Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,542,321 C→T 6.6% P134P (CCG→CCA)  galE ← UDP‑glucose 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,542,3210CT 6.6% 269.1 / 6.3 91P134P (CCG→CCA) galEUDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (44/41);  new base T (2/4);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

GATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCT  >  NC_002947/3542173‑3542469
                                                                                                                                                    |                                                                                                                                                      
gATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAc                                                                                                                                                        >  3:95401/1‑149 (MQ=255)
 aTGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACAGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACg                                                                                                                                                       >  1:218252/1‑149 (MQ=255)
       ttCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGtt                                                                                                                                                   >  4:19246/1‑147 (MQ=255)
         ccGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGag                                                                                                                                                <  3:19246/148‑2 (MQ=255)
         ccGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACgg                                                                                                                                                      <  5:372560/142‑1 (MQ=255)
         ccGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACgg                                                                                                                                                      >  6:372560/1‑142 (MQ=255)
          cGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagca                                                                                                                                              <  6:300142/149‑4 (MQ=255)
          cGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagc                                                                                                                                               <  3:193856/148‑3 (MQ=255)
          cGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGt                                                                                                                                                    <  3:6667/143‑1 (MQ=255)
          cGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGt                                                                                                                                                    >  4:6667/1‑143 (MQ=255)
            gCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagcaaa                                                                                                                                            >  1:122766/1‑144 (MQ=255)
             cTCGTGACGCTCGCCTAGCTGTCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGcc                                                                                                                                           <  4:214354/149‑1 (MQ=255)
             cTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagcaaat                                                                                                                                           <  2:122766/149‑7 (MQ=255)
                   aCGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTg                                                                                                                                     <  5:319823/148‑1 (MQ=255)
                    cGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGgt                                                                                                                                   <  4:95401/149‑1 (MQ=255)
                    cGCTCGCCTAGCTGGCCTTCCGGGGCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGgt                                                                                                                                   <  8:347563/149‑1 (MQ=255)
                      cTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGgtgt                                                                                                                                 <  7:8292/149‑1 (MQ=255)
                          ccTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGATTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGCTGT‑GTGc                                                                                                                            >  4:223944/1‑149 (MQ=255)
                            tAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCtt                                                                                                                          >  8:111645/1‑149 (MQ=255)
                             aGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAACCCATAGGAACGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTCCtt                                                                                                                          >  3:319630/1‑148 (MQ=255)
                             aGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCtt                                                                                                                          >  6:107591/1‑148 (MQ=255)
                                  gCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCatcga                                                                                                                    >  2:247044/1‑149 (MQ=255)
                                    cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAc                                                                                                                                                        <  4:149010/113‑1 (MQ=255)
                                    cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAc                                                                                                                                                        >  3:149010/1‑113 (MQ=255)
                                    cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCatcgatc                                                                                                                  >  3:278952/1‑149 (MQ=255)
                                     ttCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCatcgatcg                                                                                                                 >  4:111589/1‑149 (MQ=255)
                                               gcgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTg                                                                                                       >  2:116472/1‑149 (MQ=255)
                                               gcgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTg                                                                                                       >  2:46291/1‑149 (MQ=255)
                                                  cccGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCgg                                                                                                    >  8:170677/1‑149 (MQ=255)
                                                   ccGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGAACGGCCCC‑TTGGTGT‑GTGCTTCATAGATAGGAACGTACTACggg                                                                                                   >  7:232267/1‑149 (MQ=255)
                                                   ccGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCACGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCggg                                                                                                   <  1:116472/149‑1 (MQ=255)
                                                         cATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTg                                                                                                >  3:18534/1‑146 (MQ=255)
                                                         cATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTg                                                                                                <  4:18534/146‑1 (MQ=255)
                                                         cATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGcc                                                                                              <  1:46291/148‑1 (MQ=255)
                                                          aTTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCatc                                                                                                                      >  7:70665/1‑123 (MQ=255)
                                                          aTTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCatc                                                                                                                      <  8:70665/123‑1 (MQ=255)
                                                          aTTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCg                                                                                             >  2:212736/1‑148 (MQ=255)
                                                                   gcgcAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcgg                                                                                   <  1:212736/149‑1 (MQ=255)
                                                                   gcgcAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcgg                                                                                   <  2:362941/149‑1 (MQ=255)
                                                                      cAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCAc                                                                                <  3:111589/149‑1 (MQ=255)
                                                                         gCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTc                                                                              >  7:261716/1‑148 (MQ=255)
                                                                          cTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑ATGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCga                                                                            <  7:111645/149‑1 (MQ=255)
                                                                           ttACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCg                                                                             >  7:245437/1‑147 (MQ=255)
                                                                           ttACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCg                                                                             >  3:140120/1‑147 (MQ=255)
                                                                           ttACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCg                                                                             >  6:43942/1‑147 (MQ=255)
                                                                                gACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa                                                                      <  4:140120/149‑1 (MQ=255)
                                                                                gACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa                                                                      <  3:223944/149‑1 (MQ=255)
                                                                                 aatttaaaCCATACGCCCGGTCGAATTCCTCAAGAATCTGCTCGTCCATCCATTTGCTCAACCCATACTGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAac                                                                     <  8:232267/144‑1 (MQ=255)
                                                                                 aCTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTGCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAac                                                                     <  1:240221/149‑1 (MQ=255)
                                                                                 aCTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTACATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa                                                                      >  1:303673/1‑148 (MQ=255)
                                                                                   ttCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAacac                                                                   >  7:207478/1‑149 (MQ=255)
                                                                                   ttCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAacac                                                                   >  6:235822/1‑149 (MQ=255)
                                                                                   ttCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGATGATCGGCCCC‑TAGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAaca                                                                    >  6:360884/1‑148 (MQ=255)
                                                                                     cAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcgg                                                                                   >  8:126326/1‑131 (MQ=255)
                                                                                     cAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcgg                                                                                   <  7:126326/131‑1 (MQ=255)
                                                                                     cAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAacac                                                                   >  6:92254/1‑147 (MQ=255)
                                                                                     cAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAacac                                                                   >  6:174559/1‑147 (MQ=255)
                                                                                     cAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGGTGCCGTATACGGCGGCACTCGAAGAAAacac                                                                   <  1:247044/147‑1 (MQ=255)
                                                                                      aaaCCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCa                                                                 <  8:245437/148‑1 (MQ=255)
                                                                                       aaCCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCAATACGGGTTGATAGGCCCC‑TTGGTGT‑GTGCTTCATAGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAgg                                                               >  1:352390/1‑149 (MQ=255)
                                                                                          cATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc                                                            >  5:89219/1‑149 (MQ=255)
                                                                                           aTAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGcc                                                           <  5:167247/149‑1 (MQ=255)
                                                                                               gCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACGCGAAGAAAACACCAGGTGCCTGAt                                                       >  4:365298/1‑149 (MQ=255)
                                                                                                 ccGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGc                                                     >  6:334172/1‑149 (MQ=255)
                                                                                                   ggTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGAACTCGAAGAAAACACACGGTGCCTGATGcct                                                   >  8:327611/1‑148 (MQ=255)
                                                                                                       gAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCAt                                               >  4:56562/1‑149 (MQ=255)
                                                                                                        aaGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCGCC‑TTGGTGT‑GTGCTTAATAGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCAtt                                              <  4:319630/149‑1 (MQ=255)
                                                                                                        aaGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCAtt                                              <  2:218252/149‑1 (MQ=255)
                                                                                                          gTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGa                                            <  5:235822/149‑1 (MQ=255)
                                                                                                            ccTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGAc                                           <  7:170677/148‑1 (MQ=255)
                                                                                                             cTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCg                                                                                                     <  7:356061/89‑1 (MQ=255)
                                                                                                             cTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCg                                                                                                     >  8:356061/1‑89 (MQ=255)
                                                                                                             cTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCa                                         >  4:351648/1‑149 (MQ=255)
                                                                                                             cTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCa                                         >  5:117927/1‑149 (MQ=255)
                                                                                                                aaGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATg                                       <  6:89219/148‑1 (MQ=255)
                                                                                                                       tGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAg                                                                >  6:261490/1‑116 (MQ=255)
                                                                                                                         cTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTgcagc                              <  8:261716/148‑1 (MQ=255)
                                                                                                                           cGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                           <  3:351648/149‑1 (MQ=255)
                                                                                                                            gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCa                                                                 >  1:125652/1‑110 (MQ=255)
                                                                                                                            gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCa                                                                 <  2:125652/110‑1 (MQ=255)
                                                                                                                            gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAggg                          <  5:92254/149‑1 (MQ=255)
                                                                                                                            gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                           <  5:174559/148‑1 (MQ=255)
                                                                                                                             aCCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAggg                          >  4:110820/1‑148 (MQ=255)
                                                                                                                               catccatTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGTGGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGa                         >  1:76618/1‑148 (MQ=255)
                                                                                                                                   catTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTTCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGt                   <  7:327611/149‑1 (MQ=255)
                                                                                                                                   catTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGt                   <  3:222466/149‑1 (MQ=255)
                                                                                                                                    atTTGCTCAACCCATACGTGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg                  <  6:117927/149‑1 (MQ=255)
                                                                                                                                    atTTGCTCAACCCATACGGGTTGATCGGCCCCTTTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTACAGCAGGGAAAgg                     >  1:44543/1‑147 (MQ=255)
                                                                                                                                    atTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg                  <  3:56562/149‑1 (MQ=255)
                                                                                                                                    atTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTACAGCAGGGAAAgg                     <  2:44543/146‑1 (MQ=255)
                                                                                                                                            aaCCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGa           <  5:26209/148‑1 (MQ=255)
                                                                                                                                               ccATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACgtt       <  8:207478/149‑1 (MQ=255)
                                                                                                                                                cATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGAAGCAGGGAAAGGGTCGCTGCGACgttg      >  4:119640/1‑149 (MQ=255)
                                                                                                                                                   aCGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGCCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTc   >  2:137009/1‑149 (MQ=255)
                                                                                                                                                    cGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCt  <  5:334172/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCC‑TTGGTGT‑GTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTTGTTCT  >  NC_002947/3542173‑3542469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑