Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,010,966	G→A	8.8%	P9P (CCG→CCA)	bcsG →	endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,010,966	0	G	A	8.8%	189.1 / 7.2	69	P9P (CCG→CCA)	bcsG	endoglucanase
Reads supporting (aligned to +/- strand):  ref base G (34/28);  new base A (2/4);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

CCTGGCCTTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGG  >  NC_002947/3010818‑3011105                                                                                                                                                     |                                                                                                                                             cctggcctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCg                                                                                                                                              <  8:177922/149‑1 (MQ=255)       ctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTg                                                                                                                                        <  3:327289/149‑1 (MQ=255)       ctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTg                                                                                                                                        >  3:256486/1‑149 (MQ=255)       ctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGGTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTg                                                                                                                                        >  2:263684/1‑149 (MQ=255)          gttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGc                                                                                                                                      <  4:256486/148‑1 (MQ=255)           ttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGccc                                                                                                                                    >  3:353472/1‑149 (MQ=255)             gCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGc                                                                                                                                  >  7:90742/1‑149 (MQ=255)             gCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGc                                                                                                                                  >  2:48559/1‑149 (MQ=255)                     gcCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTgg                                                                                                                          >  4:28970/1‑149 (MQ=255)                        tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtacc                                                                                                                       >  3:40247/1‑143 (MQ=255)                            ttCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTgg                                                                                                                     >  3:180023/1‑147 (MQ=255)                            ttCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGa                                                                                                                   <  4:40247/149‑1 (MQ=38)                            ttCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGa                                                                                                                   <  6:211970/149‑1 (MQ=38)                                     aCGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTAGCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAAc                                                                                                          >  1:246489/1‑149 (MQ=255)                                        tGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctc                                                                                                       >  8:168752/1‑149 (MQ=255)                                               ttGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttt                                                                                                <  1:48559/149‑1 (MQ=255)                                                   gcCGTCGCCTT‑CCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGc                                                                                            >  6:193640/1‑148 (MQ=255)                                                      gTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaaa                                                                                         <  3:28970/149‑1 (MQ=255)                                                         gCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGtt                                                                                      <  4:353472/149‑1 (MQ=255)                                                         gCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGtt                                                                                      >  7:326527/1‑149 (MQ=255)                                                         gCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGtt                                                                                      >  2:245216/1‑149 (MQ=255)                                                            ttCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTct                                                                                   >  4:333638/1‑149 (MQ=255)                                                               ccccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTctgc                                                                                 <  1:245216/148‑1 (MQ=255)                                                                cccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTctgctg                                                                               <  7:49640/149‑1 (MQ=255)                                                                 ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGc                                                                                                                              >  5:104519/1‑101 (MQ=255)                                                                 ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGc                                                                                                                              <  6:104519/101‑1 (MQ=255)                                                                 ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTggg                                                                                                                    <  7:287398/111‑1 (MQ=255)                                                                 ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTggg                                                                                                                    >  8:287398/1‑111 (MQ=255)                                                                 ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTgg                                                                              >  5:102246/1‑149 (MQ=255)                                                                 ccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTgg                                                                              <  3:333638/149‑1 (MQ=255)                                                                  cccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGt                                                                             >  1:125511/1‑149 (MQ=255)                                                                          cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                     >  1:257436/1‑149 (MQ=255)                                                                          cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                     <  4:180023/149‑1 (MQ=255)                                                                           tGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTgg                                                                                                                     <  4:287204/100‑1 (MQ=255)                                                                           tGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTgg                                                                                                                     >  3:287204/1‑100 (MQ=255)                                                                           tGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                    >  8:192244/1‑149 (MQ=255)                                                                           tGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                    >  8:187053/1‑149 (MQ=255)                                                                            gAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                    >  7:29113/1‑148 (MQ=255)                                                                            gAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                    >  3:131365/1‑148 (MQ=255)                                                                                ccGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑c                                                              >  4:185998/1‑149 (MQ=255)                                                                                ccGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑c                                                              <  7:192244/149‑1 (MQ=255)                                                                                ccGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑c                                                              <  7:187053/149‑1 (MQ=255)                                                                                  gCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTc                                                            <  7:168752/149‑1 (MQ=255)                                                                                       tcGTACGCCTCCGCTCTTCACGCTATGAACCCCATAATTTCTAATACCATCTTCTGCCTCCCTGTACTGGCTCTCTGGCCTGTACTTGTATGGTTGAACCTCAATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCTACt                                                        <  2:332215/147‑1 (MQ=255)                                                                                            cgccgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAgg                                                  <  3:185998/149‑1 (MQ=255)                                                                                            cgccgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAg                                                   <  6:102246/148‑1 (MQ=255)                                                                                                  cgcgCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGc                                            <  2:125511/149‑1 (MQ=255)                                                                                                          aCGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCtg                                    <  2:257436/149‑1 (MQ=255)                                                                                                                     cccAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTg                                                                                                                      >  3:183617/1‑57 (MQ=255)                                                                                                                     cccAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTg                                                                                                                      <  4:183617/57‑1 (MQ=255)                                                                                                                              aCTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                 <  1:263684/149‑1 (MQ=255)                                                                                                                               cTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCtt               <  2:246489/149‑1 (MQ=255)                                                                                                                                tAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCt                   <  8:225815/144‑1 (MQ=255)                                                                                                                                tAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCt                   >  7:225815/1‑144 (MQ=255)                                                                                                                                 aaTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGc                         <  2:7250/137‑1 (MQ=255)                                                                                                                                 aaTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGc                         >  1:7250/1‑137 (MQ=255)                                                                                                                                  aTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGt             <  8:29113/148‑1 (MQ=255)                                                                                                                                      caccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                 <  8:45706/140‑1 (MQ=255)                                                                                                                                      caccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                 >  7:45706/1‑140 (MQ=255)                                                                                                                                      caccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCtt        >  8:327755/1‑149 (MQ=255)                                                                                                                                       accaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTc                                                                                    >  6:271292/1‑73 (MQ=255)                                                                                                                                       accaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTc                                                                                    <  5:271292/73‑1 (MQ=255)                                                                                                                                       accaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGttt                     >  4:319768/1‑135 (MQ=255)                                                                                                                                       accaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCttt       >  3:24325/1‑149 (MQ=255)                                                                                                                                          aTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAATGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTGGTTCGGTGCGTTTCTGCTTGTCCCTTTGcc    >  4:231939/1‑149 (MQ=255)                                                                                                                                           tCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCg   <  8:326527/149‑1 (MQ=255)                                                                                                                                            cACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCgg  >  2:109273/1‑149 (MQ=255)                                                                                                                                             aCCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                 <  6:17893/133‑1 (MQ=37)                                                                                                                                               ccTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCt                           >  2:249269/1‑121 (MQ=37)                                                                                                                                               ccTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCt                           <  1:249269/121‑1 (MQ=37)                                                                                                                                                     |                                                                                                                                             CCTGGCCTTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATG‑CTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGG  >  NC_002947/3010818‑3011105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑