Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,211,101 A→G 5.4% E46E (GAA→GAG)  PP_2815 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,211,1010AG 5.4% 224.6 / 2.2 74E46E (GAA→GAG) PP_2815hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/37);  new base G (2/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CACCGCCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGACATGGTGGACAC  >  NC_002947/3210956‑3211244
                                                                                                                                                 |                                                                                                                                               
cACCGCCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGAt                                                                                                                                              <  7:3356/149‑1 (MQ=255)
    gCCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCa                                                                                                                                          <  6:340339/149‑1 (MQ=255)
    gCCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCa                                                                                                                                          <  7:48756/149‑1 (MQ=255)
      ccATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGTTTTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAgg                                                                                                                                        <  1:304450/149‑1 (MQ=255)
          gACCAGCACGCCGTCGCCCTGGCCTCGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAGGATGCCAGGTTGc                                                                                                                                    >  4:203247/1‑149 (MQ=255)
          gACCAGCACGCCGTCGCCCTGGCCTCGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAGGATGCCAGGTTGc                                                                                                                                    >  4:208086/1‑149 (MQ=255)
           aCCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATg                                                                                                                                             <  6:239758/139‑1 (MQ=255)
           aCCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATg                                                                                                                                             >  5:239758/1‑139 (MQ=255)
              aGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTgga                                                                                                                                <  6:327691/149‑1 (MQ=255)
                   gCCGTCGCCCTGGGCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGAcc                                                                                                                           >  5:148340/1‑149 (MQ=255)
                   gCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGAcc                                                                                                                           >  4:253237/1‑149 (MQ=255)
                     cGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCGTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCg                                                                                                                         >  1:303317/1‑149 (MQ=255)
                      gTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCgg                                                                                                                        <  3:220983/149‑1 (MQ=255)
                           ccTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATc                                                                                                                   >  4:174635/1‑149 (MQ=255)
                               gCCTCGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAGGATGCCAGGTTGCTGGAGGACCCGGTCATCCTc                                                                                                                <  3:203247/148‑1 (MQ=255)
                               gCCTCGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAGGATGCCAGGTTGCTGGAGGACCCGGTCATCCTc                                                                                                                <  3:208086/148‑1 (MQ=255)
                                 cACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTCCCCGCCCAGTCGGTCGAGCGCCTGAACCACTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGACATCCTcgcg                                                                                                             >  5:370309/1‑149 (MQ=255)
                                  aCGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGaa                                                                                                                                                 <  8:167978/112‑1 (MQ=255)
                                  aCGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGaa                                                                                                                                                 >  7:167978/1‑112 (MQ=255)
                                      cccTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATACTCGCGGCGTg                                                                                                        >  5:205504/1‑149 (MQ=255)
                                      cccTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTCCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCAt                                                                                                                    <  7:247757/137‑1 (MQ=255)
                                      cccTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTCCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCAt                                                                                                                    >  8:247757/1‑137 (MQ=255)
                                            gAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCgcggc                                                                                                           <  6:209974/140‑1 (MQ=255)
                                            gAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCgcggc                                                                                                           >  5:209974/1‑140 (MQ=255)
                                                       cgAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCggg                                                                                       >  1:279494/1‑149 (MQ=255)
                                                        gAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGCGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGc                                                                                      <  1:347539/149‑1 (MQ=255)
                                                           cTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGACGGCGCGCCAACCGGGCaga                                                                                   <  6:205504/149‑1 (MQ=255)
                                                           cTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCACCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCaga                                                                                   <  8:333905/149‑1 (MQ=255)
                                                               tGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCACCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGtt                                                                                <  4:136050/148‑1 (MQ=255)
                                                                  gcAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTtgctg                                                                            >  1:166640/1‑149 (MQ=255)
                                                                  gcAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTtgctg                                                                            >  4:114111/1‑149 (MQ=255)
                                                                   cAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTtgctgc                                                                           <  8:104898/149‑1 (MQ=255)
                                                                        gcgcCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTgg                                                                      >  8:191427/1‑149 (MQ=255)
                                                                          gcCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGc                                                                    >  7:324903/1‑149 (MQ=255)
                                                                             gAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCaa                                                                 <  8:266158/149‑1 (MQ=255)
                                                                              aaCTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAAt                                                                >  3:54345/1‑149 (MQ=255)
                                                                              aaCTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAAt                                                                >  3:153962/1‑149 (MQ=255)
                                                                                   gCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGt                                                                                                         >  2:59738/1‑103 (MQ=255)
                                                                                   gCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGt                                                                                                         <  1:59738/103‑1 (MQ=255)
                                                                                     tCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACAGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTCGGCCAATGCCAAcc                                                         >  7:289914/1‑149 (MQ=255)
                                                                                        ggCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGc                                                      <  3:174635/149‑1 (MQ=255)
                                                                                         gcgcTGCCAGCCGAGTGGGTCGAGCGGCTGAAGCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCa                                                     <  8:289914/149‑1 (MQ=255)
                                                                                         gcgcTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCa                                                     <  2:303317/149‑1 (MQ=255)
                                                                                              gCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGTGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGcc                                                >  2:9342/1‑149 (MQ=255)
                                                                                               ccAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGccc                                               >  3:61969/1‑149 (MQ=255)
                                                                                               ccAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGccc                                               >  6:309373/1‑149 (MQ=255)
                                                                                                cAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCt                                              <  2:279494/149‑1 (MQ=255)
                                                                                                cAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCt                                              >  4:2527/1‑149 (MQ=255)
                                                                                                   ccGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTggg                                           <  2:166640/149‑1 (MQ=255)
                                                                                                     gAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTggg                                           >  2:142476/1‑147 (MQ=255)
                                                                                                        tGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGcc                                      <  4:54345/149‑1 (MQ=255)
                                                                                                             cGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTgg                                 <  7:191427/149‑1 (MQ=255)
                                                                                                              gAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGa                                <  3:253237/149‑1 (MQ=255)
                                                                                                                 cgcCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTg                                  >  4:329227/1‑144 (MQ=255)
                                                                                                                 cgcCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTg                                  <  3:329227/144‑1 (MQ=255)
                                                                                                                   ccTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTtgct                                                                             <  5:62295/99‑1 (MQ=255)
                                                                                                                   ccTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTtgct                                                                             >  6:62295/1‑99 (MQ=255)
                                                                                                                       aaCCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGTGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTAt                         <  1:9342/147‑1 (MQ=255)
                                                                                                                       aaCCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTAt                         <  8:324903/147‑1 (MQ=255)
                                                                                                                       aaCCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTAt                         <  1:142476/147‑1 (MQ=255)
                                                                                                                       aaCCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTAt                         <  4:188615/147‑1 (MQ=255)
                                                                                                                             tCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGa                                         >  6:197207/1‑125 (MQ=255)
                                                                                                                             tCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGa                                         <  5:197207/125‑1 (MQ=255)
                                                                                                                               aCTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGGCAAGCTGCAGCGCCGTTGGGAGCCGGTGGAGCCCTATGTGTgtgctg               <  6:148340/149‑1 (MQ=255)
                                                                                                                               aCTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTctgct                >  7:232737/1‑148 (MQ=255)
                                                                                                                                 tGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTctgc                 >  4:368547/1‑145 (MQ=255)
                                                                                                                                 tGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTctgc                 <  3:368547/145‑1 (MQ=255)
                                                                                                                                  gTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGACa            >  2:335946/1‑149 (MQ=255)
                                                                                                                                   ttCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGAc             <  3:2527/147‑1 (MQ=255)
                                                                                                                                   ttCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGACa            <  4:61969/148‑1 (MQ=255)
                                                                                                                                      tctTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGACAtggt        >  2:351751/1‑149 (MQ=255)
                                                                                                                                        ttCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGACAtggtgg      >  8:236491/1‑149 (MQ=255)
                                                                                                                                        ttCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGACAtggtg       >  6:148647/1‑148 (MQ=255)
                                                                                                                                           gCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTAt                         <  3:292632/127‑1 (MQ=255)
                                                                                                                                           gCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTAt                         >  4:292632/1‑127 (MQ=255)
                                                                                                                                            cccGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGACATGGTGGacac  <  5:309373/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CACCGCCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGCCAGCCGAGTGGGTCGAGCGCCTGAACCAGTCACTGTTCTCTTCGCCCGAAGATGCCAGGTTGCTGGAGGACCCGGTCATCCTCGCGGCGTGCCGGCGCGCCAACCGGGCAGAGTTGCTGCACTGGGCCAATGCCAACCTGCAGCGCCCTGGGGAGCCGGTGGAGCCCTATGTGTCTGCTGACATGGTGGACAC  >  NC_002947/3210956‑3211244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑