Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,541,778 C→T 8.0% L315L (TTG→TTA)  galE ← UDP‑glucose 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,541,7780CT 8.0% 146.0 / 2.6 50L315L (TTG→TTA) galEUDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base T (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGAACCTGATCGGGACAGTGTTGAACAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAGTGATCTGT  >  NC_002947/3541633‑3541925
                                                                                                                                                 |                                                                                                                                                   
cGGTGAACCTGATCGGGACAGTGTTGAACAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAAc                                                                                                                                                  <  3:214865/149‑1 (MQ=255)
                gACAGTGTTGAACAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCAt                                                                                                                                  <  3:53896/149‑1 (MQ=255)
                      gtTGAACAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGc                                                                                                                                                     <  8:343606/124‑1 (MQ=255)
                      gtTGAACAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGc                                                                                                                                                     >  7:343606/1‑124 (MQ=255)
                         gAACAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACaa                                                                                                                         >  1:319602/1‑149 (MQ=255)
                          aaCAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAAt                                                                                                                        <  7:85739/149‑1 (MQ=255)
                            cAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCt                                                                                                                      <  4:206082/149‑1 (MQ=255)
                                      gaACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGt                                                                                                              >  4:141934/1‑147 (MQ=255)
                                         cAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGc                                                                                                         <  3:339825/149‑1 (MQ=255)
                                                     tATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGgcg                                                                                             >  6:190001/1‑149 (MQ=255)
                                                              gCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGccagcccag                                                                                    >  4:54894/1‑149 (MQ=255)
                                                               ccTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGccagcccag                                                                                    >  1:73306/1‑148 (MQ=255)
                                                                cTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCa                                                                                  <  3:265192/149‑1 (MQ=255)
                                                                cTTCTGGCAATACGCTTTCACGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCTCTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCa                                                                                  <  2:352726/149‑1 (MQ=255)
                                                                 ttCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCAc                                                                                 >  8:182079/1‑149 (MQ=255)
                                                                  tCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCAcc                                                                                >  1:318779/1‑149 (MQ=255)
                                                                      gCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCaa                                                                                                                                                   <  7:60402/78‑1 (MQ=255)
                                                                      gCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCaa                                                                                                                                                   >  8:60402/1‑78 (MQ=255)
                                                                        aaTACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATg                                                                          >  3:353616/1‑149 (MQ=255)
                                                                              cTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGTAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTg                                                                    >  4:170407/1‑149 (MQ=255)
                                                                              cTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGTAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTg                                                                    >  4:164704/1‑149 (MQ=255)
                                                                                  cAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTc                                                                 <  2:174180/148‑1 (MQ=255)
                                                                                    ggCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGgc                                                                                              <  2:179637/117‑1 (MQ=255)
                                                                                    ggCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGgc                                                                                              >  1:179637/1‑117 (MQ=255)
                                                                                     gcgcAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTc                                                                 >  7:354257/1‑145 (MQ=255)
                                                                                     gcgcAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTc                                                                 <  8:354257/145‑1 (MQ=255)
                                                                                           ccTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCt                                                                  <  6:88369/138‑1 (MQ=255)
                                                                                           ccTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCt                                                                  >  5:88369/1‑138 (MQ=255)
                                                                                               gtgCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGccacca                                                    <  5:190001/148‑1 (MQ=255)
                                                                                               gtgCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAg                                                   >  3:12913/1‑149 (MQ=255)
                                                                                                        cTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCgg                                          >  5:326881/1‑149 (MQ=255)
                                                                                                          gAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGt                                        >  7:296372/1‑149 (MQ=255)
                                                                                                                tATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGa                                                                                                            >  3:103716/1‑75 (MQ=255)
                                                                                                                tATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGa                                                                                                            <  4:103716/75‑1 (MQ=255)
                                                                                                                       ccAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCcggcgcggcg                           >  7:257469/1‑149 (MQ=255)
                                                                                                                           cTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCtgt                       <  4:267613/149‑1 (MQ=255)
                                                                                                                           cTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCtgt                       <  2:318779/149‑1 (MQ=255)
                                                                                                                                  ccTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCaa                <  4:12913/149‑1 (MQ=255)
                                                                                                                                   cTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGTCGCTGTGCGTCAAg               <  7:182079/149‑1 (MQ=255)
                                                                                                                                      cAGGGATACTGTAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAggg             <  3:164704/148‑1 (MQ=255)
                                                                                                                                      cAGGGATACTGTAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAggg             <  3:170407/148‑1 (MQ=255)
                                                                                                                                      cAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAggg             >  2:166136/1‑148 (MQ=255)
                                                                                                                                      cAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAggg             <  2:319602/148‑1 (MQ=255)
                                                                                                                                      cAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAgg              <  5:53110/147‑1 (MQ=255)
                                                                                                                                        gggATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAg          <  2:73306/149‑1 (MQ=255)
                                                                                                                                           aTACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAGTGa       >  4:145383/1‑149 (MQ=255)
                                                                                                                                            tACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAGTGAt      <  4:353616/149‑1 (MQ=255)
                                                                                                                                            tACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAGTGAt      <  3:54894/149‑1 (MQ=255)
                                                                                                                                               tGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAgg                                                                                                <  7:107336/56‑1 (MQ=255)
                                                                                                                                               tGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAgg                                                                                                >  8:107336/1‑56 (MQ=255)
                                                                                                                                               tGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAGTGATCTg   >  6:364401/1‑149 (MQ=255)
                                                                                                                                               tGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAGTGATCTg   >  1:277398/1‑149 (MQ=255)
                                                                                                                                                 cAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAGTGATCTGt  <  2:277398/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGGTGAACCTGATCGGGACAGTGTTGAACAAGGCCGACGAACAGCCACGCACCTATTACTGAGCCTTCTGGCAATACGCTTTCAGGCGCAGCCTTGTGCTGCGCCTGAAAGCTATGAGGCCAACTCAGCGCCTCCAGGGATACTGCAACTCCCACTGCCACGCATGCCGCACAATCTGCTCCAGTGAGGCGAATGCAGGGCGCCAGCCCAGCACCTGCATGGCCTTGCTCGCATCCGCCACCAGCCGTGGCGGGTCGCCGGCGCGGCGCTGTGCGTCAAGGGCAGTGATCTGT  >  NC_002947/3541633‑3541925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑