Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,081,130	T→G	5.1%	H462P (CAT→CCT)	PP_2691 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,081,130	0	T	G	5.1%	234.3 / 2.2	73	H462P (CAT→CCT)	PP_2691	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base G (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCAGGTTGGCACGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCGGCAGCACTGAAGTCG  >  NC_002947/3080984‑3081273                                                                                                                                                    |                                                                                                                                                ccAGCAGGTTGGCACGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATgg                                                                                                                                               <  6:114552/149‑1 (MQ=255)    gcagGTTGGCACGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTAGGGCAc                                                                                                                                            >  8:351136/1‑149 (MQ=255)         ttGGCACGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCActgctg                                                                                                                                       >  6:301855/1‑149 (MQ=255)         ttGGCACGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCActgctg                                                                                                                                       >  7:201767/1‑149 (MQ=255)           ggCACGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAGAGTGCTCCAGCAGTGCCATAGCGCGctgctg                                                                                                                                       <  1:32770/148‑1 (MQ=255)              aCGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGTGCCATAGCGCGCTGCTGGa                                                                                                                                     <  4:317270/146‑1 (MQ=255)              aCGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGTGCCATAGCGCGCTGCTGGa                                                                                                                                     >  3:317270/1‑146 (MQ=255)                   tttCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCgg                                                                                                                             <  6:14245/149‑1 (MQ=255)                         cGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATa                                                                                                                       <  8:201767/149‑1 (MQ=255)                             gTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATAccc                                                                                                                    <  8:272784/148‑1 (MQ=255)                                    tttGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGGGGGCGATACGCAGCTGCa                                                                                                             <  6:2902/148‑1 (MQ=255)                                         cATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCtt                                                                                                       >  2:52579/1‑149 (MQ=255)                                          aTGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCttt                                                                                                      >  1:55440/1‑149 (MQ=255)                                          aTGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCAACCTGCAATTCttt                                                                                                      >  6:197674/1‑149 (MQ=255)                                             ccTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTc                                                                                                   <  1:329398/149‑1 (MQ=255)                                             ccTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTc                                                                                                   <  7:180504/149‑1 (MQ=255)                                              cTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCg                                                                                                  >  8:303465/1‑149 (MQ=255)                                                 gCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCggct                                                                                               >  6:39259/1‑149 (MQ=255)                                                   aGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCggctcc                                                                                             >  1:276037/1‑149 (MQ=255)                                                    ggcggcGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCggctcct                                                                                            <  2:55440/149‑1 (MQ=255)                                                    ggcggcGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATATCTTTTTCggctcct                                                                                            <  7:303465/149‑1 (MQ=255)                                                         cgTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCggctcctggctc                                                                                       <  5:301855/149‑1 (MQ=255)                                                                     ttGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCg                                                                                                                          <  3:93189/102‑1 (MQ=255)                                                                     ttGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCg                                                                                                                          >  4:93189/1‑102 (MQ=255)                                                                     ttGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAAt                                                                           >  6:166912/1‑149 (MQ=255)                                                                       gAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCggctcctg                                                                                           <  5:85994/131‑1 (MQ=255)                                                                       gAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCggctcctg                                                                                           >  6:85994/1‑131 (MQ=255)                                                                        aaCATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGcc                                                                        >  6:46201/1‑149 (MQ=255)                                                                           aTCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAg                                                                     >  5:105506/1‑149 (MQ=255)                                                                           aTCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCACTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAg                                                                     >  1:129645/1‑149 (MQ=255)                                                                                  gggCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATgg                                                                                                                                               <  4:58375/68‑1 (MQ=255)                                                                                  gggCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATgg                                                                                                                                               >  3:58375/1‑68 (MQ=255)                                                                                    gCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGctc                                                            <  5:46201/149‑1 (MQ=255)                                                                                      gATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGgcgc                                                                 >  5:106640/1‑142 (MQ=255)                                                                                      gATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGgcgc                                                                 <  6:106640/142‑1 (MQ=255)                                                                                      gATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCtctt                                                          >  7:18302/1‑149 (MQ=255)                                                                                        tCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCtcttct                                                        <  2:276037/149‑1 (MQ=255)                                                                                        tCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCtcttct                                                        >  3:311375/1‑149 (MQ=255)                                                                                          aGGAAGTGAGCGGGCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCttt                                                      <  1:52579/149‑1 (MQ=255)                                                                                            gAAGTGAGCGGTCAGCCCCTGATGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGGTTTGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTc                                                                                                   <  7:321088/102‑1 (MQ=255)                                                                                            gAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCttttt                                                    <  6:105506/149‑1 (MQ=255)                                                                                            gAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCttttt                                                    <  5:166912/149‑1 (MQ=255)                                                                                            gAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCtttt                                                     >  7:352144/1‑148 (MQ=255)                                                                                            gAAGTGAGCGCTCAGCCCCTGAGGCTGAAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTATTTTTc                                                                                                   >  8:321088/1‑102 (MQ=255)                                                                                              aGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCACTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCa                                                  <  2:129645/149‑1 (MQ=255)                                                                                                     ctgcAGCCTCAGGGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCa                                                  >  5:266335/4‑142 (MQ=255)                                                                                                     ctgcAGCCTCAGGGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCa                                                  <  6:266335/139‑1 (MQ=255)                                                                                                      gTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCg                                          <  4:311375/149‑1 (MQ=255)                                                                                                       tCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCgg                                         >  4:267155/1‑149 (MQ=255)                                                                                                        cAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGt                                        <  3:267155/149‑1 (MQ=255)                                                                                                          gCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTAc                                      <  7:351136/149‑1 (MQ=255)                                                                                                             ccTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTAc                                      >  7:11833/1‑146 (MQ=255)                                                                                                             ccTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTAc                                      >  1:251791/1‑146 (MQ=255)                                                                                                             ccTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTAc                                      <  2:251791/146‑1 (MQ=255)                                                                                                             ccTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCag                                   <  6:202323/149‑1 (MQ=255)                                                                                                                  ggCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAgaagaa                              <  8:18302/149‑1 (MQ=255)                                                                                                                   gCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGaagaag                             <  8:11833/149‑1 (MQ=255)                                                                                                                   gCTGCAGTTGCAG‑GTGCTACAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTAAGCTGAGCGGTACCAGaagaag                             >  5:216570/1‑149 (MQ=255)                                                                                                                    cTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGt                            <  5:39259/149‑1 (MQ=255)                                                                                                                      gCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGc                          >  4:314083/1‑149 (MQ=255)                                                                                                                      gCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCATGGGACATCTGGGCGAATGCCCCGGCCCGGGGGTCCTTTTGCAGGTCAGCGGTCCCGGATGAAGTg                           >  4:249356/1‑148 (MQ=255)                                                                                                                       cAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCATGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGc                          <  5:197674/148‑1 (MQ=255)                                                                                                                               g‑gTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCg                 >  3:187510/1‑149 (MQ=255)                                                                                                                                 gTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCgg                >  8:154703/1‑149 (MQ=255)                                                                                                                                   gCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCggctcct                                                                                            >  4:20829/1‑71 (MQ=255)                                                                                                                                   gCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCggctcct                                                                                            <  3:20829/71‑1 (MQ=255)                                                                                                                                   gCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCgg                >  6:261912/1‑147 (MQ=255)                                                                                                                                    cTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCGgcag             >  5:320599/1‑149 (MQ=255)                                                                                                                                        agcagcGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGGACCAGAAGAAGTGGTCCGCATCGgcaggcct         >  4:205681/1‑145 (MQ=255)                                                                                                                                           agcGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCGGCAGCACTGaa      <  5:261912/149‑1 (MQ=255)                                                                                                                                           agcGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCGGCAGCACTGaa      <  7:154703/149‑1 (MQ=255)                                                                                                                                              gcgcTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCGGCAGCACTGAAGTc   >  8:56089/1‑149 (MQ=255)                                                                                                                                               ggcgaTGGGACTGCTGGTTTTTGCGGGGGATACCCAGCTTCCTTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCGGCAGCACTGAAGTCg  <  3:249356/145‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                CCAGCAGGTTGGCACGGATTTCACCGTAGTTCGTTTTTGCCATGCCTTGCAGGCGGCGTACCGCGCCCTTGAACATCGGTTGGGCGATCAGGAAGTGAGCGGTCAGCCCCTGAGGCTGCAGTTGCAG‑GTGCTCCAGCAGCGCGCTATGGCACTGCTGGATATTGCGGGCGATACCCAGCTGCATTTCTTTTTCGGCTCCTGGCTCCTCTGCGCGAATGCCCAGGCGCGGCTCTTCTTTTTCAGCTGAGCGGTACCAGAAGAAGTGCTCCGCATCGGCAGCACTGAAGTCG  >  NC_002947/3080984‑3081273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑