Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,177,432 G→T 14.0% L111I (CTC→ATC)  PP_2788 ← MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,177,4320GT14.0% 107.0 / 9.4 43L111I (CTC→ATC) PP_2788MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (20/17);  new base T (3/3);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

CGGGGGGTGGGGCTGTGCCAGGCTAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCGATGATCCTGCAGAATGGCCTG  >  NC_002947/3177284‑3177576
                                                                                                                                                    |                                                                                                                                                 
cGGGGGGTGGGGCTGTGCCAGGCTAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAg                                                                                                                                                   <  2:262114/149‑1 (MQ=255)
    gggTGGGGCTGTGCCAGGCTAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGgctc                                                                                                                                               <  4:49345/149‑1 (MQ=255)
              gtgCCAGGCTAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCCTCAAGGATgctcagat                                                                                                                                           <  6:114709/143‑3 (MQ=255)
              gtgCCAGGCTAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCCTCAAGGATgctcagat                                                                                                                                           >  5:114709/1‑141 (MQ=255)
                gCCAGGCTAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTc                                                                                                                                    >  1:103732/1‑148 (MQ=255)
                  cAGGCTAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAAATGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAAc                                                                                                                                 >  8:235039/1‑149 (MQ=255)
                       tAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCCTCAAGGATgctcaga                                                                                                                                            >  1:58410/1‑132 (MQ=255)
                       tAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCCTCAAGGATgctcaga                                                                                                                                            <  2:58410/133‑2 (MQ=255)
                       tAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGc                                                                                                                            >  8:146884/1‑149 (MQ=255)
                          aTCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCCTCAAGGATgctcagatgc                                                                                                                                         >  3:226476/1‑129 (MQ=255)
                          aTCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCCTCAAGGATgctcagatgc                                                                                                                                         <  4:226476/133‑5 (MQ=255)
                           tCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGt                                                                                                                         >  4:154785/1‑148 (MQ=255)
                                ggggCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCa                                                                                                                   >  5:345300/1‑149 (MQ=255)
                                          cGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGcc                                                                                                                           >  4:310865/1‑131 (MQ=255)
                                          cGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGcc                                                                                                                           <  3:310865/131‑1 (MQ=255)
                                              cTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGcc                                                                                                     >  4:262035/1‑149 (MQ=255)
                                               tGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCTTCATTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGcat                                                                                                    >  6:279443/1‑147 (MQ=255)
                                                 aaaaCGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTgg                                                                                                  <  4:342325/149‑1 (MQ=255)
                                                 aaaaCGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTgg                                                                                                  <  8:349299/149‑1 (MQ=255)
                                                 aaaaCGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTg                                                                                                   >  5:323575/1‑148 (MQ=255)
                                                   aaCGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCCGGCTTTTTCCAGGTCTTCGAACTGGCATTGCATCTGCGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCAAGCACTTGCTGCAAGGCCTGGAt                                                                                                >  5:257893/1‑149 (MQ=255)
                                                         aCCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGccc                                                                                          <  2:195996/149‑1 (MQ=255)
                                                          ccGGGCGCCATGGCCGTTCAGGGGCGGCGGTGCGGTTTCAGCGGGCTTTTTCCAGGGGTTAGAACTGGCATTGCATCTTATCATCCTTTAGGCTCAGCTCAAACTCAACCAATCCGTCATGCACTTGCTGCAAGGCCTGGATCTGcccc                                                                                         <  7:235039/149‑1 (MQ=255)
                                                                    tgtccGTTCAGGGGCGGCGGAGCTGATTCAGCTGGCTTTTTCCAGGTTTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGGCCCATCAATTCCt                                                                               <  3:59640/146‑1 (MQ=255)
                                                                                   ggcggAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGa                                                               <  7:146884/149‑1 (MQ=255)
                                                                                            gaTTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCa                                                      >  7:340935/1‑149 (MQ=255)
                                                                                             aTTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCAt                                                     >  8:111688/1‑149 (MQ=255)
                                                                                                  gCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCTAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCa                                                <  5:83160/149‑1 (MQ=255)
                                                                                                   gggggTTTTTCCATGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTGAACCAAGCCGTCATGGACTTGGTGCTAGGCCTGGATCTGCCCGATAAATTCCTGACTTTTTGTTATCGATGGCCTGCATGGCCa                                                <  1:27591/148‑1 (MQ=255)
                                                                                                             ccAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATTCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAggg                                      <  6:323575/148‑1 (MQ=255)
                                                                                                                             gCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGcc                                                          >  1:26782/1‑112 (MQ=255)
                                                                                                                             gCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGcc                                                          <  2:26782/112‑1 (MQ=255)
                                                                                                                             gCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCga                      <  3:154785/148‑1 (MQ=255)
                                                                                                                               aTTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCgatga                   <  2:103732/149‑1 (MQ=255)
                                                                                                                                ttGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCgatgat                  >  7:102686/1‑149 (MQ=255)
                                                                                                                                   cATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCGATGATcc                <  6:345300/148‑1 (MQ=255)
                                                                                                                                        gAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCGATGATCCTGCAg           >  8:24889/1‑148 (MQ=255)
                                                                                                                                         aGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCGATGATCCTGCa            <  8:102686/146‑1 (MQ=255)
                                                                                                                                           cATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCGATGATCCTGCAGAATg       >  5:278444/1‑149 (MQ=255)
                                                                                                                                           cATCCTTGAGCCTAAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGCCAGCTCCTGCCCGCGATGATACTGCAGAATg       >  2:254031/1‑149 (MQ=255)
                                                                                                                                               cTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCGATGATCCTGCAGAATGGCCt   >  4:340230/1‑149 (MQ=255)
                                                                                                                                                ttGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCAGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCGATGATCCTGCAGAATGGCCTg  >  2:284850/1‑149 (MQ=255)
                                                                                                                                                  gAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTc                                <  4:366648/117‑1 (MQ=255)
                                                                                                                                                  gAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTc                                >  3:366648/1‑117 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGGGGGGTGGGGCTGTGCCAGGCTAAATCGCCGGGGCGCCTGCGTGCTGAAAACGGCACCGGGCGCCATGGCCGTTCAGGGGCGGCGGAGCGGATTCAGCGGGCTTTTTCCAGGTGTTCGAACTGGCATTGCATCTGAGCATCCTTGAGGCTCAGCTCAAACTCAACCAAGCCGTCATGCACTTGCTGCAAGGCCTGGATCTGCCCCATCAATTCCTG‑CTTTTTGTTATCGATGGCCTGCATGGCCAGATCCCAGGGCAGCTCCTGGCCGCGATGATCCTGCAGAATGGCCTG  >  NC_002947/3177284‑3177576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑