Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,008,318 C→T 11.5% S259S (TCG→TCA)  PP_2628 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,008,3180CT11.5% 136.1 / 7.4 52S259S (TCG→TCA) PP_2628ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base T (3/3);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

TCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGA  >  NC_002947/3008176‑3008460
                                                                                                                                               |                                                                                                                                               
tCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCgg                                                                                                                                           >  1:344639/1‑149 (MQ=255)
  aCCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCggg                                                                                                                                          >  8:169267/1‑148 (MQ=255)
  aCCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCggg                                                                                                                                          >  4:88757/1‑148 (MQ=255)
  aCCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCgg                                                                                                                                           <  2:344639/147‑1 (MQ=255)
   ccAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCa                                                                                                                                        >  4:309219/1‑149 (MQ=255)
    cAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCGTCGATGACCGGGcgg                                                                                                                                       <  8:254434/149‑3 (MQ=255)
    cAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCGTCGATGACCGGGcgg                                                                                                                                       <  7:119821/149‑3 (MQ=255)
     aGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCAtt                                                                                                                                      <  4:175189/149‑1 (MQ=255)
     aGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCAtt                                                                                                                                      <  6:314748/149‑1 (MQ=255)
      ggCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTg                                                                                                                                     <  2:81427/149‑1 (MQ=255)
      ggCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTg                                                                                                                                     <  3:115958/149‑1 (MQ=255)
        cgcATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGc                                                                                                                                   <  3:309219/149‑1 (MQ=255)
                            cTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTAGCCTGCTCCTGCCCGACCTTGc                                                                                                               <  7:237125/149‑1 (MQ=255)
                               cTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCa                                                                                                            >  2:111118/1‑149 (MQ=255)
                                ttGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAg                                                                                                           >  2:51645/1‑149 (MQ=255)
                                ttGAAATCTTCCATGGCTGCGCGCGCAAAGTGAGCTTCGCGGTTGGAGTGCGAAAACAGATTTACCCTGGTGATCTTGGTGTAAGCTTCAGAAACCCGCCCCG‑TCATCGACGACAGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCac                                                                                                           >  4:45985/1‑148 (MQ=255)
                                  gAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGc                                                                                                                                   >  7:149096/1‑123 (MQ=255)
                                  gAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGc                                                                                                                                   <  8:149096/123‑1 (MQ=255)
                                    aaTCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCgg                                                                                                       <  4:359275/149‑1 (MQ=255)
                                     aTCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCggg                                                                                                      <  4:258056/149‑1 (MQ=255)
                                        ttCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCTGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCgggg                                                                                                     <  7:17636/147‑1 (MQ=255)
                                        ttCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCTGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCgggg                                                                                                     >  8:17636/1‑147 (MQ=255)
                                          ccATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCt                                                                                                                           >  4:156385/1‑123 (MQ=255)
                                          ccATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCt                                                                                                                           <  3:156385/123‑1 (MQ=255)
                                            aTGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGaa                                                                                               <  1:51645/149‑1 (MQ=255)
                                                   cgcgcgcAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGCACATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACa                                                                                          <  1:111118/148‑1 (MQ=255)
                                                              tGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGACCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGcc                                                                             >  6:314708/1‑149 (MQ=255)
                                                                  cTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGGGTAAGCGTCAGAAACCCGCCCAG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACCGCATGGCCAAGCCGAAc                                                                         <  8:201768/149‑1 (MQ=255)
                                                                                  cGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCaa                                                         >  2:83381/1‑149 (MQ=255)
                                                                                       aCAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCTCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAg                                                    <  7:169267/149‑1 (MQ=255)
                                                                                       aCAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAg                                                    <  5:314708/149‑1 (MQ=255)
                                                                                           ttttACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAg                                                             >  4:96665/1‑136 (MQ=255)
                                                                                           ttttACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAg                                                             <  3:96665/136‑1 (MQ=255)
                                                                                             ttACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGgcag                                               <  2:298562/148‑1 (MQ=255)
                                                                                             ttACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGgcag                                               >  7:278809/1‑148 (MQ=255)
                                                                                               aCCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGgcagca                                             <  1:83381/148‑1 (MQ=255)
                                                                                                          gTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGACCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTAAggg                                >  6:30299/1‑149 (MQ=255)
                                                                                                           ttGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAggg                                >  8:158835/1‑148 (MQ=255)
                                                                                                           ttGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATAGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGTCAGCATCA‑GTTTCAggg                                >  5:172658/1‑148 (MQ=255)
                                                                                                                 tAAGAGTCAGAAACCCGCCCGG‑TCATCGACGACAGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGCAATACCGCATGGCCAAGCCGAACAGCACCACCCAGGCAATCAAGGGCACCATCC‑GTTTCAGGGCAAAgcc                         >  8:153867/1‑149 (MQ=255)
                                                                                                                       tCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGACTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGCGCCGCATCA‑GTTTCAGGGCAAAGCCGCCGGc                   >  2:215212/1‑149 (MQ=255)
                                                                                                                          gAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAg                >  8:83099/1‑149 (MQ=255)
                                                                                                                          gAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAg                <  8:278809/149‑1 (MQ=255)
                                                                                                                          gAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAg                >  6:305623/1‑149 (MQ=255)
                                                                                                                             caatgcccgG‑TCGTCGATGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAggg              >  1:297689/5‑148 (MQ=255)
                                                                                                                             caatgcccgG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAggg              >  1:81737/5‑148 (MQ=255)
                                                                                                                               ccgcccgG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAGGGCaa           >  3:134361/1‑149 (MQ=255)
                                                                                                                                tgcccgG‑TCGTCGATGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAGGGCAAt          >  8:295871/2‑149 (MQ=255)
                                                                                                                                  cccgG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGa        <  7:83099/149‑1 (MQ=255)
                                                                                                                                   ccgG‑TCGTCGATGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGGTTTCAGGGCAAAGCCGCCGGCCAGGGCaa           <  1:33250/146‑1 (MQ=255)
                                                                                                                                   ccgG‑TCGTCGATGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAGGGCaa           >  2:33250/1‑145 (MQ=255)
                                                                                                                                          aTCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGa  <  3:88757/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGG‑TCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCA‑GTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGA  >  NC_002947/3008176‑3008460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑