Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,104,299	T→A	5.8%	L276Q (CTG→CAG)	PP_2714 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,104,299	0	T	A	5.8%	212.5 / 2.4	69	L276Q (CTG→CAG)	PP_2714	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (29/35);  new base A (2/2);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

GCTGGCGCAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGCTGAACT  >  NC_002947/3104153‑3104443                                                                                                                                                    |                                                                                                                                                 gccgcagcAGATCGTCGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCCCGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTgg                                                                                                                                                >  6:87336/7‑149 (MQ=255) gCTGGCGCAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCt                                                                                                                                                  <  6:183445/147‑1 (MQ=255)       gcAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTc                                                                                                                                          >  7:55497/1‑149 (MQ=255)         aGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATTCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCtt                                                                                                                                        <  4:196647/149‑1 (MQ=255)         aGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCtt                                                                                                                                        <  4:280974/149‑1 (MQ=255)         aGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCtt                                                                                                                                        <  7:230731/149‑1 (MQ=255)         aGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCtt                                                                                                                                        <  8:228792/149‑1 (MQ=255)         aGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCAGGGCTTTCtg                                                                                                                                        <  1:37970/149‑2 (MQ=255)          gATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTtg                                                                                                                                       >  4:142999/1‑149 (MQ=255)            tCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTtgtg                                                                                                                                     >  1:31576/1‑149 (MQ=255)             cgacgaTCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTgg                                                                                                                                    >  2:8562/1‑149 (MQ=255)              gacgaTCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTggg                                                                                                                                   >  3:8539/1‑149 (MQ=255)                      gctgcGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTc                                                                                                                            >  6:374294/1‑148 (MQ=255)                       ctgcGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCgggg                                                                                                                              <  3:49161/145‑1 (MQ=255)                       ctgcGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCggg                                                                                                                               <  8:55497/144‑1 (MQ=255)                          cggcgTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATc                                                                                                                        <  5:189710/148‑1 (MQ=255)                                  aCATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGacac                                                                                                               <  1:8562/149‑1 (MQ=255)                                       gCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGggtg                                                                                                          <  2:31576/149‑1 (MQ=255)                                        cTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGggtgg                                                                                                         >  3:55412/1‑149 (MQ=255)                                            aTTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCaa                                                                                                     >  7:269326/1‑149 (MQ=255)                                             ttGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAAc                                                                                                    <  4:347574/149‑1 (MQ=255)                                                ccGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCgg                                                                                                                                <  4:364014/118‑1 (MQ=255)                                                ccGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAg                                                                                                 >  7:109413/1‑149 (MQ=255)                                                    tgctgcCCACGAAATGCGTACGCCTCTTGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTTGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGc                                                                                             <  2:298695/149‑1 (MQ=255)                                                       tgcCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACAGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCGGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCt                                                                                            >  1:91804/1‑148 (MQ=255)                                                          ccACGAAATGCGTACGCCTCTAGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTATTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCt                                                                                       >  8:56237/1‑149 (MQ=255)                                                           cACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGccc                                                                                        >  6:86829/1‑147 (MQ=255)                                                           cACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCtt                                                                                      >  7:106069/1‑149 (MQ=255)                                                            aCGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTg                                                                                     >  1:79521/1‑149 (MQ=255)                                                             cGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTgg                                                                                    <  8:269326/149‑1 (MQ=255)                                                              gAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGc                                                                                   <  4:268567/149‑1 (MQ=255)                                                                    cGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGt                                                                             >  2:11697/1‑149 (MQ=255)                                                                    cGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGt                                                                             <  8:109413/149‑1 (MQ=255)                                                                     gTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGc                                                                                                                     >  8:24335/1‑108 (MQ=255)                                                                     gTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGc                                                                                                                     <  7:24335/108‑1 (MQ=255)                                                                        cGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGa                                                                          <  5:374294/148‑1 (MQ=255)                                                                              tGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCaa                                                                     <  1:11697/147‑1 (MQ=255)                                                                                  cGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTgg                                                               <  3:142999/149‑1 (MQ=255)                                                                                  cGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTgg                                                               >  5:99109/1‑149 (MQ=255)                                                                                  cGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTgg                                                               >  8:266310/1‑149 (MQ=255)                                                                                  cGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTgg                                                               <  2:294642/149‑1 (MQ=255)                                                                                          gcCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGa                                                       <  7:145540/149‑1 (MQ=255)                                                                                           ccTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAg                                                      <  2:91804/149‑1 (MQ=255)                                                                                            cTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGc                                                     <  6:270956/149‑1 (MQ=255)                                                                                              gCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGc                                                     >  4:286886/1‑147 (MQ=255)                                                                                               cACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCTAGCCa                                                   <  5:87336/148‑1 (MQ=255)                                                                                               cACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCa                                                   <  4:55412/148‑1 (MQ=255)                                                                                                aCGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAg                                                  >  8:179416/1‑148 (MQ=255)                                                                                                  gcgcAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCAc                                               >  7:11650/1‑149 (MQ=255)                                                                                                  gcgcAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCAc                                               >  2:228428/1‑149 (MQ=255)                                                                                                  gcgcAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCAc                                               >  7:274982/1‑149 (MQ=255)                                                                                                             cgcTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGAc                                     <  6:99109/149‑1 (MQ=255)                                                                                                             cgcTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGAc                                     <  7:56237/149‑1 (MQ=255)                                                                                                             cgcTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGAc                                     <  8:106069/149‑1 (MQ=255)                                                                                                             cgcTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGGCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGAc                                     <  4:8539/149‑1 (MQ=255)                                                                                                                       cAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAg                            <  5:320519/148‑1 (MQ=255)                                                                                                                       cAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAg                            >  6:320519/1‑148 (MQ=255)                                                                                                                                 gCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAAc                                                                                                    >  7:312285/1‑66 (MQ=255)                                                                                                                                 gCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAAc                                                                                                    <  8:312285/66‑1 (MQ=255)                                                                                                                                   cGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGaaa               <  3:286886/149‑1 (MQ=255)                                                                                                                                   cGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGCGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGaaa               >  8:261846/1‑149 (MQ=255)                                                                                                                                    gAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAAc              <  7:266310/149‑1 (MQ=255)                                                                                                                                         caaGAAAGCCCAGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTgg         >  2:149919/3‑149 (MQ=255)                                                                                                                                          cAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGc        >  7:78157/1‑149 (MQ=255)                                                                                                                                          cAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGc        >  3:238087/1‑149 (MQ=255)                                                                                                                                          aaGAAAGCCCAGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTgg         <  2:341826/147‑1 (MQ=255)                                                                                                                                          aaGAAAGCCCAGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTgg         >  1:341826/2‑148 (MQ=255)                                                                                                                                                gCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGCTGAACt  <  2:79521/149‑1 (MQ=255)                                                                                                                                                  ccTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCtt                                                                                      >  2:308178/1‑63 (MQ=255)                                                                                                                                                  ccTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCtt                                                                                      <  1:308178/63‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GCTGGCGCAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAAC‑GCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGCTGAACT  >  NC_002947/3104153‑3104443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑