Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,143,200	G→A	6.3%	A42T (GCC→ACC)	rbsA‑II →	ribose ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,143,200	0	G	A	6.3%	193.9 / 2.0	63	A42T (GCC→ACC)	rbsA‑II	ribose ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (28/31);  new base A (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GTCAAACGCATCCGAGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGCTTGTT  >  NC_002947/3143054‑3143348                                                                                                                                                     |                                                                                                                                                     gTCAAACGCATCTGAGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGcc                                                                                                                                                    >  6:7237/1‑149 (MQ=255) gTCAAACGCATCCGAGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGcc                                                                                                                                                    >  6:77762/1‑149 (MQ=255)   cAAACGCATCCGAGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCgg                                                                                                                                                  >  8:350129/1‑149 (MQ=255)       cGCATCCGAGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCACCGGCAAg                                                                                                                                              >  7:330582/1‑149 (MQ=255)       cGCATCCGAGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCACCGGCAAg                                                                                                                                              >  7:268076/1‑149 (MQ=255)              gaggagACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGc                                                                                                                                       <  2:107981/149‑1 (MQ=255)              gAGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGc                                                                                                                                       >  5:378879/1‑149 (MQ=255)               aGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCt                                                                                                                                      <  6:378879/149‑1 (MQ=255)                 gCGACGCATGAGTACGCCGGTCCTGGAGCTGAGTGGCATTGTCAAGAACATCGGTTCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTCGCGGCGGGCAGCGTGCACGGTAGGGTGGGCGAGAACGGCGCCCGCAAGTCTACGCTGa                                                                                                                                    >  2:157675/1‑149 (MQ=255)                   gACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATc                                                                                                                                  <  7:350129/149‑1 (MQ=255)                    aCGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATc                                                                                                                                  >  1:93779/1‑148 (MQ=255)                      gCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAg                                                                                                                               <  1:189911/149‑1 (MQ=255)                        aTGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGt                                                                                                                             >  1:330024/1‑149 (MQ=255)                            gTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTg                                                                                                                         >  1:289867/1‑149 (MQ=255)                            gTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTg                                                                                                                         <  7:222550/149‑1 (MQ=255)                                gCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCg                                                                                                                     <  2:250799/149‑1 (MQ=255)                                gCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCg                                                                                                                     <  3:224931/149‑1 (MQ=255)                                gCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCg                                                                                                                     <  5:77762/149‑1 (MQ=255)                                    gTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCAt                                                                                                                 >  2:169757/1‑149 (MQ=255)                                           aGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGc                                                                                                          <  8:172406/149‑1 (MQ=255)                                                cGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCGTAAGCGGTAATAAATATGGAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGcc                                                                                                         <  1:304608/147‑1 (MQ=255)                                                 gTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTg                                                                                                                            >  6:202877/1‑125 (MQ=255)                                                 gTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTg                                                                                                                            <  5:202877/125‑1 (MQ=255)                                                    gCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGcc                                                                                                 <  2:330024/149‑1 (MQ=255)                                                          tCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTg                                                                                                                            <  5:71605/116‑1 (MQ=255)                                                          tCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTg                                                                                                                            >  6:71605/1‑116 (MQ=255)                                                          tCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGc                                                                                           <  2:289867/149‑1 (MQ=255)                                                            aaGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGcc                                                                                          <  6:36639/148‑1 (MQ=255)                                                            aaGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCct                                                                                         >  2:194120/1‑149 (MQ=255)                                                            aaGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCACCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGcc                                                                                          <  8:330582/148‑1 (MQ=255)                                                            aaGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCACCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGcc                                                                                          <  8:268076/148‑1 (MQ=255)                                                              gACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCctgc                                                                                       >  8:101545/1‑149 (MQ=255)                                                               aCCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCctgct                                                                                      <  7:101545/149‑1 (MQ=255)                                                               aCCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGGGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCctgct                                                                                      <  7:340985/149‑1 (MQ=255)                                                                 cTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCtgctgc                                                                                    >  6:69536/1‑149 (MQ=255)                                                                     ggTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGa                                                                                <  5:7237/149‑1 (MQ=255)                                                                              cGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGcc                                                                       >  4:329303/1‑149 (MQ=255)                                                                              cGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGcc                                                                       >  4:252023/1‑149 (MQ=255)                                                                               gggCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCg                                                                      >  5:8016/1‑149 (MQ=255)                                                                                   gcTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACg                                                                  <  1:169757/149‑1 (MQ=255)                                                                                     tCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACggg                                                                >  8:164639/1‑149 (MQ=255)                                                                                     tCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACggg                                                                >  1:366907/1‑149 (MQ=255)                                                                                       gACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACgg                                                                             <  4:164802/134‑1 (MQ=255)                                                                                       gACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACgg                                                                             >  3:164802/1‑134 (MQ=255)                                                                                       gACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGc                                                               <  1:194120/148‑1 (MQ=255)                                                                                       gACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCa                                                              >  6:54212/1‑149 (MQ=255)                                                                                         cGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCAtt                                                            <  5:69536/149‑1 (MQ=255)                                                                                             gcCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGcc                                                                                                 <  1:38932/108‑1 (MQ=255)                                                                                             gcCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGcc                                                                                                 >  2:38932/1‑108 (MQ=255)                                                                                             gcCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAg                                                        >  4:68390/1‑149 (MQ=255)                                                                                             gcCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAg                                                        >  5:358613/1‑149 (MQ=255)                                                                                                        cGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCa                                               <  6:8016/149‑1 (MQ=255)                                                                                                         gggTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAg                                              <  3:68390/149‑1 (MQ=255)                                                                                                          ggTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCTAGCCGCACGGGCATTTCAGCCCGCGCCAgg                                             <  1:222115/149‑1 (MQ=255)                                                                                                                             cACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTgg                          <  2:93779/149‑1 (MQ=255)                                                                                                                                      gtggGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCAt                    <  3:329303/146‑1 (MQ=255)                                                                                                                                      gtggGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATcca                 >  6:67819/1‑149 (MQ=255)                                                                                                                                       tggGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGc                         <  3:227701/140‑1 (MQ=255)                                                                                                                                       tggGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGc                         >  4:227701/1‑140 (MQ=255)                                                                                                                                            gagaACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAgg            >  4:272553/1‑148 (MQ=255)                                                                                                                                              gaACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAg                                        >  4:17878/1‑118 (MQ=255)                                                                                                                                              gaACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAg                                        <  3:17878/118‑1 (MQ=255)                                                                                                                                                aCGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGc       <  3:252023/149‑1 (MQ=255)                                                                                                                                                     gcCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGCttgtt  >  1:35650/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GTCAAACGCATCCGAGGCGACGCATGAGTACGCCGGTGCTGGAGCTGCGTGGCATTGTCAAGACCTTCGGTGCCACCCGGGCGCTCGACGGCGCCAGCCT‑‑GCGGGTGGCGGCGGGCAGCGTGCACGGTCTGGTGGGCGAGAACGGCGCCGGCAAGTCTACGCTGATCAAGGTGCTGGCCGGCATCCACCGCCCGGACGCCGGCAGCCTGCTGCTCGACGGCCAGCCGCACGGGCATTTCAGCCCGCGCCAGGTGGAGCGCCTGGGCATTGGCTTCATCCACCAGGAACGCTTGTT  >  NC_002947/3143054‑3143348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑