Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,536,544 C→G 6.7% A61G (GCC→GGC)  PP_3125 → Cro/CI family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,536,5440CG 6.7% 235.6 / 6.6 75A61G (GCC→GGC) PP_3125Cro/CI family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (34/36);  new base G (2/3);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

GGATGACCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAGCCGCCG  >  NC_002947/3536400‑3536692
                                                                                                                                                |                                                                                                                                                     
ggATGACCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCg                                                                                                                                                     >  4:234830/1‑147 (MQ=255)
     aCCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAagag                                                                                                                                                >  1:315134/1‑147 (MQ=255)
      cccTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTGCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGGCGAAGAGctc                                                                                                                                             <  7:205295/149‑3 (MQ=255)
        cTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGc                                                                                                                                           >  7:306055/1‑149 (MQ=255)
        cTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGc                                                                                                                                           <  5:32391/149‑1 (MQ=255)
         tGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGGCGAAGAGc                                                                                                                                               <  8:194763/144‑1 (MQ=255)
         tGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGGCGAAGAGc                                                                                                                                               >  7:194763/1‑144 (MQ=255)
           gAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCgg                                                                                                                                        >  3:116912/1‑149 (MQ=255)
               tGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCag                                                                                                                                    <  4:52790/149‑1 (MQ=255)
                 cTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCagag                                                                                                                                  <  3:176442/149‑1 (MQ=255)
                    gCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCg                                                                                                                               >  4:86201/1‑149 (MQ=255)
                     ccGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGc                                                                                                                              <  2:217514/149‑1 (MQ=255)
                     ccGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGc                                                                                                                              <  8:306055/149‑1 (MQ=255)
                     ccGAAAAGACTGGCATGACCAAAAGCTACCTGTACAAGGTCGAGCGAGGCCTCAACACGCCCTCGATCGCTGCGCCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGCGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGc                                                                                                                              >  1:49546/1‑149 (MQ=255)
                       gAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTa                                                                                                                            <  4:318470/149‑1 (MQ=255)
                        aaaaGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTa                                                                                                                            >  7:4932/1‑148 (MQ=255)
                           aGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGc                                                                                                                        <  7:293091/149‑1 (MQ=255)
                               tGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTgg                                                                                                                    >  8:63726/1‑149 (MQ=255)
                               tGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTgg                                                                                                                    >  6:234125/1‑149 (MQ=255)
                                          aaaGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTAcc                                                                                                         >  6:322783/1‑149 (MQ=255)
                                                aCCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAgcgc                                                                                                   >  2:15632/1‑149 (MQ=255)
                                                 ccTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAgcgc                                                                                                   >  7:173622/1‑148 (MQ=255)
                                                 ccTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAgcgc                                                                                                   <  5:209453/148‑1 (MQ=255)
                                                 ccTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAgcgc                                                                                                   <  3:86201/148‑1 (MQ=255)
                                                    gTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAgg                                                                                               >  2:267839/1‑149 (MQ=255)
                                                     tCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAgg                                                                                               <  2:69327/148‑1 (MQ=255)
                                                       cAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGccc                                                                                            >  5:104910/1‑149 (MQ=255)
                                                       cAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGc                                                                                              <  3:171444/147‑1 (MQ=255)
                                                       cAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGc                                                                                              >  4:171444/1‑147 (MQ=255)
                                                        aaGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCt                                                                                           >  6:50207/1‑149 (MQ=255)
                                                        aaGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCt                                                                                           >  4:73946/1‑149 (MQ=255)
                                                        aaGGTCGAGCCCGGCCTCAACACGCCCTCGATCGCTGCGCCGCTGAAACAGGCGACGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGCCCGGGCAGAGCCGCTACAGCCTCGTGCGCCGTACCGAGCGCCAGGcc                                                                                             >  4:339055/1‑147 (MQ=255)
                                                          ggTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTgg                                                                                         >  7:212261/1‑149 (MQ=255)
                                                           gTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCt                                                                                           >  6:13576/1‑146 (MQ=255)
                                                           gTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCt                                                                                           <  5:13576/146‑1 (MQ=255)
                                                            tCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGtt                                                                                       <  4:116912/149‑1 (MQ=255)
                                                            tCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGtt                                                                                       <  1:15632/149‑1 (MQ=255)
                                                              gAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTgg                                                                                     <  1:245131/149‑1 (MQ=255)
                                                               aGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGc                                                                                    >  8:85726/1‑149 (MQ=255)
                                                                gcgcGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGc                                                                                    >  2:167867/1‑148 (MQ=255)
                                                                  gcgGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGAt                                                                                 <  3:234830/149‑1 (MQ=255)
                                                                      ccTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGc                                                                             <  6:217061/149‑1 (MQ=255)
                                                                        tCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGc                                                                             >  5:78340/1‑147 (MQ=255)
                                                                          aaCACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAg                                                                          <  2:49546/148‑1 (MQ=255)
                                                                            cacGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGc                                                                       >  2:365644/1‑149 (MQ=255)
                                                                                cccTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCggg                                                                   <  5:234125/149‑1 (MQ=255)
                                                                                cccTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCggg                                                                   <  7:85726/149‑1 (MQ=255)
                                                                                cccTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCggg                                                                   <  1:365644/149‑1 (MQ=255)
                                                                                  cTCGATCTCTGCGGCTCTGAAACTGGCGATGGCTCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCATGCCGTGCAGATCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGt                                                                 <  1:307707/149‑1 (MQ=255)
                                                                                  cTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCgggg                                                                  >  6:225738/1‑148 (MQ=255)
                                                                                      aTCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTAcgc                                                             <  6:104910/149‑1 (MQ=255)
                                                                                       tCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTAcgcg                                                            <  8:212261/149‑1 (MQ=255)
                                                                                         gCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCgg                                                           <  1:167867/148‑1 (MQ=255)
                                                                                           tGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑ccc                                                       >  5:322215/1‑149 (MQ=255)
                                                                                             cggcgCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCt                                                      <  3:73946/148‑1 (MQ=255)
                                                                                              ggcgCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCa                                                    <  5:225738/149‑1 (MQ=255)
                                                                                                        cTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGAt                                          <  6:78340/149‑1 (MQ=255)
                                                                                                           gCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTgg                                       >  7:246691/1‑149 (MQ=255)
                                                                                                            cGAGGGCGCTGAACGTCAATGTCGAAGATCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGc                                      <  4:79819/149‑1 (MQ=255)
                                                                                                            cGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGc                                      <  8:246691/149‑1 (MQ=255)
                                                                                                                          gTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCctgct                        >  1:248951/1‑149 (MQ=255)
                                                                                                                           tCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGACTGGTGCGCCGTACCGAGCGCCAGGCCCTGCTTGGCGATGGGCAAGGGCCGGGGTACCCCG‑CCCTCACCAGTCAGATTGGCCAGCGcagccagcag                       >  4:80506/1‑147 (MQ=255)
                                                                                                                                   gAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTc                <  6:322215/148‑1 (MQ=255)
                                                                                                                                   gAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCt               >  3:317154/1‑149 (MQ=255)
                                                                                                                                   gAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCt               >  6:106003/1‑149 (MQ=255)
                                                                                                                                    aaGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTg              <  8:173622/149‑1 (MQ=255)
                                                                                                                                      ctgCTCTTCGGCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGAt            >  3:42086/3‑149 (MQ=255)
                                                                                                                                        gCTGTTCGCCGAAGAGAAGGCCGGTCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTc          <  8:4932/149‑1 (MQ=255)
                                                                                                                                        gCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTc          <  7:63726/149‑1 (MQ=255)
                                                                                                                                         ctctTCGGCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTTGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGAtt           <  4:42086/147‑1 (MQ=255)
                                                                                                                                           ctTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAgc       >  4:290423/1‑149 (MQ=255)
                                                                                                                                           ctTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAgc       >  3:12469/1‑149 (MQ=255)
                                                                                                                                           ctTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACAAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAgc       >  1:331117/1‑149 (MQ=255)
                                                                                                                                            ttCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGGCCCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCa         <  1:267839/147‑1 (MQ=255)
                                                                                                                                              cGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAgc       <  2:248951/146‑1 (MQ=255)
                                                                                                                                                ccGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAGccgccg  >  5:201245/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GGATGACCCTGGAAATGCTGGCCGAAAAGACTGGCATGACCAAAAGCTACCTGTCCAAGGTCGAGCGCGGCCTCAACACGCCCTCGATCGCTGCGGCGCTGAAACTGGCGAGGGCGCTGAACGTCAATGTCGAAGAGCTCTTCGCCGAAGAGCAGGCCGGGCAGAGCCGCTACAGCCTGGTGCGCCGTACCGAGCGCCAGGCCCTGGTTGGCGATGGGCAAGGGCCGGGGTACGCGG‑CCCTCACCAGTCAGATTGGCCAGCGCAGCCTGCTGCCGTTTCTGATTCAGCCGCCG  >  NC_002947/3536400‑3536692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑