Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,083,116 T→C 6.9% M318V (ATG→GTG)  PP_2692 ← BCCT family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,083,1160TC 6.9% 162.5 / 2.0 58M318V (ATG→GTG) PP_2692BCCT family transporter
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base C (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

ACAGCGTCATGATGATCTCGCGGATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGACAAAGCGGATGCCTTT  >  NC_002947/3082975‑3083263
                                                                                                                                             |                                                                                                                                                   
aCAGCGTCATGATGATCTCGCGGATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAgg                                                                                                                                              <  4:231329/149‑1 (MQ=255)
 cAGCGTCATGATGATCTCGCGGATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAggg                                                                                                                                             <  4:173862/149‑1 (MQ=255)
     gTCATGATGATCTCGCGGATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGAt                                                                                                                                         <  1:367394/149‑1 (MQ=255)
          gatgatCTCGCGGATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGa                                                                                                                                          <  4:147993/143‑1 (MQ=255)
          gatgatCTCGCGGATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGa                                                                                                                                          >  3:147993/1‑143 (MQ=255)
                   gcgGATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGt                                                                                                                           >  5:120314/1‑149 (MQ=255)
                      gATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTg                                                                                                                          >  6:105273/1‑147 (MQ=255)
                       aTGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAg                                                                                                                       <  6:120314/149‑1 (MQ=255)
                          gTGCGGCCCCGCGAGATCCGCGCGACATACAGGTCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAggg                                                                                                                     <  2:142157/148‑1 (MQ=255)
                             cGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCaaa                                                                                                                 <  8:95247/149‑1 (MQ=255)
                               gCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAgg                                                                                                               >  6:188527/1‑149 (MQ=255)
                                  ccGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGCACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACACTGTCAg                                                                                                                                               >  7:60810/1‑114 (MQ=255)
                                  ccGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACACTGTCAg                                                                                                                                               >  7:65886/1‑114 (MQ=255)
                                  ccGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACACTGTCAg                                                                                                                                               <  8:65886/114‑1 (MQ=255)
                                  ccGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACACTGTCAg                                                                                                                                               <  8:60810/114‑1 (MQ=255)
                                          tCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGaa                                                                                                     <  6:74136/148‑1 (MQ=255)
                                                 gACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGa                                                                                             <  1:240783/149‑1 (MQ=255)
                                                      aCAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGAt                                                                                         >  4:229219/1‑148 (MQ=255)
                                                         gggCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAAc                                                                                     <  5:188527/149‑1 (MQ=255)
                                                          ggCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGaa                                                                                      >  4:59630/1‑147 (MQ=255)
                                                             caccaTGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCg                                                                                 >  1:166424/1‑149 (MQ=255)
                                                             caccaTGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCg                                                                                 >  5:318962/1‑149 (MQ=255)
                                                             caccaTGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCCCGGAACATTGTCAGGGGGATGAAGTGCTACACGTGCAGGGGAAAg                                                                                                                >  2:283138/1‑118 (MQ=255)
                                                             caccaTGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCAACAGTCCAGACATTTCGGGTCGGCGCGGAACAGTGTCAGGGGGAGGAAGTGCTCCACTTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCg                                                                                 >  6:40108/1‑149 (MQ=255)
                                                                  tGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGAGGAAAATTGTCAGGGGGATGAAGTGCTCCAAGTGCAGGGCAAAGGCTTCCGGGAAACCGACGAGGATGACCAGCGTCTGt                                                                            >  7:131649/1‑149 (MQ=255)
                                                                     gcgcATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTcc                                                                          >  8:20820/1‑148 (MQ=255)
                                                                       gcATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGaa                                                                       >  6:270908/1‑149 (MQ=255)
                                                                          tAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATac                                                                    <  5:270908/149‑1 (MQ=255)
                                                                            gCCTATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGGCGGCGCGGAACATTGTCAGGTGGATGAAGTGCTCCACGTGAAGGGCAAAGGCTGCCGGGAATCCGCCGAGGATGAACAGCGTCGGTCCGAATacca                                                                  <  5:40108/149‑1 (MQ=255)
                                                                             ccGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATaccac                                                                 >  3:291836/1‑149 (MQ=255)
                                                                                    aCCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGaa                                                           >  2:87594/1‑148 (MQ=255)
                                                                                    aCCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGaa                                                           >  6:361433/1‑148 (MQ=255)
                                                                                     ccAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGaa                                                           >  8:64725/1‑147 (MQ=255)
                                                                                         ccccAATAGAACACCGTCCACCAGTCCATCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGc                                                     <  5:105273/149‑1 (MQ=255)
                                                                                          cccAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGc                                                     >  1:15842/1‑148 (MQ=255)
                                                                                           ccAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCTGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGt                                                       <  8:277527/145‑1 (MQ=255)
                                                                                           ccAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCGGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGt                                                       >  7:277527/1‑145 (MQ=255)
                                                                                                  aaCACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGat                                            <  8:33929/149‑1 (MQ=255)
                                                                                                    cacCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGatc                                           >  7:272555/1‑148 (MQ=255)
                                                                                                       cGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACa                                                                                    <  8:208003/104‑1 (MQ=255)
                                                                                                       cGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGAtcatca                                       <  7:20820/149‑1 (MQ=255)
                                                                                                         tCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAAc                                     <  3:229219/149‑1 (MQ=255)
                                                                                                         tCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAAc                                     <  3:59630/149‑1 (MQ=255)
                                                                                                         tCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAAc                                     >  1:58021/1‑149 (MQ=255)
                                                                                                            accaGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAAc                                     >  6:92558/1‑146 (MQ=255)
                                                                                                                 tCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGtt                             <  2:58021/149‑1 (MQ=255)
                                                                                                                   cAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGa                           <  2:166424/149‑1 (MQ=255)
                                                                                                                   cAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGa                           >  1:39775/1‑149 (MQ=255)
                                                                                                                    aGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGAt                          >  4:285445/1‑149 (MQ=255)
                                                                                                                      ccATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGAttt                        <  2:15842/149‑1 (MQ=255)
                                                                                                                             gggTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGTGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGa                  <  4:291836/148‑1 (MQ=255)
                                                                                                                             gggTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGa                  <  6:318962/148‑1 (MQ=255)
                                                                                                                                tCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGACa                >  7:295238/1‑147 (MQ=255)
                                                                                                                                     gcgGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGACAAAGCGGa         <  2:39775/149‑1 (MQ=255)
                                                                                                                                     gcgGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGACAAAGCGGa         >  4:295034/1‑149 (MQ=255)
                                                                                                                                      cgGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGACAAAGCGGAt        <  3:285445/149‑1 (MQ=255)
                                                                                                                                          aCATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGACAAAGCGGATGCCt    <  1:146201/149‑1 (MQ=255)
                                                                                                                                            aTTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCGAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGACAAAGCGGATGCCttt  >  4:96372/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
ACAGCGTCATGATGATCTCGCGGATGGTGCGGCCCCGCGAGATCCGCGCGACATACAGGGCCACCATGGGCGCATAGCCGATGAACCAGCCCCAATAGAACACCGTCCACCAGTCCAGCCATTTGGGGTCGGCGCGGAACATTGTCAGGGGGATGAAGTGCTCCACGTGCAGGGCAAAGGCTGCCGGGAAACCGCCGAGGATGAACAGCGTCGGTCCGAATACCACCATGAACAGTGCCAGGCCGATCATCAACCAGACGTTGATTTCGCTGACAAAGCGGATGCCTTT  >  NC_002947/3082975‑3083263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑