Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,301,892 G→A 8.8% D81N (GAT→AAT)  glnS → glutamine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,301,8920GA 8.8% 192.7 / 4.4 67D81N (GAT→AAT) glnSglutamine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base A (4/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTGGACTCGGGCAAGCACAGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGATCAAGCG  >  NC_002947/3301746‑3302040
                                                                                                                                                  |                                                                                                                                                    
aCCTGGACTCGGGCAAGCACAGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGAt                                                                                                                                                    <  3:297636/149‑1 (MQ=255)
       cTCGGGCAAGCACAGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAAcc                                                                                                                                             >  1:8984/1‑149 (MQ=255)
          gggCAAGCACAGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACACTACCAACCCgg                                                                                                                                          >  4:335070/1‑149 (MQ=255)
          gggCAAGCACAGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACAATACCAACCCgg                                                                                                                                          >  4:340560/1‑149 (MQ=255)
            gCAAGCACAGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGATACGACGATACCCACCCGGc                                                                                                                                         >  6:8405/1‑148 (MQ=255)
               aGCACAGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAg                                                                                                                                     <  3:182753/149‑1 (MQ=255)
                  acaGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACAATACCAACCCGGCCAAgg                                                                                                                                    >  2:333736/1‑147 (MQ=255)
                  acaGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACAATACCAACCCGGCCAAGGa                                                                                                                                   >  2:329358/1‑148 (MQ=255)
                     gcagcaTCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGAc                                                                                                                               >  1:176669/1‑149 (MQ=255)
                     gcagcaTCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGAc                                                                                                                               <  2:169896/149‑1 (MQ=255)
                       agcaTCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAAc                                                                                                                                              <  4:93705/132‑1 (MQ=255)
                       agcaTCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAAc                                                                                                                                              >  3:93705/1‑132 (MQ=255)
                        gcaTCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAg                                                                                                                            <  4:83470/149‑1 (MQ=255)
                        gcaTCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAg                                                                                                                            <  4:357996/149‑1 (MQ=255)
                          aTCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGa                                                                                                                          <  3:367303/149‑1 (MQ=255)
                             gTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGt                                                                                                                        <  2:176669/148‑1 (MQ=255)
                              tCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACAATACCAACCCGGCCAAGGAAGACCAGGAGTAc                                                                                                                      <  1:329358/149‑1 (MQ=255)
                              tCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACAATACCAACCCGGCCAAGGAAGACCAGGAGTAc                                                                                                                      <  1:333736/149‑1 (MQ=255)
                                 cccGTTTCTCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAg                                                                                                                                     <  3:163041/131‑1 (MQ=255)
                                 cccGTTTCTCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAg                                                                                                                                     >  4:163041/1‑131 (MQ=255)
                                        cccGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGt                                                                                                                        <  3:185916/137‑1 (MQ=255)
                                        cccGACGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGt                                                                                                                        >  4:185916/1‑137 (MQ=255)
                                          cgccgGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCg                                                                                                                                           <  5:373691/116‑1 (MQ=255)
                                          cgccgGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCg                                                                                                                                           >  6:373691/1‑116 (MQ=255)
                                           gccgGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACAAACCCGGACAAGGAAGACCAGGAGTACATAGACGCCAta                                                                                                          >  4:10479/1‑147 (MQ=255)
                                                   ccAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGTCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGAt                                                                                                 <  4:134043/149‑1 (MQ=255)
                                                   ccAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGAt                                                                                                 <  7:23936/149‑1 (MQ=255)
                                                    cAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGGCCAGGAGTACATCGACGCCATCCAGAGCGATg                                                                                                >  1:31067/1‑149 (MQ=255)
                                                             ccTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGc                                                                                       <  5:8405/149‑1 (MQ=255)
                                                              cTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCt                                                                                      >  4:4778/1‑149 (MQ=255)
                                                              cTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCt                                                                                      >  1:84177/1‑149 (MQ=255)
                                                              cTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCt                                                                                      >  3:315946/1‑149 (MQ=255)
                                                                gCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTgg                                                                                    >  7:355530/1‑149 (MQ=255)
                                                                 cacaTCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTgg                                                                                    <  3:10479/148‑1 (MQ=255)
                                                                        gCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAg                                                                            >  7:189863/1‑149 (MQ=255)
                                                                         ccACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGt                                                                           <  2:84177/149‑1 (MQ=255)
                                                                            cGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTggg                                                                        <  8:355530/149‑1 (MQ=255)
                                                                               cAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTgggg                                                                       <  5:142659/147‑1 (MQ=255)
                                                                               cAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTgggg                                                                       >  6:142659/1‑147 (MQ=255)
                                                                                 aGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGCGCTTCGAGTGGggcggc                                                                   >  6:228319/1‑149 (MQ=255)
                                                                                     gATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGc                                                                                                              >  4:112841/1‑102 (MQ=255)
                                                                                     gATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGc                                                                                                              <  3:112841/102‑1 (MQ=255)
                                                                                     gATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTgg                                                                                    <  8:159744/128‑1 (MQ=255)
                                                                                     gATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTgg                                                                                    >  7:159744/1‑128 (MQ=255)
                                                                                             tCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTAc                                                       >  3:117541/1‑149 (MQ=255)
                                                                                                 cTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGt                                                              >  7:62064/1‑138 (MQ=255)
                                                                                                 cTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGt                                                              <  8:62064/138‑1 (MQ=255)
                                                                                                  ttCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTc                                                  >  3:44219/1‑149 (MQ=255)
                                                                                                         tGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGactac                                           >  5:88279/1‑149 (MQ=255)
                                                                                                             cAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGggcgg                                                                    >  4:40785/1‑120 (MQ=255)
                                                                                                             cAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGggcgg                                                                    <  3:40785/120‑1 (MQ=255)
                                                                                                             cAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTc                                        >  5:4958/1‑148 (MQ=255)
                                                                                                              aaGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGa                                      >  7:240913/1‑149 (MQ=255)
                                                                                                              aaGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGa                                      <  6:88279/149‑1 (MQ=255)
                                                                                                                ggAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGAc                                     >  6:370113/1‑148 (MQ=255)
                                                                                                                 gAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCa                                   <  5:370113/149‑1 (MQ=255)
                                                                                                                   aTTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTc                                        <  8:133531/142‑1 (MQ=255)
                                                                                                                   aTTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTc                                        >  7:133531/1‑142 (MQ=255)
                                                                                                                   aTTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGc                                 <  4:117541/149‑1 (MQ=255)
                                                                                                                           gcgTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGAc                         >  1:109700/1‑149 (MQ=255)
                                                                                                                              tCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTgg                      <  6:149793/149‑1 (MQ=255)
                                                                                                                                    aCCTGCGTTTCGACGATACCAACGCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTc                <  5:228319/149‑1 (MQ=255)
                                                                                                                                      cTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGa              <  4:44219/149‑1 (MQ=255)
                                                                                                                                         cGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGc            <  4:315946/148‑1 (MQ=255)
                                                                                                                                           tttCGACGATACCAACCCGGCCAAGGAAGGCCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGa         <  2:31067/149‑1 (MQ=255)
                                                                                                                                           tttCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGa         >  3:205816/1‑149 (MQ=255)
                                                                                                                                           atctgacgaTACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGa         <  3:236119/145‑1 (MQ=255)
                                                                                                                                             tCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGAt        <  8:240913/148‑1 (MQ=255)
                                                                                                                                               gacgaTACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGATc       >  8:285713/1‑147 (MQ=255)
                                                                                                                                               gacgaTACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGATCaa     <  8:189863/149‑1 (MQ=255)
                                                                                                                                                acgaTACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGa         >  4:236119/1‑144 (MQ=255)
                                                                                                                                                acgaTACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATCTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGATCAAg    >  3:243000/1‑149 (MQ=255)
                                                                                                                                                 cgaTACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGATCAAgc   <  7:285713/149‑1 (MQ=255)
                                                                                                                                                  gaTACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGATCAAgcg  >  2:330946/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACCTGGACTCGGGCAAGCACAGCAGCATCGTCACCCGTTTCCCGCCGGAGCCCAATGGTTACCTGCACATCGGCCACGCCAAGTCGATCTGCGTCAACTTCGGCCTGGCCAAGGAATTCGGCGGCGTCTGCCACCTGCGTTTCGACGATACCAACCCGGCCAAGGAAGACCAGGAGTACATCGACGCCATCCAGAGCGATGTCAAATGGCTGGGCTTCGAGTGGGGCGGCGACGTGCGTTACGCCTCCGACTACTTCGACCAGCTGCACGACTGGGCGGTCGAGCTGATCAAGCG  >  NC_002947/3301746‑3302040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑