Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,208,582	T→C	5.2%	V744A (GTG→GCG)	PP_2812 →	transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,208,582	0	T	C	5.2%	230.2 / 2.2	76	V744A (GTG→GCG)	PP_2812	transporter
Reads supporting (aligned to +/- strand):  ref base T (38/34);  new base C (2/2);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

CCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGCGGGTATTCCCACGT  >  NC_002947/3208435‑3208727                                                                                                                                                      |                                                                                                                                                  ccGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTg                                                                                                                                                  <  1:200488/149‑1 (MQ=255)  cGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTgg                                                                                                                                                 <  5:202218/149‑1 (MQ=255)          ttGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGc                                                                                                                                          >  7:161141/1‑148 (MQ=255)              tgtCGGCTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCt                                                                                                                                     >  4:37382/1‑149 (MQ=255)                  gACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGc                                                                                                                                 >  4:206625/1‑149 (MQ=255)                    cTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTg                                                                                                                                            >  1:370708/1‑136 (MQ=255)                    cTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTg                                                                                                                                            <  2:370708/136‑1 (MQ=255)                          cTTCTACATCTAGAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGAATTGCTCGAGGTGGGCGTTGCGACTATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTtctc                                                                                                                           <  8:310336/147‑1 (MQ=255)                          cTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCg                                                                                                                          >  6:186581/1‑148 (MQ=255)                                catctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCTGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTc                                                                                                                    <  8:161141/148‑1 (MQ=255)                                catctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGc                                                                                                                      <  1:320880/146‑1 (MQ=255)                                catctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGc                                                                                                                      <  2:57129/146‑1 (MQ=255)                                catctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTc                                                                                                                    >  1:196295/1‑148 (MQ=255)                                 atctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCt                                                                                                                     >  8:285908/1‑146 (MQ=255)                                 atctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCt                                                                                                                     <  7:285908/146‑1 (MQ=255)                                 atctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCaa                                                                                                                  <  3:37382/149‑1 (MQ=255)                                  tctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTc                                                                                                                    >  6:235673/1‑146 (MQ=255)                                  tctacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTc                                                                                                                    <  5:235673/146‑1 (MQ=255)                                    tacaACCGGCTGCAACTGCACCTGGCCCTACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGt                                                                                                                >  1:22541/1‑149 (MQ=255)                                    tacaACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGtt                                                                                                               >  2:367912/1‑149 (MQ=255)                                      caACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTc                                                                                                              <  2:22541/148‑1 (MQ=255)                                        aCCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAg                                                                                                           <  5:375841/149‑1 (MQ=255)                                           ggCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGcc                                                                                                        <  5:186581/149‑1 (MQ=255)                                              tGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGa                                                                                                      >  3:361290/1‑148 (MQ=255)                                                 aaCTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATg                                                                                                  >  7:16547/1‑149 (MQ=255)                                                      cACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGc                                                                                               >  5:355037/1‑147 (MQ=255)                                                       aCCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCaa                                                                                             <  2:196295/148‑1 (MQ=255)                                                         cTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGCCGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAgct                                                                                          >  2:371226/1‑149 (MQ=255)                                                           ggCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAgctgc                                                                                        >  3:368691/1‑149 (MQ=255)                                                            gCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAgctgct                                                                                       >  3:298251/1‑149 (MQ=255)                                                             ccc‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGctgctg                                                                                      <  1:335167/149‑1 (MQ=255)                                                              cc‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGCgggtag                                                                                                                                             >  4:252445/1‑90 (MQ=255)                                                              cc‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGCgggtag                                                                                                                                             >  4:247582/1‑90 (MQ=255)                                                              cc‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGCgggtag                                                                                                                                             <  3:252445/93‑4 (MQ=255)                                                              cc‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGCgggtag                                                                                                                                             <  3:247582/93‑4 (MQ=255)                                                                    gCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGTAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCtt                                                                                 >  4:223409/1‑148 (MQ=255)                                                                     ccAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCa                                                                               <  1:367912/149‑1 (MQ=255)                                                                     ccAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCa                                                                               <  3:206625/149‑1 (MQ=255)                                                                       aGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATg                                                                             >  1:6848/1‑149 (MQ=255)                                                                            aTCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTgg                                                                                     <  1:160369/136‑1 (MQ=255)                                                                            aTCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTgg                                                                                     >  2:160369/1‑136 (MQ=255)                                                                            aTCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCAt                                                                        >  8:43416/1‑149 (MQ=255)                                                                             tCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATc                                                                       <  3:51633/149‑1 (MQ=255)                                                                                ccAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGCTCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCGGGGCGGTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCCAGCTGCTGGCATTCATGTTCATCgg                                                                     >  8:171034/1‑147 (MQ=39)                                                                                ccAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGt                                                                     >  2:230497/1‑148 (MQ=255)                                                                                       ggTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATgg                                                             >  3:164151/1‑149 (MQ=255)                                                                                         tCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATAGTCAACATGGTc                                                           <  4:368691/149‑1 (MQ=255)                                                                                            aGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATg                                                        <  8:16547/149‑1 (MQ=255)                                                                                             gCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATgg                                                       >  8:332232/1‑149 (MQ=255)                                                                                              cACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGTGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGc                                                      >  8:290720/1‑149 (MQ=255)                                                                                                 gCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAgc                                                                                           >  6:302879/1‑109 (MQ=255)                                                                                                 gCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCAt                                                   >  4:270811/1‑149 (MQ=255)                                                                                                        tCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATc                                                                       >  7:12392/1‑122 (MQ=255)                                                                                                        tCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATc                                                                       <  8:12392/122‑1 (MQ=255)                                                                                                          gAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGtgct                                          >  2:63428/1‑149 (MQ=255)                                                                                                          gAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGtgct                                          >  1:215385/1‑149 (MQ=255)                                                                                                                ggCGTTGCGACGATCTTCACCGCCATTACCCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCgg                                     >  1:221487/1‑149 (MQ=255)                                                                                                                ggCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGc                                    >  8:172511/1‑149 (MQ=255)                                                                                                                 gCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGcc                                   >  5:61928/1‑149 (MQ=255)                                                                                                                        gacgaTCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCgg                            <  7:43416/149‑1 (MQ=255)                                                                                                                        gacgaTCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCgg                            >  3:14780/1‑149 (MQ=255)                                                                                                                         acgaTCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGt                           <  6:355037/149‑1 (MQ=255)                                                                                                                          cgaTCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTc                          >  6:318037/1‑149 (MQ=255)                                                                                                                             tCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTctg                        <  4:164151/148‑1 (MQ=255)                                                                                                                                 cACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTctggctg                    <  2:221487/148‑1 (MQ=255)                                                                                                                                 cACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTctggctg                    <  3:270811/148‑1 (MQ=255)                                                                                                                                 cACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTctggc                      <  1:230497/146‑1 (MQ=255)                                                                                                                                  aCCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCACGCTGCGGGCCTTAATGTTCAACGTCAACATGGTCATGGACATGACCGTGCTGACGGCCTTCGCTggctggctc                    >  6:10389/1‑146 (MQ=38)                                                                                                                                  aCCGCAATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTGCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCACCATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTctggctg                    <  5:10389/147‑1 (MQ=255)                                                                                                                                    cGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGc                >  4:358646/1‑149 (MQ=255)                                                                                                                                        aTTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGCGGGt            <  7:172511/149‑1 (MQ=255)                                                                                                                                             cccTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGCGGGTATTc        <  4:361290/149‑1 (MQ=255)                                                                                                                                             cccTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGCGGGTATTc        <  4:298251/149‑1 (MQ=255)                                                                                                                                               cTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGCGGGTATTccc      <  4:14780/149‑1 (MQ=255)                                                                                                                                                  gCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGCGGGTATTCCCAc    <  1:63428/148‑1 (MQ=255)                                                                                                                                                    ggTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGCGGGTATTCCCACGt  >  7:82865/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCC‑ACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTA‑CCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTCAACATGGTCATGGCCATGACCGTGCTGCCGGCCTTCGCGGTCTGGCTGGAGCGGGTATTCCCACGT  >  NC_002947/3208435‑3208727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑