGcc  >  3:54143/1‑144 (MQ=255)
                                                                                                                                             cAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGcc  <  4:54143/144‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
TTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCAGCATCGAGCC  >  NC_002947/3006338‑3006622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑