Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,040,596	G→C	7.9%	G67R (GGC→CGC)	PP_2652 →	hydratase/decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,040,596	0	G	C	7.9%	154.9 / 3.9	51	G67R (GGC→CGC)	PP_2652	hydratase/decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base C (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

CTGGCGCAGCGGTGACCTGCTGACAGAACTGCCCGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGTGCTGGACCGGGATAT  >  NC_002947/3040451‑3040741                                                                                                                                                   |                                                                                                                                                   cTGGCGCAGCGGTGACCTGCTGACAGAACTGCCCGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCggcaggca                                                                                                                                                 >  7:120293/1‑149 (MQ=255)      gcAGCGGTGACCTGCTGACAGAACTGCCCGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGt                                                                                                                                            <  1:188757/149‑1 (MQ=255)             tGACCTGCTGACAGAACTGCCCGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGc                                                                                                                                      >  4:90849/1‑148 (MQ=255)                 ctgctgACAGAACTGCCCGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGtt                                                                                                                                 >  8:233884/1‑149 (MQ=255)                         aGAACTGCCCGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGt                                                                                                                                            <  5:209062/130‑1 (MQ=255)                         aGAACTGCCCGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGt                                                                                                                                            >  6:209062/1‑130 (MQ=255)                                 ccGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGCAGTCGGCAGCCCGGCACGCATGCCTGCCggg                                                                                                                                      <  1:33161/129‑2 (MQ=255)                                 ccGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGAtt                                                                                                                 <  8:120293/149‑1 (MQ=255)                                 ccGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGAtt                                                                                                                 <  3:249396/149‑1 (MQ=255)                                 ccGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCACGCATGCCTGCCggg                                                                                                                                      >  2:33161/1‑127 (MQ=255)                                  cGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCCCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTg                                                                                                                <  5:367219/149‑1 (MQ=255)                                   gCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGAtttg                                                                                                               >  6:220437/1‑147 (MQ=255)                                      aGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGcc                                                                                                             >  4:291909/1‑148 (MQ=255)                                         cTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGc                                                                                                                                          <  2:155095/116‑1 (MQ=255)                                          tCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGTCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTg                                                                                                        >  8:246851/1‑149 (MQ=255)                                            aaGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGc                                                                                                              <  3:253111/141‑1 (MQ=255)                                            aaGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGc                                                                                                              >  4:253111/1‑141 (MQ=255)                                                ccGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCg                                                                                                  >  7:322695/1‑149 (MQ=255)                                                 cGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGTCCTTGATTGGCCAACTGGAAGCg‑g                                                                                                <  7:233884/149‑1 (MQ=255)                                                 cGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTTGCCAACTGGAAGCg‑g                                                                                                <  5:220437/149‑1 (MQ=255)                                                 cGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCg‑g                                                                                                <  5:83736/149‑1 (MQ=255)                                                           aaaCAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCa                                                                                      <  6:138338/149‑1 (MQ=255)                                                                    tATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGc                                                                                   <  2:300051/143‑1 (MQ=255)                                                                    tATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGc                                                                                   >  1:300051/1‑143 (MQ=255)                                                                          aGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGcaca                                                                       >  5:163679/1‑149 (MQ=255)                                                                             cAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGc                                                                                                                         >  1:251081/1‑97 (MQ=255)                                                                             cAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGc                                                                                                                         <  2:251081/97‑1 (MQ=255)                                                                              atgACCAGGTGTTTGCCCCCGCCGCAGGCCATCGGGGCGGGTGGAAGCCGGGC‑GGCGGCATCCCGGCATGCATGCGTTCCGGGAATTTGTCGCCGCCCTTGATTGGCCAACTTGTAGCGCGGGCGTTGCCATGCCGATGGCGCACatat                                                                    <  1:27319/147‑1 (MQ=255)                                                                               ggACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATc                                                                   >  8:133705/1‑148 (MQ=255)                                                                                gACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCa                                                                 <  5:282253/149‑1 (MQ=255)                                                                                        gttTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAg                                                         >  8:231834/1‑149 (MQ=255)                                                                                             ccgctgccgcAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCg                                                       >  2:58269/1‑146 (MQ=255)                                                                                             ccgctgccgcAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCg                                                       <  1:58269/146‑1 (MQ=255)                                                                                             ccgctgccgcAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGt                                                     <  7:231834/148‑1 (MQ=255)                                                                                                   ccgcAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCg                                              >  8:252688/1‑149 (MQ=255)                                                                                                    cgcAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCgg                                             >  6:173317/1‑149 (MQ=255)                                                                                                            aGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATc                                     <  3:90849/149‑1 (MQ=255)                                                                                                            aGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATc                                     <  7:252688/149‑1 (MQ=255)                                                                                                             gcgcgcCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCCACTGGAAGCG‑GGGCGTTGCCCTGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCg                                    >  7:302217/1‑149 (MQ=255)                                                                                                              cgcgcCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGa                                   <  7:246851/149‑1 (MQ=255)                                                                                                               gcgcCGGTTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGACAGCGGTTAc                                                  <  7:156589/133‑1 (MQ=255)                                                                                                               gcgcCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGGCAGCGGTTAc                                                  >  8:156589/1‑133 (MQ=255)                                                                                                                     ggTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGa                             >  6:232200/1‑148 (MQ=255)                                                                                                                          aaGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGc                       >  2:100873/1‑149 (MQ=255)                                                                                                                          aaGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTAGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGc                       <  8:322695/149‑1 (MQ=255)                                                                                                                             cTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCtt                    >  5:159354/1‑149 (MQ=255)                                                                                                                                ggC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGt                 >  7:185320/1‑149 (MQ=255)                                                                                                                                 gC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGTg                <  7:133705/149‑1 (MQ=255)                                                                                                                                    gTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGTGCt              <  3:291909/149‑1 (MQ=255)                                                                                                                                       ggCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGTGCTGGa           <  6:159354/149‑1 (MQ=255)                                                                                                                                         cttgccGGCACGCATGCCTGCCGGCAAGTTCTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGTGCTGGAcc         >  1:28751/5‑149 (MQ=255)                                                                                                                                             ccGGCACGCATGCCTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGTGCTGGACCggg      <  2:28751/148‑1 (MQ=255)                                                                                                                                                gcaggcaTGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGTGCTGGACCGGGatat  <  6:163679/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CTGGCGCAGCGGTGACCTGCTGACAGAACTGCCCGCCAGCCTCAAGCCCGAGACGTTGAAACAAGGCTATGACAGCCAGGACCAGTTGTTTGCCGCTGCCGCAGGCCAGCGCGCCGGGTGGAAGCTGGGC‑GTCGGCAGCCCGGCAGGCATGCGTGCCGGCAAGTTGTCGCGGCCCTTGATTGGCCAACTGGAAGCG‑GGGCGTTGCCATGCCGATGGCGCACATATCCAGCTGCCAGCGGTTACGCCGGTGACCATCGAATGCGAAATTGCCTTCGTGCTGGACCGGGATAT  >  NC_002947/3040451‑3040741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑