Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,300,860 T→C 6.5% D16G (GAT→GGT) ‡ lpxH ← UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,300,8600TC 6.5% 178.7 / 2.4 61D16G (GAT→GGT) ‡lpxHUDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base C (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGACCACTTCA  >  NC_002947/3300716‑3301008
                                                                                                                                                |                                                                                                                                                    
cGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                                  >  4:21811/1‑149 (MQ=255)
     cATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGtt                                                                                                                                             <  2:97302/149‑1 (MQ=255)
      aTGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                                            >  6:258097/1‑149 (MQ=255)
       tGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttc                                                                                                                                           <  3:92273/149‑3 (MQ=255)
        ggCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttct                                                                                                                                           >  4:61145/1‑148 (MQ=255)
            tGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                                  <  1:265318/137‑1 (MQ=255)
            tGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                                  >  2:265318/1‑137 (MQ=255)
                           tgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatca                                                                                                                         >  3:364992/1‑147 (MQ=255)
                             gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcagaga                                                                                                                     <  4:181964/149‑1 (MQ=255)
                             gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatca                                                                                                                         >  7:2299/1‑145 (MQ=255)
                             gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttc                                                                                                                                           >  6:194094/1‑125 (MQ=255)
                             gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttc                                                                                                                                           <  5:194094/127‑3 (MQ=255)
                                   cGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATcagca                                                                                                               <  5:258097/149‑1 (MQ=255)
                                    gTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGc                                                                                                                                     >  1:92763/1‑126 (MQ=255)
                                    gTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGc                                                                                                                                     <  2:92763/126‑1 (MQ=255)
                                           cATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACt                                                                                                       <  2:50702/149‑1 (MQ=255)
                                               gtcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACTCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCaa                                                                                                    <  3:21811/148‑1 (MQ=255)
                                                tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                                   >  5:101508/1‑148 (MQ=255)
                                                tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                                   >  1:276817/1‑148 (MQ=255)
                                                tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATg                                                                                                  <  4:364992/149‑1 (MQ=255)
                                                tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATg                                                                                                  <  3:61145/149‑1 (MQ=255)
                                                   ccAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGAtc                                                                                               <  6:101508/149‑1 (MQ=255)
                                                     aaTCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGAtctc                                                                                             <  6:153417/149‑1 (MQ=255)
                                                       tCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTAgg                                                                                           >  5:176737/1‑149 (MQ=255)
                                                          aCGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCtt                                                                                        >  4:154672/1‑149 (MQ=255)
                                                            gCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtc                                                                                      <  3:221989/149‑1 (MQ=255)
                                                                 gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTAGGCTTTCTCgat                                                                                 >  1:206472/1‑149 (MQ=255)
                                                                 gaagaaGTAACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCTAGCAGATAAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTTCAGATAAGAGATCAGCAGAATCACTCAATGATCTCTGCTTTCTCgat                                                                                 >  1:200696/1‑149 (MQ=255)
                                                                     aagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgatgat                                                                              >  3:361520/1‑148 (MQ=255)
                                                                     aagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATc                                                                             <  6:127460/149‑1 (MQ=255)
                                                                       gTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAc                                                                           >  3:275635/1‑149 (MQ=255)
                                                                          aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcaga                                                                                                                       <  2:249589/102‑1 (MQ=255)
                                                                          aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcaga                                                                                                                       >  1:249589/1‑102 (MQ=255)
                                                                          aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtc                                                                        >  1:235492/1‑149 (MQ=255)
                                                                          aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtc                                                                        >  1:65240/1‑149 (MQ=255)
                                                                             aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACgtcgtc                                                                     >  4:120851/1‑149 (MQ=255)
                                                                             aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACgtcgtc                                                                     >  5:78815/1‑149 (MQ=255)
                                                                                gATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCtt                                                                  >  1:161879/1‑149 (MQ=255)
                                                                                  tGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCg                                                                <  8:2299/149‑1 (MQ=255)
                                                                                      cAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTa                                                             <  4:361520/148‑1 (MQ=255)
                                                                                            cTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtg                                                      <  6:78815/149‑1 (MQ=255)
                                                                                               ggCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtggt                                                    <  3:154672/148‑1 (MQ=255)
                                                                                                         gcgcGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTg                                         <  7:190373/149‑1 (MQ=255)
                                                                                                           gcgGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGa                                       <  5:145389/149‑1 (MQ=255)
                                                                                                                  tCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTg                                <  4:275635/149‑1 (MQ=255)
                                                                                                                   cGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTgg                               >  4:89892/1‑149 (MQ=255)
                                                                                                                         gATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACtt                         <  2:161879/149‑1 (MQ=255)
                                                                                                                          aTCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttt                        >  7:75484/1‑149 (MQ=255)
                                                                                                                          aTCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttt                        >  6:119875/1‑149 (MQ=255)
                                                                                                                           tCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACtttt                       <  2:235492/149‑1 (MQ=255)
                                                                                                                              aGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTc                                                         >  5:259291/1‑112 (MQ=255)
                                                                                                                              aGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTc                                                         <  6:259291/112‑1 (MQ=255)
                                                                                                                               gAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGa                   >  3:126187/1‑149 (MQ=255)
                                                                                                                               agaaCGCCCGGATATTTCCCGGGCGTTTTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGa                   >  8:116283/3‑149 (MQ=255)
                                                                                                                                aaaCGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt                  <  2:65240/149‑1 (MQ=255)
                                                                                                                                  aCGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATgg                <  2:276817/149‑1 (MQ=255)
                                                                                                                                    gCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCtt                                                                  >  4:71031/1‑97 (MQ=255)
                                                                                                                                    gCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCtt                                                                  <  3:71031/97‑1 (MQ=255)
                                                                                                                                    gCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt                      >  3:324054/1‑141 (MQ=255)
                                                                                                                                    gCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt                      <  4:324054/141‑1 (MQ=255)
                                                                                                                                        gggAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGa          <  3:147640/149‑1 (MQ=255)
                                                                                                                                                tCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGACCACTTCa  >  4:355541/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGACCACTTCA  >  NC_002947/3300716‑3301008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑