Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,154,866 A→G 6.9% E357G (GAA→GGA)  PP_2770 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,154,8660AG 6.9% 164.0 / 2.3 58E357G (GAA→GGA) PP_2770hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base G (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

GCGACCTGGCCGATCCGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAGTGGAAACTGATCAGCGAC  >  NC_002947/3154719‑3155014
                                                                                                                                                   |                                                                                                                                                    
gCGACCTGGCCGATCCGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGaa                                                                                                                                                     <  3:100304/149‑1 (MQ=255)
   aCCTGGCCGATCCGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAacac                                                                                                                                                  <  1:178363/149‑1 (MQ=255)
   aCCTGGCCGATCCGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAacac                                                                                                                                                  >  6:325095/1‑149 (MQ=255)
          cGATCCGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAAcc                                                                                                                                           >  3:163098/1‑149 (MQ=255)
          cGATCCGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAAcc                                                                                                                                           >  3:108956/1‑149 (MQ=255)
           gATCCGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCt                                                                                                                                          >  1:198977/1‑149 (MQ=255)
               cGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGAc                                                                                                                                      <  2:198977/149‑1 (MQ=255)
                              ccgtgtACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCTAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAg                                                                                                                       <  5:325095/147‑1 (MQ=255)
                              cGGTGTTCTACAACCTCGGCGTGGTCAACGGCATCCTCATCGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCATCCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAAGCTGGACGTTACCCGCCTGCAg                                                                                                                       <  6:45375/149‑1 (MQ=255)
                                 tgtACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCa                                                                                                                    <  3:285922/149‑1 (MQ=255)
                                    actacAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTc                                                                                                                 >  6:22238/1‑149 (MQ=255)
                                     ctacAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGGACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCg                                                                                                                >  5:367555/1‑149 (MQ=255)
                                     ctacAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGGACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCg                                                                                                                >  5:367552/1‑149 (MQ=255)
                                     ctacAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGAc                                                                                                                                      >  4:330752/1‑127 (MQ=255)
                                     ctacAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGAc                                                                                                                                      <  3:330752/127‑1 (MQ=255)
                                     ctacAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCg                                                                                                                >  8:41331/1‑149 (MQ=255)
                                         aaCCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGgcgc                                                                                                            <  7:41331/149‑1 (MQ=255)
                                           ccTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATAcc                                                                                                                                >  8:194486/1‑127 (MQ=255)
                                               ggCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAg                                                                                                        <  4:264446/147‑1 (MQ=255)
                                                 cGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGc                                                                                                    <  7:133102/149‑1 (MQ=255)
                                                 cGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGc                                                                                                    >  6:155509/1‑149 (MQ=255)
                                                    ggTCAACGGCATCCTCAACGGCCAGGCGTGGCGTCTGGCTCAGGAGACGTGCGCCAGCCAACCGCTGAGGGGCGAGCCGGTGCGTTGGGGCTTCCAACACCTGAACCTGGACGAAACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTgg                                                                                                 <  4:194987/149‑1 (MQ=255)
                                                     gTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTgg                                                                                                 <  7:69170/148‑1 (MQ=255)
                                                                  tCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGaaa                                                                                   >  8:177465/1‑149 (MQ=255)
                                                                    aaCGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGaaa                                                                                   <  4:163098/147‑1 (MQ=255)
                                                                          gAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAACGGGCTGGTGCAGCCGCTGAAACTATCCt                                                                            <  1:208780/148‑1 (MQ=255)
                                                                                   cGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTg                                                                                                  <  5:339755/117‑1 (MQ=255)
                                                                                   cGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTg                                                                                                  >  6:339755/1‑117 (MQ=255)
                                                                                         gCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAgg                                                            <  4:108956/149‑1 (MQ=255)
                                                                                           tCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCGGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCg                                                          >  1:18302/1‑149 (MQ=255)
                                                                                           tCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCg                                                          >  3:156318/1‑149 (MQ=255)
                                                                                           gcAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGGACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGGGCAGCCGCTGAACGTATCCTGCGCCGAGGATGAAGGCg                                                          >  8:355056/2‑149 (MQ=255)
                                                                                             aGGAGAAGTTCGGCAGGCAACCGCTGAGTGGAGAGCAGGAGCGTTGGGGCATCGAACACCTGAACCTCGACGATACCCGCCTGCAGGCACTCTGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGt                                                        >  4:127649/1‑149 (MQ=255)
                                                                                               gagaAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCt                                                                                       <  7:220522/116‑1 (MQ=255)
                                                                                               gagaAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCt                                                                                       >  8:220522/1‑116 (MQ=255)
                                                                                               gagaAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCACCTCCCATGAAGCCGTTgc                                                      >  2:131952/1‑148 (MQ=255)
                                                                                                     ttCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGt                                                <  4:124335/149‑1 (MQ=255)
                                                                                                       cGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTgc                                              >  1:179454/1‑149 (MQ=255)
                                                                                                        ggcaggcaACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTgcg                                             >  2:90557/1‑149 (MQ=255)
                                                                                                            ggcaACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCGGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTc                                         <  2:18302/149‑1 (MQ=255)
                                                                                                               aaCCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGGTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTAcag                                      >  1:25232/1‑149 (MQ=255)
                                                                                                               aaCCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCcag                                      <  5:155509/149‑1 (MQ=255)
                                                                                                                    cTGCGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCa                                                                  <  5:351137/116‑1 (MQ=255)
                                                                                                                    cTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCa                                                                  >  6:351137/1‑116 (MQ=255)
                                                                                                                    cTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTg                                 <  1:90557/149‑1 (MQ=255)
                                                                                                                     tGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTgg                                >  5:281716/1‑149 (MQ=255)
                                                                                                                          ggCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATc                                                                              >  2:318517/1‑98 (MQ=255)
                                                                                                                           gCGAGCAGGTGCGTTGGGGCTTCGGACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACggg                          <  6:367552/149‑1 (MQ=255)
                                                                                                                           gCGAGCAGGTGCGTTGGGGCGTCGGACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACggg                          <  6:367555/149‑1 (MQ=255)
                                                                                                                                 aGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTc                                         <  8:298225/128‑1 (MQ=255)
                                                                                                                                 aGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTc                                         >  7:298225/1‑128 (MQ=255)
                                                                                                                                 aGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAg                    >  5:119785/1‑149 (MQ=255)
                                                                                                                                  ggTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCTCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAGt                   <  4:156318/149‑1 (MQ=255)
                                                                                                                                  ggTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGAAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAGt                   <  6:139421/149‑1 (MQ=255)
                                                                                                                                     gCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAGTGGa                <  1:254007/149‑1 (MQ=255)
                                                                                                                                        ttGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTg                                 <  1:43261/129‑1 (MQ=255)
                                                                                                                                        ttGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTg                                 >  2:43261/1‑129 (MQ=255)
                                                                                                                                           gggCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAGTGGAAACTg           <  3:147806/148‑1 (MQ=255)
                                                                                                                                                  gAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAGTGGAAACTGATCAGCGa   >  6:12958/1‑149 (MQ=255)
                                                                                                                                                   aaCACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAGTGGAAACTGATCAGCGAc  >  3:77983/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGACCTGGCCGATCCGAAGCGCTTCGGCACGGTGTACTACAACCTCGGCGTGGTCAACGGCATCCTCAACGTCGAGGCGTTGCGTCTGGCTCAGGAGAAGTTCGGCAGGCAACCGCTGAGTGGCGAGCAGGTGCGTTGGGGCTTCGAACACCTGAACCTGGACGATACCCGCCTGCAGGCACTCGGCGCCAAGGGGCTGGTGCAGCCGCTGAAACTATCCTGCTCCGACCATGAAGGCGGTGGCGCGGTGCGCTTCCAGCAGTGGGACGGGCAGAAGTGGAAACTGATCAGCGAC  >  NC_002947/3154719‑3155014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑