Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,261,387 T→A 6.4% intergenic (‑129/+169) PP_2858 ← / ← PP_2859 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,261,3870TA 6.4% 192.7 / 3.0 62intergenic (‑129/+169)PP_2858/PP_2859hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base A (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

TCCTCACGACGGCAACTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACGGGGGGAAGCCCCGCAATTTCGGGGGCTTTG  >  NC_002947/3261252‑3261532
                                                                                                                                       |                                                                                                                                                 
tCCTCACGACGGCAACTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCt                                                                                                                                      >  2:14919/1‑149 (MQ=255)
  cTCACGACGGCAACTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCtt                                                                                                                                     <  1:14919/148‑1 (MQ=255)
   tCACGACGGCAACTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCAAGCTGTTTCAAGcct                                                                                                                                     >  5:154158/1‑145 (MQ=255)
   tCACGACGGCAACTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCAAGCTGTTTCAAGcct                                                                                                                                     >  5:164476/1‑145 (MQ=255)
     acgacgGCAACTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGaaa                                                                                                                                 <  4:84800/149‑1 (MQ=255)
       gacgGCAACTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGaaa                                                                                                                                 >  6:172302/1‑147 (MQ=255)
              aCTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCAAGCTGTTTCAAGCCTGAGACAGCATGcg                                                                                                                        <  6:154158/149‑2 (MQ=255)
              aCTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCAAGCTGTTTCAAGCCTGAGACAGCATGcg                                                                                                                        <  6:164476/149‑2 (MQ=255)
              aCTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCACTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGcc                                                                                                                        <  1:132578/149‑1 (MQ=255)
                 ccTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGATGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTg                                                                                                                     >  1:8684/1‑149 (MQ=255)
                     cttTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTgcgc                                                                                                                  <  1:263135/148‑1 (MQ=255)
                       ttGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTgcgc                                                                                                                  >  3:345858/1‑146 (MQ=255)
                       ttGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTgcgc                                                                                                                  <  4:345858/146‑1 (MQ=255)
                         gAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTg                                                                                                              <  5:234596/148‑1 (MQ=255)
                          aGCGCTGGCCCTGTTGATCGATGCGAACGATAGAGCAGAAGTTGTGCCGGTTTTTAGAATACGTTTCATATCAGATAGTTAGGAAAAGAGGTTGATATAATGCGGCGCATGCTGtct                                                                                                                                            <  2:356001/117‑1 (MQ=255)
                          aGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGGGTGCCGCTTTTTAGAATACGTTTCATATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGcc                                                                                                            <  7:328405/149‑1 (MQ=255)
                          aGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAACGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGtct                                                                                                                                            >  1:356001/1‑117 (MQ=255)
                            cgcTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCgg                                                                                                          <  8:225038/149‑1 (MQ=255)
                              cTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGc                                                                                                         >  4:278463/1‑148 (MQ=255)
                               tGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCgg                                                                                                          >  3:11791/1‑146 (MQ=255)
                               tGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCgg                                                                                                          <  4:11791/146‑1 (MQ=255)
                                   ccTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTAAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGt                                                                                                    >  5:60836/1‑148 (MQ=255)
                                       ttCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAg                                                                                                 <  5:149368/147‑1 (MQ=255)
                                          aGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATAGGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCtgtg                                                                                            >  8:226870/1‑149 (MQ=255)
                                          aGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTAAGCtgtg                                                                                            >  5:138293/1‑149 (MQ=255)
                                                gCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCtt                                                                                       >  3:20203/1‑148 (MQ=255)
                                                  gAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGAt                                                                                    >  7:52157/1‑149 (MQ=255)
                                                  gAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGAt                                                                                    >  5:23933/1‑149 (MQ=255)
                                                       aTAGAGCAGAAGGTGTGCCGCTTCTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGaa                                                                               <  2:240290/149‑1 (MQ=255)
                                                          gagCAGAAGGTGTGCCGCTTTTTAGAATATGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAggg                                                                            <  7:226870/149‑1 (MQ=255)
                                                          gagCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAggg                                                                            >  7:213866/1‑149 (MQ=255)
                                                           agCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGt                                                                           >  8:91873/1‑149 (MQ=255)
                                                            gCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGtt                                                                          >  1:317205/1‑149 (MQ=255)
                                                              aGATGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGtttt                                                                        <  2:8684/149‑1 (MQ=255)
                                                               gAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGttttt                                                                       <  8:293358/149‑1 (MQ=255)
                                                               gAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGttttt                                                                       <  4:345943/149‑1 (MQ=255)
                                                               gAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGttttt                                                                       <  4:134355/149‑1 (MQ=255)
                                                                  ggTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGcc                                                                                         >  5:134519/1‑128 (MQ=255)
                                                                  ggTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGcc                                                                                         <  6:134519/128‑1 (MQ=255)
                                                                        ccGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGAGGGGAAGGGTTTTTGCAAgtgtg                                                              >  6:347112/1‑149 (MQ=255)
                                                                          gCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGGTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGtt                                                            <  3:95810/149‑1 (MQ=255)
                                                                              tttAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCa                                                         <  3:21853/148‑1 (MQ=255)
                                                                               ttAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCAc                                                        <  4:20203/148‑1 (MQ=255)
                                                                                  gAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACggg                                                     <  8:52157/148‑1 (MQ=255)
                                                                                  gAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGc                                                    >  3:100947/1‑149 (MQ=255)
                                                                                     tACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTg                                                 <  3:278463/149‑1 (MQ=255)
                                                                                        gTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACgg                                                      >  8:229010/1‑141 (MQ=255)
                                                                                        gTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACgg                                                      <  7:229010/141‑1 (MQ=255)
                                                                                                   gatAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGTCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAg                                    >  7:268841/1‑148 (MQ=255)
                                                                                                   gatAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAgtgtgt                                                             >  4:158146/1‑123 (MQ=255)
                                                                                                   gatAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAgtgtgt                                                             <  3:158146/123‑1 (MQ=255)
                                                                                                           ggAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACggggg                            <  2:317205/148‑1 (MQ=255)
                                                                                                           ggAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACggggg                            >  6:117953/1‑148 (MQ=255)
                                                                                                           ggAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACggggg                            <  6:60836/148‑1 (MQ=255)
                                                                                                            gAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACgggggg                           >  3:337151/1‑148 (MQ=255)
                                                                                                                      tGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACGGGGGGAAGCCCCGCaa                >  7:215362/1‑149 (MQ=255)
                                                                                                                       gATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACGGGGGGAAGCCCCGCAAt               >  3:259722/1‑149 (MQ=255)
                                                                                                                       gATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACGGGGGGAAGCCCCGCAAt               >  6:194871/1‑149 (MQ=255)
                                                                                                                        aTAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGttttt                                                                       <  7:261336/92‑1 (MQ=255)
                                                                                                                        aTAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGttttt                                                                       >  8:261336/1‑92 (MQ=255)
                                                                                                                                   cgcATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACGGGGGGAAGCCCCGCAATTTCGGGGGCttt   <  8:268841/149‑1 (MQ=255)
                                                                                                                                    gcATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACGGGGGGAAGCCCCGCAATTTCGGGGGCTTTg  <  5:347112/149‑1 (MQ=255)
                                                                                                                                       tGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGACAAAGTCAGc                                                                                                >  8:206912/1‑52 (MQ=255)
                                                                                                                                       |                                                                                                                                                 
TCCTCACGACGGCAACTCCTTCTTTGAGCGCTGGCCCTGTTCAGCGATGCGAACGATAGAGCAGAAGGTGTGCCGCTTTTTAGAATACGTTTCAGATCAGATAGTTAGGAAAAGAGGTTGATAAAATGCGGCGCATGCTGTCTCAGGCTTGAAACAGCTTGCCCTGCGCGTTGCCGGCAAAGTCAGCTGTGGCCTTGATGGGAAGGGTTTTTGCAAGTGTGTTTCACGGGCTTGACACTGCCCCCAGGACGGGGGGAAGCCCCGCAATTTCGGGGGCTTTG  >  NC_002947/3261252‑3261532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑