Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,300,857 A→T 6.6% I17N (ATT→AAT)  lpxH ← UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,300,8570AT 6.6% 182.4 / 3.2 61I17N (ATT→AAT) lpxHUDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base A (28/29);  new base T (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

CTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGA  >  NC_002947/3300710‑3301000
                                                                                                                                                   |                                                                                                                                               
cTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGaaa                                                                                                                                                 >  8:190373/1‑148 (MQ=255)
 tCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAata                                                                                                                                               >  5:127460/1‑149 (MQ=255)
      cGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                          >  4:21811/1‑149 (MQ=255)
           cATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGtt                                                                                                                                     <  2:97302/149‑1 (MQ=255)
            aTGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                                    >  6:258097/1‑149 (MQ=255)
             tGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttc                                                                                                                                   <  3:92273/149‑3 (MQ=255)
              ggCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttct                                                                                                                                   >  4:61145/1‑148 (MQ=255)
                  tGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                          <  1:265318/137‑1 (MQ=255)
                  tGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                          >  2:265318/1‑137 (MQ=255)
                                 tgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatca                                                                                                                 >  3:364992/1‑147 (MQ=255)
                                   gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcagaga                                                                                                             <  4:181964/149‑1 (MQ=255)
                                   gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatca                                                                                                                 >  7:2299/1‑145 (MQ=255)
                                   gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttc                                                                                                                                   >  6:194094/1‑125 (MQ=255)
                                   gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttc                                                                                                                                   <  5:194094/127‑3 (MQ=255)
                                         cGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATcagca                                                                                                       <  5:258097/149‑1 (MQ=255)
                                          gTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGc                                                                                                                             >  1:92763/1‑126 (MQ=255)
                                          gTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGc                                                                                                                             <  2:92763/126‑1 (MQ=255)
                                                 cATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACt                                                                                               <  2:50702/149‑1 (MQ=255)
                                                     gtcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACTCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCaa                                                                                            <  3:21811/148‑1 (MQ=255)
                                                      tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                           >  5:101508/1‑148 (MQ=255)
                                                      tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                           >  1:276817/1‑148 (MQ=255)
                                                      tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATg                                                                                          <  4:364992/149‑1 (MQ=255)
                                                      tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATg                                                                                          <  3:61145/149‑1 (MQ=255)
                                                         ccAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGAtc                                                                                       <  6:101508/149‑1 (MQ=255)
                                                           aaTCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGAtctc                                                                                     <  6:153417/149‑1 (MQ=255)
                                                             tCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTAgg                                                                                   >  5:176737/1‑149 (MQ=255)
                                                                aCGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCtt                                                                                >  4:154672/1‑149 (MQ=255)
                                                                  gCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtc                                                                              <  3:221989/149‑1 (MQ=255)
                                                                       gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTAGGCTTTCTCgat                                                                         >  1:206472/1‑149 (MQ=255)
                                                                       gaagaaGTAACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCTAGCAGATAAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTTCAGATAAGAGATCAGCAGAATCACTCAATGATCTCTGCTTTCTCgat                                                                         >  1:200696/1‑149 (MQ=255)
                                                                           aagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgatgat                                                                      >  3:361520/1‑148 (MQ=255)
                                                                           aagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATc                                                                     <  6:127460/149‑1 (MQ=255)
                                                                             gTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAc                                                                   >  3:275635/1‑149 (MQ=255)
                                                                                aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcaga                                                                                                               >  1:249589/1‑102 (MQ=255)
                                                                                aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcaga                                                                                                               <  2:249589/102‑1 (MQ=255)
                                                                                aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtc                                                                >  1:235492/1‑149 (MQ=255)
                                                                                aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtc                                                                >  1:65240/1‑149 (MQ=255)
                                                                                   aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACgtcgtc                                                             >  4:120851/1‑149 (MQ=255)
                                                                                   aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACgtcgtc                                                             >  5:78815/1‑149 (MQ=255)
                                                                                      gATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCtt                                                          >  1:161879/1‑149 (MQ=255)
                                                                                        tGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCg                                                        <  8:2299/149‑1 (MQ=255)
                                                                                            cAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTa                                                     <  4:361520/148‑1 (MQ=255)
                                                                                                  cTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtg                                              <  6:78815/149‑1 (MQ=255)
                                                                                                     ggCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtggt                                            <  3:154672/148‑1 (MQ=255)
                                                                                                               gcgcGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTg                                 <  7:190373/149‑1 (MQ=255)
                                                                                                                 gcgGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGa                               <  5:145389/149‑1 (MQ=255)
                                                                                                                        tCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTg                        <  4:275635/149‑1 (MQ=255)
                                                                                                                         cGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTgg                       >  4:89892/1‑149 (MQ=255)
                                                                                                                               gATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACtt                 <  2:161879/149‑1 (MQ=255)
                                                                                                                                aTCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttt                >  7:75484/1‑149 (MQ=255)
                                                                                                                                aTCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttt                >  6:119875/1‑149 (MQ=255)
                                                                                                                                 tCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACtttt               <  2:235492/149‑1 (MQ=255)
                                                                                                                                    aGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTc                                                 >  5:259291/1‑112 (MQ=255)
                                                                                                                                    aGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTc                                                 <  6:259291/112‑1 (MQ=255)
                                                                                                                                     gAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGa           >  3:126187/1‑149 (MQ=255)
                                                                                                                                     agaaCGCCCGGATATTTCCCGGGCGTTTTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGa           >  8:116283/3‑149 (MQ=255)
                                                                                                                                      aaaCGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt          <  2:65240/149‑1 (MQ=255)
                                                                                                                                        aCGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATgg        <  2:276817/149‑1 (MQ=255)
                                                                                                                                          gCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCtt                                                          >  4:71031/1‑97 (MQ=255)
                                                                                                                                          gCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCtt                                                          <  3:71031/97‑1 (MQ=255)
                                                                                                                                          gCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt              <  4:324054/141‑1 (MQ=255)
                                                                                                                                          gCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt              >  3:324054/1‑141 (MQ=255)
                                                                                                                                              gggAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGa  <  3:147640/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGA  >  NC_002947/3300710‑3301000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑